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Characterization of Maternal Trophoblast Lymphocyte Cross-Reactive (TLX) Immunity

  • Donald S. Torry
  • John A. McIntyre
  • W. Page Faulk
  • Peter R. McConnachie
Chapter
Part of the Trophoblast Research book series (TR)

Abstract

Women suffering from idiopathic recurrent spontaneous abortions may be grouped as primary (1°) or secondary (2°) aborters (McIntyre and Faulk, 1984; McIntyre et al., 1984). Women classified as 2° aborters have had children, stillbirths or premature births before their abortions began. Unlike 1° aborters, 2° aborters do not share HLA antigens with their spouses and they have high-titred complement dependent cytotoxicity (CDC) and antibody dependent cell-mediated cytotoxicity (ADCC) reactivity against paternal and non-paternal lymphoyctes even when not pregnant (McConnachie and McIntyre, 1984; McIntyre et al., 1984; McIntyre et al., 1986). Although multiparous women are the primary source of HLA typing sera, only 20–60% actually produce demonstrable antibodies and most of these disappear soon after delivery (Amon and Kostyu, 1980). Study of 2° aborters’ antibodies on HLA select cell panels before and after absorption with trophoblast membranes has shown patterns of reactivity which indicate specificities for trophoblast lymphocyte crossreactive (TLX) antigens (McIntyre and Faulk, 1982; McIntyre et al., 1983; McIntyre et al., 1985; Faulk and McIntyre, 1983).

