The Anatomy of Biological Interfaces

  • J. David Robertson

Abstract

It is generally recognized that biological membranes have a common, basic lipid bilayer structure(179,180,191,193,231) which contains external, internal, and transmembrane proteins with chemical asymmetry as a fundamental feature(215–217); the asymmetry involves both lipids and proteins (see Rothman and Lenard(201) for a recent review). Certain glycoproteins such as glycophorin in erythrocyte membranes(146,207) are arranged with a protein moiety in the internal leaflet connected to a glycoprotein moiety in the external leaflet.(241) In the case of glycophorin, the internal and external moieties are connected by a stretch of hydrophobic amino acid residues traversing the bilayer probably as an α helix.

Keywords

Lipid Bilayer Basic Protein Lipid Monolayer Unit Membrane Purple Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Abrams, A., C. Bavan, and H. P. Schnebli. 1972. The isolation of bacterial membrane ATPase and nectin. Methods Enzymol. 32: 428–446.CrossRefGoogle Scholar
  2. 2.
    Allen, J. M., and J. Gockerman. 1%4. Electrophoretic separation of multiple forms of particle associated acid phosphatase. Ann. N.Y. Acad. Sc. 121: 616–633.Google Scholar
  3. 3.
    Bach, D., and J. R. Miller. 1973. Interaction of bilayers with basic polypeptides. J. Membr. Biol. 11: 237–254.CrossRefGoogle Scholar
  4. 4.
    Barka, T. 1961. Studies of acid phosphatase I. Electrophoretic separation of acid phosphatases of rat liver on polyacrylamide gels. J. Histochem. Cytochem. 9: 542–433.PubMedCrossRefGoogle Scholar
  5. 5.
    Barr, L., M. M. Dewey, and W. Berger. 1965. Propagation of action potentials and the structure of the nexus in cardiac muscle. J. Gen. Physiol. 48: 797–823.PubMedCrossRefGoogle Scholar
  6. 6.
    Bencina, B., P. R. Carnegie, T. A. McPherson, and G. Robson. 1969. Encephalitogenic basic protein from sciatic nerve. FEBS Lett. 4: 9–12.PubMedCrossRefGoogle Scholar
  7. 7.
    Benedetti, E. D., and P. Emmelot. 1965. Electron microscopic observations on negatively stained plasma membranes isolated from rat liver. J. Cell Biol. 26: 299–304.PubMedCrossRefGoogle Scholar
  8. 8.
    Benedetti, E. L., and P. Emmelot. 1968. Hexagonal array of subunits in tight junctions separated from isolated rat liver plasma membranes. J. Cell Biol. 38: 15–24.PubMedCrossRefGoogle Scholar
  9. 9.
    Bennett, M. V. L. 1973. Function of electrotonic junctions in embryonic and adult tissues. Fed. Proc. 33: 65–75.Google Scholar
  10. 10.
    Bennett, M. V. L., and J. P. Trinkaus. 1970. Electrical coupling between embryonic cells by way of extra-cellular space and specialized junctions. J. Cell Biol. 44: 592–610.PubMedCrossRefGoogle Scholar
  11. 11.
    Benson, A. A. 1964. Plant membrane lipids. Annu. Rev. Plant Physiol. 15: 1–16.CrossRefGoogle Scholar
  12. 12.
    Benson, A. A. 1966. On the orientation of lipids in chloroplast and cell membranes. J. Am. Oil Chem. Soc. 43: 265–270.PubMedCrossRefGoogle Scholar
  13. 13.
    Blair, P. V., T. Oda, E. E. Green, and H. Fernandez-Moran. 1963. Studies on the electron transfer system. LIV. Isolation of the unit of electron transfer. Biochemistry 2: 756–764.PubMedCrossRefGoogle Scholar
  14. 14.
    Blasie, J. K. 1972. The location of photopigment molecules in the cross section of frog retinal receptor disk membranes. Biophys. J. 12: 191–204.PubMedCrossRefGoogle Scholar
  15. 15.
    Blasie, J. K., M. M. Dewey, and A. Blaurock, 1965. Electron microscope and low-angle X-ray diffraction studies on outer segment membranes from the retina of the frog. J. Mol. Biol. 14: 143–152.PubMedCrossRefGoogle Scholar
  16. 16.
    Blaurock, A. F. 1975. Bacteriorhodopsin: A trans-membrane pump containing a-helix. J. Mol. Biol. 93: 139–158.PubMedCrossRefGoogle Scholar
  17. 17.
    Blaurock, A. F. and W. Stoeckenius. 1971. Structure of the purple membrane. Nature (New Biol.) 233: 152155.Google Scholar
  18. 18.
    Blaurock, A. E., and M. H. F. Wilkins. 1969. Structure of frog photoreceptor membranes. Nature 223: 906–909.PubMedCrossRefGoogle Scholar
  19. 19.
    Blobel, G., and B. Dobberstein. 1975. Transfer of proteins across membranes. J. Cell Biol. 67: 835–851.PubMedCrossRefGoogle Scholar
  20. 20.
    Bloemendal, H., A. Zweers, F. Vermorken, I. Dunia, and E. L. Benedetti. 1972. The plasms membranes of eye lens fibers: Biochemical and structural characterization. Cell. Diff. 1: 91–106.CrossRefGoogle Scholar
  21. 21.
    Blodgett, K. D. 1935. Film built by depositing successsive monomolecular layers in a solid surface. J. Am. Chem. Soc. 57: 1007–1022.CrossRefGoogle Scholar
  22. 22.
