Measurement of Enzyme Activity

  • Robert K. Scopes
Part of the Springer Advanced Texts in Chemistry book series (SATC)

Abstract

Most people approaching the problem of enzyme purification will be following a standard procedure for measuring enzyme activity. But in some cases, it may be useful to develop an alternative assay, for instance, a rapid spectrophotometry method to avoid queuing for the use of a scintillation counter. On rare occasions a newly discovered enzyme will need a new assay method. Ultimately the biochemist wants to know the relationship between an enzyme’s activity in vitro and its physiological situation. But the pH, ionic strength, and, particularly, the substrate concentration in vivo are likely to be quite different from the conditions used to measure the enzyme activity during a purification process. What is required here is a reliable, reproducible assay which will relate the amount of enzyme in one fraction with another, regardless of what the “activity” may mean in a physiological context.

Keywords

Pyruvate Kinase Methyl Viologen Continuous Method Coupling Enzyme Couple Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1982

Authors and Affiliations

  • Robert K. Scopes
    • 1
  1. 1.Department of BiochemistryLa Trobe UniversityBundooraAustralia

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