The Placenta as a Model for Toxicity Screening of New Molecules

  • Rebecca Beaconsfield
  • Bojana Cemerikic
  • Olga Genbacev
  • Vojin Sulovic
Part of the Trophoblast Research book series (TR)


Our in vitro model of placental tissue explants is designed to assess the direct acute effect of drugs on trophoblast cell metabolism (Genbacev et al., 1982). The possible clinical implications of the information obtained are indirect, but it could be inferred that alterations in trophoblast cell metabolism affects in turn fetal well being. Our purpose was to evaluate, using the described experimental model, the direct effect of two groups of drugs that are believed to have adverse effects on fetal growth: negative /morphine and morphine-like substances and nicotine/ and positive /beta-mimetic drugs. The parameters selected were glucose uptake, lactate production, 14C-Leucine uptake and incorporation into total soluble proteins, placental hormone release and intracellular cAMP content (Genbacev et al., 1982).


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  1. Ahmend, M.S., Byrne, W.L., and Klee, W.A. (1981) Solubilization of opiate receptors from human palcenta. Placenta. (Suppl. 3), 113 - 122.Google Scholar
  2. Barnwell, S.L. and Sastry, B.V.R. (1983) Depression of amino acid uptake in human placental villus by cocaine, morphine and nicotine. Trophoblast Res. 1, 101 - 120.Google Scholar
  3. Beaconsfield, P. (1979) Drugs and the placenta - A personal view. In: Placenta - A Neglected Experimental Animal, (eds.) P. Beaconsfield and C. Villee, Pergamon Press, pp. 105 - 108.Google Scholar
  4. Beaconsfield, P. (1982) Drugs and placental metabolism - An introduction to the reports of preliminary work. In: Placenta - The Largest Human Biopsy, (eds.), R. Beaconsfield and G. Birdwood, Pergamon Press, pp. 79 - 90.Google Scholar
  5. Belleville, F., Lasbennes, A., Nabet, P., and Paysant, P. (1978) HCS-HCG regulation in cultured placenta. Acta Endocrinol. (Kbh) 88, 169 - 176.Google Scholar
  6. Berg, G., Andersson, R.G.G., and Ryden, G. (1985) Beta-Adrenergic receptors in human myometrium during pregnancy: Changes in the number of receptors after beta-mimetic treatment. Am. J. Obstet. Gynecol. 151, 392 - 396.PubMedCrossRefGoogle Scholar
  7. Burd, I.L., Jones, M.D., Jr., Simmons, M.A., Makowski, E.L., Meschia, G., and Bataglia, F.C. (1975) Placental production and foetal utilization of lactate and pyruvate. Nature 254, 710 - 711.PubMedCrossRefGoogle Scholar
  8. Cemerikic, B., Genbacev, O., and Sulovic, V. (1984) In vitro effect of ritodrinum on cAMP concentration in human placentas of different gestational age. Exp. Clin. Endocrinol. 87, 72 - 77.Google Scholar
  9. Falkay, G. and Kovacs, L. (1983) Beta-adrenergic receptors in early human placenta characterization of (3H)-dihydroalprenolol binding. Life Sci. 32, 1583 - 4590.PubMedCrossRefGoogle Scholar
  10. Genbacev, O., Cemerikic, B., Movsesijan, M., and Sulovic, V. (1981) Protein synthesis by human fetal lung. Am. J. Obstet. Gynecol. 134, 41 - 46.Google Scholar
  11. Genbacev, O., Cemerikic, B., Poljakovic, Lj., and Ilic, D. (1982) Incubation studies using placental tissue slices. In: Placenta - The Largest Human Biopsy, (eds.), R. Beaconsfield, and G. Birdwood, Pergamon Press, pp. 113 - 126.Google Scholar
  12. Ginsburg, J. and Jeacock, M.K. (1968) Effect of epinephrine on placental carbohydrate metabolism. Am. J. Obstet. Gynecol. 100, 357 - 368.PubMedGoogle Scholar
  13. Hasselmeyer, E.G., Meyer, M.B., Catz, C., and Longo, L.D. (1979) Pregnancy and infant health. In: Smoking and Health: A Report of the Surgeon General, (ed.), J.M. Pinney, United States Department of Health, Education and Welfare Publication No. ( PHS ) 79 - 5006.Google Scholar
  14. Lahav, M., Sofer, N., Brandes, J.M., Paldi, E., and Barzilai, D. (1980) Placenta concentrations of progesterone, estradiol-17 beta, and cyclic AMP at delivery. Obstet. Gynecol. 56, 616 - 620.PubMedGoogle Scholar
  15. Lorincz, A.B. and Kuttner, R.E. (1968) Comparative studies on free amino acids in female reproductive tissues. Am. J. Obstet. Gynecol. 101, 462 - 472.PubMedGoogle Scholar
  16. Olubadewo, J.O. and Sastry, B.V.R. (19 8) Human placental cholinergic system; Stimulation-secretion coupling for release of acetylcholine form isolated placental villus. J. Pharm. Exp. Therap. 204, 433 - 445.Google Scholar
  17. Padbury, J.F., Hobel, C.J., Diakomanolis, E.S., Lam, R.W., and Fisher, D.A. (1981) Ontogenesis of beta-adrenergic receptors in the ovine placenta. Am. J. Obstet. Gynecol. 139, 459-464.Google Scholar
  18. Satch, K. and Ryan, K.J. (1971) Adenyl cyclase in the human placenta. Biochim. Biophys. Acta 224, 618 - 626.Google Scholar
  19. Schocken, D.D., Caron, M.G., and Lefkowitz, R.J. (1980) The human placenta. A rich source of beta-adrenergic receptors: Characterization of the receptors in particulate and solubilized preparations. J. Clin. Endocrinol. Metab. 50, 1082-1088.Google Scholar
  20. Shu-Rong, Z., Bremme, K., Enorth, P., and Nordberg, A. (1982) The regulation in vitro of placental release of human chorionic gonadotropin, placental lactogen and prolactin: Effects of an andrenergic beta-receptors agonist and antagonist. Am. J. Obstet. Gynecol. 143, 444 - 450.PubMedGoogle Scholar
  21. Stone, M., Salerno, L., Green, M., and Zelson, C. (1971) Narcotic addition in pregnancy. Am. J. Obstet. Gynecol. 109, 716 - 723.PubMedGoogle Scholar
  22. Tuchmann-Duplessis, H. (1975) Drugs acting on the central nervous system. In: Drug Effects on the Fetus, Publishing Sciences Group, Acton, MA, pp. 142 - 194.Google Scholar
  23. Valette, A., Reme, J.M, Pontonnier, G., and Cros, J. (1980) Specific binding for opiate-like drugs in the placenta. Biochem. Pharm. 29, 2657 - 2661.PubMedCrossRefGoogle Scholar
  24. Wansbrough, H., Nakanishi, H., and Wood, C. (1968) The effect of adrenergic receptors blocking drugs on the human uterus. J. Obstet. Gynaecol. Br. Cmwlth. 75, 189-197.Google Scholar
  25. Whitsett, J.A., Johnson, C.L., Noguchi, A., Darovec-Beckerman, C., and Costello, M. (1980) Beta-adrenergic receptors and catecholamines sensitive adenyate cyclase of the human placenta. J. Clin. Endocrinol. Metab. 50, 27-32.Google Scholar

Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • Rebecca Beaconsfield
    • 1
  • Bojana Cemerikic
    • 1
  • Olga Genbacev
    • 1
  • Vojin Sulovic
    • 1
  1. 1.INEP Department of EndocrinologyImmunology and NutritionZemunYugoslavia

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