    Blodgett, K. B., and I. Langmiur. 1937. Builtup films of barium stearate and their optical properties. Phys. Rev. 51: 964–982.CrossRefGoogle Scholar
  23. 23.
    Bloj, B., and D. B. Zilversmit. 1976. Asymmetry and trans position rates of phosphatidylcholine in rat erythrocyte ghosts. Biochemistry 15: 1277–1283.PubMedCrossRefGoogle Scholar
  24. 24.
    Branton, D. 1971. Freeze-etching studies of membrane structure. Philos. Trans. R. Soc. Lond. B 261: 133–138.CrossRefGoogle Scholar
  25. 25.
    Branton, D., and D. W. Deamer. 1972. Membrane structure. in: Protoplasmatologia. M. Alfert, H. Bauer, W. Sandritter, and P. Sitte, eds. Springer-Verlag, Berlin and New York.Google Scholar
  26. 26.
    Branton, D., and R. B. Park. 1967. Subunits in chloroplast lamellar. J. Ultrastruct. Res. 19: 283–303.PubMedCrossRefGoogle Scholar
  27. 27.
    Braun, V. 1975. Covalent lipoprotein from the outer membrane of Escherichia coli. Biochim. Biophys. Acta 415: 335–377.CrossRefGoogle Scholar
  28. 28.
    Bretscher, M. S. 1971. Human erythrocyte membranes: Specific labeling of surface proteins. J. Mol. Biol. 58: 775.PubMedCrossRefGoogle Scholar
  29. 28.
    Bretscher, M. S. 1972. Phosphatidylethanol amino. Differential labelling in intact cells and cell ghasts of human erythrocytes by a membrane impermeable reagent. J. Mol. Biol. 71: 523–528.PubMedCrossRefGoogle Scholar
  30. 29.
    Bretscher, M. S., 1973. Membrane structure: Some general principles. Science 181: 622–629.PubMedCrossRefGoogle Scholar
  31. 31.
    Brightman, M. W., and T. S. Reese. 1969. Junctions between intimately apposed cell membranes in the vertebrate brain. J. Cell Biol. 40: 648–677.PubMedCrossRefGoogle Scholar
  32. 32.
    Brown, J. C., and R. C. Hunt. 1976. Identification of a high molecular weight transmembrane protein in mouse L cells. In: Membranes and Neoplasia: New Approaches and Strategies. V. T. Marchesis, ed. Alan R. Liss, New York. pp. 179–188.Google Scholar
  33. 33.
    Bullivant, S., and A. Ames, III. 1966. A simple freeze-fracture replication method for electron microscopy. J. Cell Biol. 29: 435–447.Google Scholar
  34. 34.
    Cadenhead, D. A. 1970. Monolayers of synthetic phospholipids. Recent Progress in Surface Science 3: 169–192.Google Scholar
  35. 35.
    Caspar, D. L. D., D. A. Goodenough, L. Makowski, and W. C. Phillips 1977. Structural dynamics of the gap junction lattice. Neurosciences Research Program Symposium, April 6–8, 1975, Electronic Junctions. M. V. L. Bennett and D. A. Goodenough eds., Neurosciences Res. Progr. Bulletin,in press.Google Scholar
  36. 36.
    Caspar, D. L. D., and D. A. Kirschner. 1971. Myelin membrane structure at 10 A resolution. Nature (New Biol.) 231: 46–52.CrossRefGoogle Scholar
  37. 37.
    Chabre, M. 1975. X-ray diffraction studies of retinal rods. I. Structure of the disk membrane: Effect of Illumination. Biochim. Biophys. Acta 382: 322–335.PubMedCrossRefGoogle Scholar
  38. 38.
    Chabre, M., and A. Cavaggioni. 1973. Light induced changes of ionic flux in the retinal rod. Nature (New Biol.) 244: 118–120.Google Scholar
  39. 39.
    Chalcroft, J. P., and S. Bullivant. 1970. An intrepretation of liver cell membrane and junction structure based on observation of freeze fracture replicas of both sides of the fracture. J. Cell Biol. 47: 49–60.PubMedCrossRefGoogle Scholar
  40. 40.
    Changeaux, J. P. 1975. Lecture at International Conference on Biological Membranes, Crans sur Sierre, Switzerland, June 15–21. Google Scholar
  41. 41.
    Chapman, D., and H. Urbina. 1971. Phase transitions and bilayer structure of Mycoplasma laidlawii. B. FEBS Lett. 12: 169–172.CrossRefGoogle Scholar
  42. 42.
    Clark, A. W., and D. Branton. 1968. Fracture faces in frozen outer segments from the guinea pig retina. Z. Zellforsch. 91: 586–603.PubMedCrossRefGoogle Scholar
  43. 43.
    Colacicco, G. 1969. Lipid monolayers: Mechanisms of protein penetration with regard to membrane models. Lipids 5: 636–649.CrossRefGoogle Scholar
  44. 44.
    Cone, R. A. 1972. Rotational diffusion of rhodopsin in the visual receptor membrane. Nature (New Biol.) 236: 39–43.Google Scholar
  45. 45.
    Corless, J. M. 1971. X-ray diffraction studies on retinal rod photoreceptor membrane structure. Unpublished Ph.D. thesis, Duke University, Durham, North Carolina.Google Scholar
  46. 46.
    Corless, J. M. 1972. Lamellar structure of bleached and unbleached rod photoreceptor membranes. Nature 237: 229–231.PubMedCrossRefGoogle Scholar
  47. 47.
