The Preparation and Study of Detergent-Solubilized Receptors
A number of properties of cell surface receptors cannot be determined in sufficient detail without purification of the putative receptor and subsequent reconstitution of its biological function(s). For example, rigorous proof for the existence of receptor subtypes requires the study of receptor-ligand interactions in a purified preparation. Similarly, determining whether or not receptor affinity states are a manifestation of receptor interaction with components of an effector system is best accomplished by reconstituting isolated receptor and effector components into lipid vesicles of defined composition.
KeywordsAmmonium Sulfate Polyethylene Glycol Free Ligand Integral Protein Partial Specific Volume
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