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Influence of the Lipid Environment on Insulin Binding to Placental Membranes from Normal and Diabetic Mothers

  • Gernot Desoye
  • Peter A. M. Weiss
Chapter
Part of the Trophoblast Research book series (TR)

Abstract

It is generally accepted that the function of membrane proteins is affected by the fluidity of the membrane (Sandermann, 1978; Kates and Kuksis, 1980; Shinitzky et al., 1980; Chapman, 1983). Temperature (Lee, 1977), the protein to lipid content (Shinitzky et al., 1980) and the lipid composition (Phillips et al., 1969; Borochov et al., 1979) are the major determinants of membrane fluidity. Effects of fluidity on receptor specificity and affinity have been reported for the thyrotropin receptor (Mehdi et al., 1977; Lee et al., 1978), for the serotonin receptor (Heron et al., 1980) and for the insulin receptor (Amatruda and Finch, 1979; Grunfeld et al., 1981; McCaleb and Donner, 1981; Ginsberg et al., 1981; Gould et al., 1982; Bar et al., 1984). The studies directed to the modulation of membrane proteins by the lipid environment have been accomplished on cultured cells by dietary manipulations leading to a modification of either the fatty acid composition or phospholipid headgroups. Corresponding studies with isolated membranes used physical techniques which altered the bulk fluidity of the membrane or by treatment of the membranes with phospholipases (Gould and Ginsberg, 1984). In the present study we chose a different approach. The affinity of insulin receptors from various tissues has been repeatedly shown to be altered in diabetes mellitus (Andreani et al., 1981). We analyzed the insulin receptors in placental membranes from normal and diabetic mothers and correlated the receptor affinities with parameters which are known to determine membrane fluidity. Thus, we did not study the insulin receptor system in an artificially altered lipid environment but investigated how the receptor affinity is affected by an in vivo modification of the membrane as a result of a pathological state.