    Corless, J. M., W. H. Cobbs, III, M. J. Costello, and J. D. Robertson, 1975. On the asymmetry of frog retinal rod outer segment disk membranes: Interpretation of freeze-fracture data. Exp. Eye Res. 23(3): 295324.Google Scholar
  48. 48.
    Costello, M. J., and T. Gulik-Krzywicki. 1976. Correlated x-ray diffraction and freeze-fracture studies on membrane model systems. Biochim. Biophys. Acta 455: 412–432.PubMedCrossRefGoogle Scholar
  49. 49.
    Csejtey, J., J. F. Hallpike, C. W. M. Adams, and O.B. Bayliss. 1972. Histochemistry of myelin XIV peripheral nerve myelin proteins: Electrophoretic and histochemical correlations. J. Neurochem. 19: 19311935.Google Scholar
  50. 50.
    Danielli, J. F., and H. A. Dayson. 1935. A contribution to the theory of permeability of thin films. J. Cell. Comp. Physiol. 5: 495–508.CrossRefGoogle Scholar
  51. 51.
    da Silva, P., and D. Branton. 1970. Membrane splitting in freeze-etching: Covalently bound ferritin as a membrane marker. J. Cell Biol. 45: 598–605.CrossRefGoogle Scholar
  52. 52.
    Deamer, D. W. 1970. An alternative model for molecular organization in biological membranes. Bioenergetics 1: 237–246.CrossRefGoogle Scholar
  53. 53.
    Deamer, D. W., R. Leonard, A. Tardieu, and D. Branton. 1970. Lamellar and hexagon lipid phases visualized by freeze-etching. Biochim. Biophys. Acta 219: 47–60.PubMedCrossRefGoogle Scholar
  54. 54.
    Deamer, D. W., and N. Yamanaka. 1975. Freeze-fracture particles in protease treated membranes. Biophys. J. 15: 110–111.Google Scholar
  55. 55.
    Decker, R. S., and D. S. Friend. 1974. Assembly of gap junctions during amphibian neurulation. J. Cell Biol. 62: 32–47.PubMedCrossRefGoogle Scholar
  56. 56.
    Demel, R. A., Y. London, W. S. M. Geurts van-Kessel, F. G. A. Vossenberg, and L. L. M. vanDennen. 1973. The specific interaction of myelin basic protein with lipids at the air/water interface. Biochim. Biophys. Acta 311: 507–519.PubMedCrossRefGoogle Scholar
  57. 57.
    DePetris, S., and M. C. Raff. 1972. Distribution of immunoglobulin on the surface of mouse lymphoid cells as determined by immunoferritin electron microscopy. Antibody-induced, temperature dependent redistribution and its implications for membrane structure. Eur. J. Immunol. 2: 523, 540.Google Scholar
  58. 58.
    Dervichian, D. G. 1954. The surface properties of fatty acids and allied substances. In: Progress in the Chemistry of Fats and Other Lipids, Vol. 2. Holman, R. T., Lundberg, W. O., and Malkin, T., eds. Academic Press, New York. p. 193.Google Scholar
  59. 59.
    Dewey, M. M., and L. Barr. 1962. Intercellular connection between smooth muscle cells: The nexus. Science 137: 670–672.PubMedCrossRefGoogle Scholar
  60. 60.
    Dewey, M. M., and L. Barr. 1964. A study of the structure and distribution of the nexus. J. Cell Biol. 23: 553–585.PubMedCrossRefGoogle Scholar
  61. 61.
    Dickenson, J. P., K. M. Jones, S. R. Asparicio, and C. E. Lumsden. 1970. Localization of encephalitogenic protein in the intraperiod line of lamellar myelin. Nature 227: 1133–1134.CrossRefGoogle Scholar
  62. 62.
    Dunia, I., K. Sen, E. L. Benedetti, A. Zweers, and H. Bloemendal. 1974. Isolation and protein patterns of eye lens fiber junctions. FEBS Lett. 45: 139–144.PubMedCrossRefGoogle Scholar
  63. 63.
    Elgsaeter, A., and D. Branton. 1974. Intramembrane particle aggregation in erythrocyte ghasts. I. The effects of protein removal. J. Cell Biol. 63: 1018–1030.PubMedCrossRefGoogle Scholar
  64. 64.
    Eng, L. F. 1971. Molecular weights of the major myelin proteins. Fed. Proc. 30: 1248 (Abstr.).Google Scholar
  65. 65.
    Eugelman, D. M. 1971. Lipid bilayer structure in the membrane of Mycoplasma laidlawii. J. Mol. Biol. 58: 153–165.CrossRefGoogle Scholar
  66. 66.
    Evans, E. A. 1973. New membrane concept applied to the analysis of fluid shear and micropipette-deformed red blood cells. Biophys. J. 13: 941.PubMedCrossRefGoogle Scholar
  67. 67.
    Everly, J. L., R. O. Brady, and R. H. Quarles. 1973. Evidence that the major protein in rat sciatic nerve myelin is a glycoprotien. J. Neurochem. 21: 329–334.PubMedCrossRefGoogle Scholar
  68. 68.
    Eylar, E. H. 1970. Amino acid sequence of the basic protein of the myelin membrane. Proc. Natl. Acad. Sci. U.S.A. 67: 1425–1431.PubMedCrossRefGoogle Scholar
  69. 69.
    Eylar, E. H., J. Salk, G. C. Beveridge, and L. V. Braun. 1969. Experimental allergic encephalomyelitis, an encephalitogenic basic protein from bovine myelin. Arch. Biochem. Biophys. 132: 34–48.PubMedCrossRefGoogle Scholar
  70. 70.