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References

  1. Amos, D.B. (1979) In: NIAID Manuel of Tissue Typing Techniques, (ed.), J.G. Ray, NIH publication No. 80–545, 42.Google Scholar
  2. Davies, M. (1985) Antigenic analysis of immune complex formed in normal human pregnancy. Clin. Exp. Immunol. 61, 406–415.PubMedCentralPubMedGoogle Scholar
  3. Davies, M. and Browne, C.M. (1985) Anti-trophoblast antibody responses during normal human pregnancy. J. Reprod. Immunol. 7, 285–297.PubMedCrossRefGoogle Scholar
  4. Davies, M. and Browne, C.M. (1985a) Pregnancy associated non-specific immunosuppression. Kinetics of the generation and identification of the active factors. Am.J. Reprod. Immunol. Microbiol. 9, 77–83.PubMedGoogle Scholar
  5. Davies, M. and Browne, C.M. (1985b) Pregnancy associated non-specific immunosuppression. Mechanism for the activation of the immunosuppressive factiors. Am. J. Reprod. Immunol. Microbiol. 9, 84–90PubMedGoogle Scholar
  6. Faulk, W.P. and McIntyre, J.A. (1983) Immunological studies of human trophoblast: Markers, subsets and functions. Immunol. Rev. 75, 139–175.PubMedCrossRefGoogle Scholar
  7. Faulk, W.P., Torry, D.S., and Mclntrye, J.A. (1986) Effects of serum versus plasma agglutination of antibody coated indicator cells by human rheumatoid factors. Clin. Immunol. Immunopath. (submitted).Google Scholar
  8. Faulk, W.P., Temple, A., Lovins, R., and Smith, N. (1978) Antigens of human trophoblast: a working hypothesis for their role in normal and abnormal pregnancies. Proc. Natl. Acad. Sci. USA 75, 1947–1951.PubMedCrossRefGoogle Scholar
  9. Garvey, J.S., Cremer, N.E., and Sussdorf, D.M. (1977) Methods in Immunology, 3rd edition, W.A. Benjamin, Inc., Readings, MA.Google Scholar
  10. McConnachie, P.R., Bloemers, J., Godtferson, D., Leming, G., Riseman, J., Finch, W.T., and Birtch, A.G. (1979) False positive 51Cr release tests during post transplant immunological monitoring due to recipient serum radioactive contaminations. J. Immunol. Methods 26, 197–201.PubMedCrossRefGoogle Scholar
  11. McConnachie, P.R. and McIntyre, J.A. (1984) Maternal antipaternal immunity in couples predisposed to repeated pregnancy losses. Am. J. Reprod. Immunol. 5, 145–150.PubMedCrossRefGoogle Scholar
  12. McIntyre, J.A. and Faulk, W.P. (1982) Allotypic trophoblast-lymphocyte cross-reactive (TLX) cell surface antigens. Human Immunol. 4, 27–35.CrossRefGoogle Scholar
  13. McIntyre, J.A. and Faulk, W.P. (1984) Histocompatibility and human pregnancy. Fertil. Steril. 41, 653–654.PubMedGoogle Scholar
  14. McIntyre, J.A. and Faulk, W.P. (1985) Laboratory and clinical aspects of research in chronic spontaneous abortion. Diagnost. Immunol. 3, 163–170.Google Scholar
  15. McIntyre, J.A. and Faulk, W.P. (1985) Antibody responses in secondary aborting women: effects of inhibitors in blood. Am. J. Reprod. Immunol. Microbiol. 9, 113–118.PubMedGoogle Scholar
  16. McIntyre, J.A., Faulk, W.P., Verhulst, S.J., and Colliver, J.A. (1983) Human trophoblast-lymphocyte cross-reactive (TLX) antigens define a new alloantigen system. Science 222, 1135–1137.PubMedCrossRefGoogle Scholar
  17. McIntyre, J.A., McConnachie, P.R., Taylor, C., and Faulk, W.P. (1984) Clinical, immunologic and genetic definitions of primary and secondary recurrent spontaneous abortions. Fertil. Steril. 42, 849–855.PubMedGoogle Scholar
  18. McIntyre, J.A., McConnachie, P.R., and Faulk, W.P. (1985) Characterization of maternal antipaternal antibodies in secondary aborting women. In: Contributions to Gynecology and Obstetrics, (ed.), V. Toder, Karger, Basel. Vol. 14. pp. 131–137.Google Scholar
  19. McIntyre, J.A., Faulk, W.P., Nichols-Johnson, V.R., and Taylor, C.G. (1986) Immunological testing and immunotherapy in recurrent spontaneous abortion. Obstet. Gynecol. 67, 169–175PubMedCrossRefGoogle Scholar
  20. Perlman, P. and Perlman, M. (1970) Contactual lysis of antibody coated chicken erythrocytes by purified lymphocytes. Cell Immunol. 1, 300–315.CrossRefGoogle Scholar
  21. Rogentine, G.N. and Plocinik, B.A. (1979) 51Cr release cytotoxicity techniques. NIAID Manual of Tissue Typing Techniques,p. 89.Google Scholar
  22. Skvaril, H.L. (1976) The question of specificity in binding human IgG subclasses to protein A-Sepharose. Immunochem. 13, 871–872.CrossRefGoogle Scholar
  23. Spieglberg, H.L., Perlman, H., and Perlman, P. (1976) Interaction of K lymphocyte with myeloma proteins of different IgG subclasses. J. Immunol. 116, 1464–1471.Google Scholar
  24. Taylor, C.G., Faulk, W.P., and McIntyre, J.A. (1985) Prevention of recurrent spontaneous abortions by leucocyte transfusions. J. Roy. Soc. Med. 78, 623–627.PubMedGoogle Scholar
  25. Torry, D.S., McConnachie, P.R., and McIntyre, J.A. (1985a) Complement-dependent (CDCE and CDC51Cr) and complement-independent (ADCC) antibodies in secondary aborting women. Am. J. Reprod. Immunol. Microbiol. 7, 135A.Google Scholar
  26. Torry, D.S., McIntyre, J.A., and McConnachie, P.R. (1985b) Characterization immunoglobulin class and subclass responses in secondary aborter sera. Reprod. Immunol.,in press.Google Scholar
  27. Torry, D.S., McIntyre, J.A., Faulk, W.P., and McConnachie, P.R. (1985c) In vitro control of secondary aborters’ antipaternal immunity. In: Immunology of Reproduction,Publishing House of the Bulgarian Academy of Sciences, Sophia. (in press).Google Scholar
  28. Torry, D.S., McIntyre, J.A., Faulk, W.P., and McConnachie, P.R. (1986) Inhibitors of complement-mediated cytotoxicity in normal and secondary aborter sera. Am. J. Reprod. Immunol. Microbiol. 10, 53–57.PubMedGoogle Scholar
  29. Wissloff, F., Michaelson, T.E., and Froland, S.S. (1974) Inhibition of antibody dependent human lymphocyte mediated cytotoxicity by Ig classes, Ig subclasses and Ig fragments. Scand. J. Immunol. 3, 29–38.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • Donald S. Torry
    • 1
  • John A. McIntyre
    • 1
    • 2
  • W. Page Faulk
    • 2
  • Peter R. McConnachie
    • 3
  1. 1.Department of Medical Microbiology and ImmunologySouthern Illinois University School of MedicineUSA
  2. 2.Methodist Center for Reproduction and Transplantation ImmunologyMethodist Hospital of IndianaIndianapolisUSA
  3. 3.Immuno Transplant LaboratoryMemorial Medical CenterSpringfieldUSA

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