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References

  1. Amatruda, J.M. and Finch, E.D. (1979) Modulation of hexose uptake and insulin action by cell membrane fluidity. J. Biol. Chem. 254, 2619–2625.PubMedGoogle Scholar
  2. Andreani, D., DePirro, R., Lauro, R., Olefsky, J.M., and Roth, J. (eds.) (1980) Current Views on Insulin Receptors, New York, Academic Press.Google Scholar
  3. Arkejsteijn, C.L.M. (9176) A kinetic method of serum 5’-nucleotidase using stabilised gluatmate dehydrogenase. J. Clin. Chem. Clin. Biochem. 14, 155–158.Google Scholar
  4. Bar, R.S., Dolash, S., Spector, A.A., Kaduce, T.L., and Figard, Ph.D. (1984) Effects of membrane lipid unsaturation on the interactions of insulin and multiplicating stimulating activity with endothelial cells. Biochim. Biophys. Acta 804, 466473.Google Scholar
  5. Borochov, H., Abbott, R.E., Schachter, D., and Shinitzky, K. (1979) Modulation of erythrocyte membrane proteins by membrane cholesterol and lipid fluidity. Biochem. 18, 251–255.CrossRefGoogle Scholar
  6. Bowers, G.N., Jr. (1959) Measurement of isocitric dehydrogenase activity in body fluids. Clin. Chem. 5, 509–518.PubMedGoogle Scholar
  7. Bowers, G.N., Jr., and McComb, R.B. (1966) A continuous spectrophotometric method for measuring the activity of serum alkaline phosphatase. Clin. Chem. 12, 7089.Google Scholar
  8. Chapman, D. (1983) Biomembrane fluidity: The concept and its development. In: Membrane Fluidity in Biology, Vol. 2, New York, Academic Press, pp. 5–42.Google Scholar
  9. Duran-Garcia, S., Nieto, J.G., and Cabello, A.M. (1979) Effect of gestational diabetes on insulin receptors in human placenta. Diabetologia 16, 87–91.PubMedCrossRefGoogle Scholar
  10. Fishman, W.H., Kato, K., Anstiss, C.L., and Green, S. (1967) Human serum betaglucuronidase; its measurement and some of its properties. Clin. Chim. Acta 15, 435–447.PubMedCrossRefGoogle Scholar
  11. Fiske, C.H. and Subbarow, Y. (1926) The colorimetric determination of phosphorus. J. Biol. Chem. 66, 375–400.Google Scholar
  12. Ginsberg, B., Brown, T.J., Simon, I., and Spector, A.A. (1981) Effect of the membrane lipid environment on the properties of insulin receptors. Diabetes 30, 773–780.PubMedCrossRefGoogle Scholar
  13. Ginsberg, B.H., Jabour, J., and Spector, A.A. (1982) Effect of alterations in membrane lipid unsaturation on the properties of the insulin receptor of Ehrlich ascites cells. Biochim. Biophys. Acta 690, 157–164.PubMedCrossRefGoogle Scholar
  14. Gould, R.J., Ginsberg, B.H., and Spector, A.A. (1982) Lipid effects on the binding properties of a reconstituted insulin receptor. J. Biol. Chem. 257, 477–484.PubMedGoogle Scholar
  15. Gould, R.J. and Ginsberg, B.H. (1984) Biochemistry and analysis of membrane phospholipids: Application to membrane receptors. In: Membranes, Detergents, and Receptor Solubilization, (eds.), J.C. Venter and L.C. Harrison, New York, Alan R. Liss, pp. 65–83.Google Scholar
  16. Grunfeld, C., Baird, K.L., and Kahn, C.R. (1981) Maintenance of 3T3–L1 cells in culture media containing saturated fatty acids decreases insulin binding and insulin action. Biochem. Biophys. Res. Commun. 103, 219–226.PubMedCrossRefGoogle Scholar
  17. Haour, F., and Bertrand, J. (1974) Insulin receptors in the plasma membranes of human placenta. J. Clin. Endocrinol. Metab. 38, 334–337.PubMedCrossRefGoogle Scholar
  18. Harrison, L.C., Billington, T., Clark, S., Nichols, R., East, I., and Martin, F.I.R. (1977) Decreased binding of insulin by receptors on placental membranes from diabetic mothers. J. Clin. Endocrinol. Metab. 44, 206–209.Google Scholar
  19. Harrison, L.C. and Itin, A. (1980) Purification of the insulin receptor from human placenta by chromatography on immobilized wheat germ lectin and receptor antibodies. Biol. Chem. 255, 12066–12072.Google Scholar
  20. Heron, D.S., Shinitzky, M., Hershkowitz, M., and Samuel, D. (1980) Lipid fluidity markedly modulates the binding of serotonin to mouse brain membranes. Proc. Natl. Acad. Sci. USA 77, 7463–7467.PubMedCrossRefGoogle Scholar
  21. Kates, M. and Kuksis, A., (eds.) (1980) Membrane Fluidity: Biophysical Techniques and Cellular Regulation, Clifton, New Jersey, The Humana Press.Google Scholar