    Eylar, E. H., and M. Thompson. 1969. Allergic en 90. cephalomyelitis: The physico-chemical properties of the basic protein encephalitogen from bovine spinal cord. Arch. Biochem. Biophys. 129: 468–479.PubMedCrossRefGoogle Scholar
  71. 70a.
    Eylar, E. N. 1977. The myelin membrane and its basic proteins. In: Structure of Biological Membranes. S. Abrahamsson and I. Pascher, eds. Plenum Press, New York. pp. 157–176.CrossRefGoogle Scholar
  72. 71.
    Farquhar, M. G., and G. E. Palade. 1964. Functional organization of amphibian skin. Proc. Natl. Acad.. Sci. U.S.A. 51: 569–577.PubMedCrossRefGoogle Scholar
  73. 72.
    Farquhar, M. G., and G. E. Palade. 1965. Cell junctions in amphibian skin. J. Cell Biol. 26: 263–291.PubMedCrossRefGoogle Scholar
  74. 73.
    Finean, J. B. 1969. Biophysical contributions to membrane structure. Q. Rev. Biophys. 21: 1–23.CrossRefGoogle Scholar
  75. 74.
    Finean, J. B., R. Coleman, W. G. Green, and A. R. 94. Limbrick. 1966. Low angle x-ray diffraction and electron miscroscope studies of isolated cell membranes. J. Cell Sci. 2: 287–296.Google Scholar
  76. 75.
    Finean, J. B., R. Coleman, S. Knutton, and A. R. Limbrick. 1968. Structural studies of cell membrane preparations. J. Gen. Physiol. 51: 19s - 25s.PubMedGoogle Scholar
  77. 76.
    Fisher, K. A. 1975. “Half” membrane enrichment: Verification by electron microscopy. Science 190:983–984.Google Scholar
  78. 77.
    Fisher, K. A. 1976. Autoradiography of membrane “halves” 3H-cholesterol labeled erythrocytes. J. Cell Biol. 70: 218a.Google Scholar
  79. 78.
    Fletcher, W. F., and J. D. Robertson. 1975. Assembly of an “enclosed gap junction” by granulosa cells in the developing ovarian follicles of sexually immature rats. J. Cell Biol. 67: 116a.Google Scholar
  80. 79.
    Folch, J., and M. Lees. 1951. Proteolipids, a new type of tissue lipoproteins. J. Biol. Chem. 191: 807–817.PubMedGoogle Scholar
  81. 80.
    Folch-Pi, J., and P. J. Stoffyn. 1972. Proteolipids from membrane systems. Ann. N.Y. Acad. Sci. 195: 86107.CrossRefGoogle Scholar
  82. 81.
    Frye, L. D., and M. Edidin. 1970. The rapid intermixing of cell surface antigens after formation of mouse- 100. human heterokaryons. J. Cell Sci. 7: 319–335.PubMedGoogle Scholar
  83. 82.
    Furshpan, E. J., and T. Furakawa. 1962. Intracellular and extracellular responses of the several regions of the Mauthner cell of the goldfish. J. Neurophysiol. 101. 25: 732.Google Scholar
  84. 83.
    Furshpan, E. J., andD. D. Potter. 1959. Transmission at the giant motor synapses of the crayfish. J. Physiol. 145: 289–325.PubMedGoogle Scholar
  85. Gaines, G. L. 1966. In: Insoluble Monolayers at Liquid-Gas Interfaces. Wiley, New York.Google Scholar
  86. 85.
    Giannoni, G., F. J. Padden, and R. S. Rue. 1971. A lamellar complex of lecithin and poly-L-tyrosine. Biophys. J. 11: 1018–1029.PubMedCrossRefGoogle Scholar
  87. 86.
    Gilula, N. B. 1974. Junctions between cells. In: Cell Communication. R. P. Cox, ed. Wiley, New York, pp. 1–29.Google Scholar
  88. 87.
    Gilula, N. B. 1974. Isolation of rat liver gap junctions and characteristics of the polypeptides. J. Cell Biol. 63 (2): 111a.Google Scholar
  89. 88.
    Goodenough, D. A., and W. Stoekenius. 1972. The isolation of mouse hepatocyte gap junctions: Preliminary chemical characterization and x-ray diffraction. J. Cell Biol. 54: 646–656.PubMedCrossRefGoogle Scholar
  90. 89.
    Goodenough, D. A. 1974. Bulk isolation of mouse hepatocyte gap junctions: Characterization of the principal protein, connexin. J. Cell Biol. 61: 557–563.PubMedCrossRefGoogle Scholar
  91. 90.
    Goodenough, D. A. 1975. Correlated x-ray electron microscopic and biochemical studies of gap junctions. Presentation at Neurosciences Research Program work session on gap junctions, Boston, Mass., April 7–8, 1975.Google Scholar
  92. 91.
    Goodenough, D. A. 1976. Channels transversing two junctional membranes and invervening gap. J. Cell Biol. 71: 334–335.Google Scholar
  93. 92.
    Goodenough, D. A. 1976. In vitro formation of gap junction vesicles. J. Cell Biol. 71: 220–231.Google Scholar
  94. 93.
    Goodenough, V. W., and L. A. Staehelin. 1971. Structural differentiation of stacked and unstacked chloroplast membranes: Freeze-etch electron microscopy of wild-type and mutant strains of Chlamydo-monas. J. Cell Biol. 48: 594–619.CrossRefGoogle Scholar
  95. 94.
    Gordesky, S. E., and G. V. Marinetti. 1973. The asymmetric arrangement of PL’s in human erythrocyte membranes. Biochem. Biophys. Res. Commun. 50: 1027–1031.PubMedCrossRefGoogle Scholar
  96. 95.
    Gorter, E., and R. Grendel. 1925. On bimolecular layers of lipoid on the chromocytes of the blood. J. Exp. Med. 41: 439–443.PubMedCrossRefGoogle Scholar
  97. 96.
    Gould, R. M., and Y. London. 1972. Specific interaction of central nervous system myelin basic protein with lipids. Effects of basic protein on glucose leakage from liposomes. Biochim. Biophys. Acta 290: 200–218.PubMedCrossRefGoogle Scholar
  98. 97.
    Green, J. B., M. C. Phillips, and G. G. Shipley. 1973. Structural investigations of lipid, polypeptide and protein multilayers. Biochim. Biophys. Acta 330: 243–253.PubMedCrossRefGoogle Scholar
  99. 98.
    Greenfield, S., S. Brostoff, E. H. Eylar, and P. Mo-rell. 1972. Protein composition of myelin of the peripheral nervous system. J. Neurochem. 20: 1207–1216.CrossRefGoogle Scholar
  100. 99.
    Gras, W. J., and C. R. Worthington. 1969. X-ray analysis of retinal photoreceptors. Proc. Natl. Acad. Sci. U.S.A. 63: 233–238.PubMedCrossRefGoogle Scholar
  101. 100.
    Gruener, W., and R. G. Peterson,. 1977. Positional relationships of peripheral nervous system myelin proteins. Texas Soc. Electron Microsc. Newslett. 8: 35–36.Google Scholar
  102. 101.
    Gulick-Krzywicki, T. 1974. Structural studies of the associations between biological membrane components. Biochim. Biophys. Acta 451: 1–28.Google Scholar
  103. 102.
    Hall, J. E., and R. Latorre. 1976. Nonactin-K+ Complex as a probe for membrane asymmetry. Biophys. J. 15: 99.CrossRefGoogle Scholar
  104. 103.
    Hanai, T., D. A. Haydon, and W. R. Redwood. 1966. The water permeability of artificial biomolecular leaflets: A comparison of radio-tracer and osmotic methods. Ann. N.Y. Acad. Sci. 137: 731–744.PubMedCrossRefGoogle Scholar
  105. 104.
    Harkins, W. D., E. C. H. Davies, and F. L. Clark. 1917. The orientation of molecules in the surfaces of liquids, the energy relations at surfaces, solubility, absorption, emulsification, molecular association, and the effect of acids and bases on interfacial tension (surface energy VI). J. Am. Chem. Soc. 39: 541–596.CrossRefGoogle Scholar
  106. 105.
    Henderson, R. 1975. The structure of the purple membrane for Halobacterium halobium: Analysis of the x-ray diffraction pattern. J. Mol. Biol. 93: 123–138.PubMedCrossRefGoogle Scholar
  107. 106.
    Henderson, R., and P. N. T. Unwin. 1975. Three-dimensional model of purple membrane obtained by electron microscopy. Nature 257: 28–32.PubMedCrossRefGoogle Scholar
  108. 107.
    Hendler, R. W. 1971. Biological membrane ultrastructure. Physiol. Rev. 51: 66–97.PubMedGoogle Scholar
  109. 108.
    Heuser, J. E., T. S. Reese, and M. D. Landis. 1974. Functional changes in frog neuromuscular junctions studied with freeze-fracture. J. Neurocytol. 3: 109131Google Scholar
  110. 109.
    Hicks, R. M.. 1965. The fine structure of the transitional epithelium of rat ureter. J. Cell Biol. 26: 25–48.PubMedCrossRefGoogle Scholar
  111. 110.
    Hicks, R. M. 1966. The function of the Golgi complex in transitional epithelium. Synthesis of the thick cell membrane. J. Cell Biol. 30: 623–643.PubMedCrossRefGoogle Scholar
  112. 111.
    Hicks, R. M., and B. Ketterer. 1969. Hexagonal lattice of subunits in the thick luminal membrane of the rat urinary bladder. Nature 224: 1304–1305.PubMedCrossRefGoogle Scholar
  113. 112.
    Hicks, R. M., and B. Ketterer. 1970. Isolation of the plasma membrane of the luminal surface of the rat bladder epithelium and the occurrence of a hexagonal lattice of subunits both in negatively stained whole mounts and in sectioned membranes. J. Cell Biol. 45: 542–553.PubMedCrossRefGoogle Scholar
  114. 113.
    Hagins, W. A., W. E. Robinson, and S. Yoshikami. 1975. Ionic aspects of excitation in cord outer segments. In: Energy Transformation in Biological Systems. Ciba Foundation Symposium 31, New Series. American Elsevier, New York. pp. 169–189.Google Scholar
  115. 114.
    Hong, K., and W. L. Hubbell. 1972. Preparation and properties of phospholipid bilayers containing rhodopsin. Proc. Natl. Acad. Sci. U.S.A. 69: 2617–2621.PubMedCrossRefGoogle Scholar
  116. 115.
    Hubbell, W. L., and H. M. McConnell. 1968. Spin-label studies of the excitable membranes of nerve and muscle. Proc. Natl. Acad. Sci. U.S.A. 61: 12–16.PubMedCrossRefGoogle Scholar
  117. 116.