  22. Lands, W.E.M. (1980) Fluidity of membrane lipids. In: Membrane Fluidity. Biophysical Techniques and Cellular Regulation, (eds.), M. Kates and A. Kuksis, Clifton, New Jersey, The Humana Press, pp. 69–73.Google Scholar
  23. Lee, A.G. (1977) Lipid phase transitions and phase diagrams. I. Lipid phase transitions. Biochim. Biophys. Acta 472, 237–281.PubMedCrossRefGoogle Scholar
  24. Lee, G., Consiglio, E., Habig, W., Dyer, S., Hardegree, C., and Kohn, L.D. (1978) Structure-function studies of receptors for thyrotropin and tetanus toxin. Lipid modulation of effect or binding to glycoprotein receptor component. Biochem. Biophys. Res. Commun. 83, 313–320.PubMedCrossRefGoogle Scholar
  25. Lowry, O.H., Rosenbrough, N.J., Farr, A.L., and Randall, R.J. (1951) Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265–275.PubMedGoogle Scholar
  26. McCaleb, M.L. and Donner, D.B. (1981) Affinity of the hepatic insulin receptor is influenced by membrane phospholipids. J. Biol. Chem. 256, 11051–11057.PubMedGoogle Scholar
  27. Mehdi, S.Q., Nussey, S.S., Shindelman, J.E., and Kriss, J.P. (1977) Influence of lipid substitution on thyrotropin-receptor interactions in artificial vesicles. Endocrinol. 101, 1406–1412.CrossRefGoogle Scholar
  28. Morrison, W.R. and Smith, L.M. (1964) Preparation of fatty acid methyl esters and dimethylacetals from lipids with boronfluoride-methanol. J. Lipid Res. 5, 600608.Google Scholar
  29. Nelson, D.M., Smith, R.M., and Jarett, L. (1978) Nonuniform distribution and grouping of insulin receptors on the surface of human placental syncytial trophoblast. Diabetes 27, 530–538.PubMedCrossRefGoogle Scholar
  30. Phillips, M.C., Williams, R.M., and Chapman, D. (1969) On nature of hydrocarbon chain motions in lipid liquid crystals. Chem. Phys. Lipids 3, 234–244.CrossRefGoogle Scholar
  31. Posner, B.I. (1973) Insulin receptors in human and animal placental tissue. Diabetes 23, 209–217.Google Scholar
  32. Sandermann, H., Jr. (1978) Regulation of membrane enzymes by lipids. Biochim. Biophys. Acta 515, 209–237.PubMedCrossRefGoogle Scholar
  33. Shinitzky, M. and Inbar, M. (1976) Microviscosity parameters and protein mobility in biological membranes. Biochim. Biophys. Acta 43, 133–149.CrossRefGoogle Scholar
  34. Shinitzky, M. and Henkart, P. (1980) Fluidity of cell membranes–current concepts and trends. Int. Rev. Cytol. 60, 121–147.CrossRefGoogle Scholar
  35. Shinitzky, M., Borochov, H., and Wilbrandt, W. (1980) Lipid fluidity as a physiological regulator of membrane transport and enzyme activities. In: Membrane Transport in Erythrocytes, (eds.), H.H. Ussing and J.O. Wieth, Copenhagen, Munksgaard, pp. 91–107.Google Scholar
  36. Siedel, J., Schlumberger, H., Klose, S., Ziegenhorn, J., and Wahlefeld, A.W. (1981) Improved reagent for the enzymatic determination of serum cholesterol. J. Clin. Chem. Clin. Biochem. 19, 838–839.Google Scholar
  37. Stubbs, C.D. and Smith, A.D. (1984) The modification of mammalian membrane polyunsaturated fatty acid composition in relation to membrane fluidity and function. Biochim. Biophys. Acta 779, 89–137.PubMedCrossRefGoogle Scholar
  38. Veerkamp, J.H. and Broekhyse, R.M. (1976) Technique for the analysis of membrane lipids. In: Biochemical Analysis of Membranes, (ed.), A.H. Maddy, London, Chapman and Hall, pp. 252–282.Google Scholar
  39. Warren, L. (1963) Thiobarbituric acid assay of sialic acids. Meth. Enzymol. 6, 463–466.CrossRefGoogle Scholar
  40. Whitsett, J.A. and Lessard, J.L. (1978) Characteristics of the microvillus brush border of human placenta: Insulin receptor localization in brush border membranes. Endocrinol. 103, 1458–1468.CrossRefGoogle Scholar
  41. Williams, P.F., and Turtle, J.R. (1979) Purification of the insulin receptor from human placental membranes. Biochim. Biophys. Acta 579, 367–374.PubMedCrossRefGoogle Scholar
  42. Wren, J.J. and Szczepanowska, A.D. (1964) Chromatography of lipids in presence of an antioxidant, 4-methyl-2,6-di-tert-butylphenol. J. Chromatog. 14, 405–410.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • Gernot Desoye
    • 1
  • Peter A. M. Weiss
    • 1
  1. 1.Department of Obstetrics and GynecologyUniversity of GrazGrazAustria

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