    Ito, S., E. Sato, and W. R. Lowenstein. 1975. Studies on the formation of a permeable cell membrane. II Evolving junctional conductance and junctional insulation. J. Membr. Biol. 19: 338–356.Google Scholar
  118. 117.
    Ito, S., E. Sato, and W. R. Lowenstein. 1975. Studies on the formation of a permeable cell membrane junction under various conditions of membrane contact. I Effects of colchicine, cytochalasin B, dinitrophenol. J. Membr. Biol. 19: 305–338.Google Scholar
  119. 118.
    Jakoi, E. R., and J. D. Robertson. 1975. Further characterization of a plasma membrane protein, ligatin, essential for the attachment of n-acetyl glucosaminidase. J. Cell Biol. 67: 190a.Google Scholar
  120. 119.
    Jakoi, E. R., G. Zampighi, and J. D. Robertson. 1975. Isolation and characterization of a water-soluble membrane protein from suckling rat ileum. Biophys. J. 15: 218a.Google Scholar
  121. 120.
    Jakoi, E. R., G. Zampighi, and J. D. Robertson. 1975. Regular structures in membranes. II. Morphological and biochemical characterization of two water-soluble membrane proteins isolated from the suckling rat ileum. J. Cell Biol. 70: 97–111.CrossRefGoogle Scholar
  122. 121.
    Jakoi, E. R., G. Zampighi, and J. D. Robertson. 1975. Morphological and biochemical characterization of two water-soluble membrane proteins isolated from the suckling rat ileum. In: Proceeding of the International Conference on Biological Membranes, Crans sur Sierre Switzerland, June 10–15. L. Bolis, J. Hoffman, and A. Leaf, eds. Raven Press, New York. pp. 263–272.Google Scholar
  123. 122.
    Johnson, R., M. Hammer, J. Sheridan, and J. P. Revel. 1974. Gap junction formation between reaggregated Novikoff hepatoma cells. Proc. Natl. Acad. Sci. U.S.A. 71: 4536–4540.PubMedCrossRefGoogle Scholar
  124. 123.
    Jost, P. C., R. A. Capaldi, G. Vanderkooi, and O. H. Griffith. 1973. Lipid-protein and lipid-lipid interactions in cytochrome oxidase model membranes. J. Supramol. Struct. 1: 269–280.PubMedCrossRefGoogle Scholar
  125. 124.
    Ketterer, B., R. M. Hicks, L. Christodoulides, and D. Beale. 1973. Studies of the chemistry of the luminal plasa membrane of rat bladder epithelial cells. Biochim. Biophys. Acta 311: 180–190.PubMedCrossRefGoogle Scholar
  126. 125.
    Kirk, R. G., and M. Ginzburg. 1972. Ultrastructure of two species of Halobacterium. J. Ultrastruct. Res. 41: 80–94.CrossRefGoogle Scholar
  127. 126.
    Kirk, R. G., and D. C. Tosteson. 1973. Cation transport and membrane morphology. J. Membr. Biol. 12: 273–285.PubMedCrossRefGoogle Scholar
  128. 127.
    Knutton, S., A. R. Limbrick, and J. D. Robertson. 1974. Regular structure in membranes. I. Membranes in the endocytic complex of ileal epithelial cells. J. Cell Biol. 62: 679–694.PubMedCrossRefGoogle Scholar
  129. 128.
    Knutton, S., and J. D. Robertson. 1976. Regular structures in membranes: The luminal plasma membrane of the cow urinary bladder. J. Cell Sci. 22: 355370.Google Scholar
  130. 129.
    Korn, E. D. 1966. Structure of biological membranes. Science 153: 1491–1498.PubMedCrossRefGoogle Scholar
  131. 130.
    Kornberg, R. D., and H. M. McConnell. 1971. Inside and outside transitions of phospholipids in vesicle membranes. Biochemistry 10: 1111–1120.PubMedCrossRefGoogle Scholar
  132. 131.
    Kornberg, R. D., and H. M. McConnell. 1971. Lateral diffusion of phospholipids in a vesicle membrane. Proc. Natl. Acad. Sci. U.S.A. 88(10): 2564–2568.Google Scholar
  133. 132.
    Laatsch, R. H., M. W. Kies, S. Gordon, and E. C. Alvord. 1962. The encephalomyelitic activity of myelin isolated by ultracentrifugation. J. Exp. Med. 115: 777–778.PubMedCrossRefGoogle Scholar
  134. 133.
    La Celle, P. 1975. Elastic behavior of spectrin-phospholipid membranes. Biophys. J. 15: 210A.Google Scholar
  135. 134.
    Langmuir, J. 1917 The constitution and fundamental properties of solids and liquids II. Liquids. J. Am.Chem. Soc. 37: 1848–1905.CrossRefGoogle Scholar
  136. 135.
    Larsson, K. 1973. Lipid multilayers. In: Surface and Colliod Science, Vol. 6. Matijevic, E., and Eirich, F. R., eds. Wiley, New York. p. 261.Google Scholar
  137. 136.
    Lenard, J., and S. J. Singer. 1966. Protein conformation in cell membrane preparations as studied by optical rotatory dispersion and circular dischroism. Proc.Natl. Acad. Sci. U.S.A. 56: 1828–1835.PubMedCrossRefGoogle Scholar
  138. 137.
    Limbrick, A. R., and J. B. Finean. 1970. X-ray diffraction and electron microscopic studies of the brush border membrane of guinea pig intestinal epithelial cells. J. Cell Sci. 7: 373–386.PubMedGoogle Scholar
  139. 138.
    Limbrick, A. R., and S. Knutton. 1975. The perturbation of the human erythrocyte membrane by phospholipase C. J. Cell Sci. 19: 341–355.PubMedGoogle Scholar
  140. 139.
    London, Y., R. O. Demel, W. S. M. Geurts von Kessel, P. Fahler, and I. Van Deener. 1974. The interaction of the “Folch-Lees” protein with lipids at the air/water interface. Biochim. Biophys. Acta 382: 69–84.Google Scholar
  141. 140.
    London, Y., and F. G. A. Vossenberg. 1973. Specific interaction of central nervous system myelin basic protein with lipids. Specific regions of the protein sequence protected from the proteolytic action of trypsin. Biochim. Biophys. Acta 478: 478–490.Google Scholar
  142. 141.
    Lowenstein, W. 1973. Membrane junctions in growth and differentiation. Fed. Proc. 30: 60–64.Google Scholar
  143. 142.
    Luzzati, V., and F. Husson. 1962. The structure of the liquid-crystalline phases of lipid-water systems. J. Cell Biol. 12: 207–219.PubMedCrossRefGoogle Scholar
  144. 143.
    MacLennan, D. H., and J. Asai. 1968. Studies on the mitochondrial adenosine triphosphatase system V. Localization of the oligomycin sensitivity conferring protein. Biochem. Biophys. Res. Commun. 33: 441447.Google Scholar
  145. 144.
    Malcolm, B. R. 1973. The structure and properties of monolayers of synthetic polypeptides at the air-water interface. In: Prog. Surf Membr. Sci. 7: 183–229.Google Scholar
  146. 145.
    Marchesi, V. T., and E. Steers. 1968. Selective solubilization of a protein component of red cell membrane. Science 159: 203–204.PubMedCrossRefGoogle Scholar
  147. 146.
    Marchesi, V. T., T. W. Tillack, R. L. Jackson, J. P. Segrest, and R. E. Scott. 1972. Chemical characterization and surface orientation of the major glycoprotein of the human erythrocyte membrane. Proc. Natl. Acad. Sci. U.S.A. 69:1445-1449.Google Scholar
  148. 147.
    Mateu, L., V. Luzzati, Y. London, R. M. Gould, F. G. A. Vosseberg, and J. Olive. 1973. X-ray diffraction and electron microscope study of the interactions of myelin components. The structure of a lamellar phase with a 150 to 180 A repeat distance containing basic proteins and acidic lipids. J. Mol. Biol. 75: 697–709.PubMedCrossRefGoogle Scholar
  149. 148.
    Mathews, F. S., P. Argos, and M. Levine. 1971. The structure of cytochrome b5 at 2.0 A resolution. Symp. Quant. Biol. 36: 387–395.CrossRefGoogle Scholar
  150. 149.
    McIntosh, T. J., R. C. Waldbillig, and J. D. Robertson. 1976. Lipid bilayer ultrasturcture. Electron density profiles and chain tilt angles as determined by x-ray diffraction. Biochim. Biophys. Acta 448: 15–33.PubMedCrossRefGoogle Scholar
  151. 150.
    McNamee, M. G., and H. M. McConnell. 1973. Transmembrane particles and PL flip-flop in excitable membrane vesicles. Biochemistry 12: 2951–2958.PubMedCrossRefGoogle Scholar
  152. 151.
    McNutt, N. S., and R. S. Weinstein,. 1973. Membrane ultrastructure at mammalian intercellular junctions. In: Progress in Biophysics and Molecular Biology. J. A. V. Butler and D. Nobl, eds. Pergamon, Oxford. pp. 45–101.Google Scholar
  153. 152.
    Miller, I. R., and D. Bach. 1974. Composition of bilayers and monolayers and absorption of basic polypeptides onto lipid surface layers. J. Colloid Interface Sci. 49: 453–461.CrossRefGoogle Scholar
  154. 153.
    Montai, M., and P. Mueller. 1972. Formation of bimolecular membranes from lipid monolayers and a study of their electrical properties. Proc. Natl. Acad. Sci. 69: 3561–3566.CrossRefGoogle Scholar
  155. 154.
    Moor, H., K. Muhlethaler, H. Waldner, and A. FreyWyssling. 1961. A new freezing ultramicrotome. J.Cell Biol. 10: 1–15.CrossRefGoogle Scholar
  156. 155.
    Mueller, P., D. V. Rudin, M. Glen, and W. C. Wescott. 1962. Reconstitution of excitable cell membrane structure in vitro. ciruculation XXVI:1167–1171.Google Scholar
  157. 156.
    Nakao, A., W. J. Osmin, and E. R. Esitein. 1966. Basic proteins from the acidic extract of bovine spinal cord. I. Isolation and characterization. Biochim. Biophys. Acta 130: 163–170.CrossRefGoogle Scholar
  158. 157.
    Ornstein, L., and B. Davis. 1962. Disk Electrophoresis, Parts I and I I. Distillation Products Industries, Rochester, New York.Google Scholar
  159. 158.
    Ottolenghi, A. 1973. Preparation and characterization of mouse intestinal phospholipase. Lipids 8: 415–425.PubMedCrossRefGoogle Scholar
  160. 159.
    Ornstein, L. 1957. Osmiophilia fact or fiction. J. Biophys. Biochem. Cytol. 3: 809–811.PubMedCrossRefGoogle Scholar
  161. 160.
    Papahadjopoulos, D. 1968. Surface properties of acidic phospholipids: Interaction of monolayer of hydrated liquid crystals with uni-and bivalent metal ions. Biochim. Biophys. Acta 163: 240–254.PubMedCrossRefGoogle Scholar
  162. 161.
    Papahadjopoulos, D., W. J. Vail, and M. Moscarollo. 1975. Interaction of a purified protein from myelin with phospholipid membranes: Studies on the ultra-structure, phase transitions and permeability. J. Membr. Biol. 22: 143–164.PubMedCrossRefGoogle Scholar
  163. 162.
    Pappas, G. D., and M. V. L. Bennett. 1966. Specialized junctions involved in electrical transmission between neurons. Ann. N.Y. Acad. Sci. 137: 495–508.PubMedCrossRefGoogle Scholar
  164. 163.
    Parsons, D. S. 1963. Negative staining of thinly spread cells and associated virus. J. Cell Biol. 16: 620626.Google Scholar
  165. 164.
    Peterson, R. G. 1976. Myelin protein changes with digestion of whole sciatic nerve in trypsin. Life Sci. 18: 845–850.PubMedCrossRefGoogle Scholar
  166. 165.
    Poduslo, J. F., and P. E. Braun. 1975. Topographical arrangement of membrane proteins in the intact myelin sheath. J. Biol. Chem. 250: 1099–1105.PubMedGoogle Scholar
  167. 166.
    Racker, E. 1972. Reconstitution of oxidative phosphorylation and vesicles with respiratory control. In: Membrane Research, First ICN-UCLA Symposium on Molecular Biology. C. F. Fox, ed. Academic Press, New York. pp. 97–114.Google Scholar
  168. 167.
    Racker, E., and W. Stoeckenius, W. 1974. Reconstitution of purple membrane vesicles catalyzing light-driven proton uptake triphosphate formation. J. Biol.Chem. 249: 662–663.Google Scholar
  169. 168.
    Racker, E., D. D. Tyler, R. W. Estabrook, T. E. Conover, D. F. Parsons, and B. Chance. 1965. Camelations between electron-transport activity, DTPase and morphology of submitochondrial particles. In: Oxidases and Related Redox Systems, Vol II. T. E., King, H. S. Mason, and M. Morrison, eds. Wiley, New York. pp. 1077–1101.Google Scholar
  170. 169.
    Ranck, J. L., L. Mateu, D. M. Sadler, G. Tandien, T. Gulik-Krzywicki, and V. Luzzati. 1974. Order and disorders conformational transitions of the hydrocarbon chains of lipids. J. Mol. Biol. 85: 277–289.CrossRefGoogle Scholar
  171. 170.
    Rand, R. P., and V. Luzzati. 1968. X-ray diffraction study in water of lipids extracted from human erythrocytes, the position of cholesterol in the lipid lamellae. Biophys. J. 8: 125–137.PubMedCrossRefGoogle Scholar
  172. 171.
    Rash, J. E., and M. H. Ellismam. 1974. Studies of excitable membrane I. Macromolecular specialization of the neuromuscular junction and the nonjunctional sarcolemma. J. Cell Biol. 63: 568–586.CrossRefGoogle Scholar
  173. 172.
    Renooij, W., L. M. G. Van Golde, R. F. A. Zwaal, and L. L. M. Van Deenen. 1976. Topological asymmetry of phospholipid metabolism in rat erythrocyte membranes. J. Biochem. 61: 53–58.Google Scholar
  174. 173.
    Revel, J. P., and M. J. Karnovsky. 1967. Hexagonal array of subunits in intercellular junctions of the mouse heart and liver. J. Cell Biol. 33: C7 - C12.PubMedCrossRefGoogle Scholar
  175. 174.
    Reynolds, J. A., and C. Tanford. 1970. Binding of dodecyl sulfate to proteins at high binding ratios. Possible implications for state of proteins in biological membranes. Proc. Natl. Acad. Sci. U.S.A. 66: 10021007.Google Scholar
  176. 175.
    Robertson, J. D. 1953. Ultrastructure of two invertebrate synapses. Proc. Soc. Exp. Biol. Med. 82: 219223.Google Scholar
  177. 176.
    Robertson, J. D. 1958. Structural alterations in nerve fibers produced by hypotonic and hypertonic solutions. J. Biophys. Biochem. Cytol. 4: 349–364.PubMedCrossRefGoogle Scholar
  178. 177.
    Robertson, J. D. 1958. The ultrastructure of SchmidtLantermann clefts and related shearing defects of the myelin sheath. J. Biophys. Biochem. Cytol. 4: 39–46.PubMedCrossRefGoogle Scholar
  179. 178.
    Robertson, J. D. 1959. Preliminary observations on the ultrastructure of nodes of Ranvier. Z. Zellforsch. Mikrosk. Anat. 50: 553–560.CrossRefGoogle Scholar
  180. 179.
    Robertson, J. D. 1959. The ultrastructure of cell membranes and their derivatives. Biochem. Soc. Symp. 16: 3–43.PubMedGoogle Scholar
  181. 180.
    Robertson, J. D. 1960. The molecular biology of cell membranes. In: Molecular Biology. D. Nachmansohn, ed. Academic Press, New York. pp. 87–151.Google Scholar
  182. 181.
    Robertson, J. D. 1960. The molecular structure and contact relationships of cell membranes. In: Progress in Biophysics. B. Katz and J. A. V. Butler, eds. Pergamon, Oxford. pp. 343–418.Google Scholar
  183. 182.
    Robertson, J. D. 1961. Ultrastructure of excitable membranes and the crayfish median giant synapse. Ann. N.Y. Acad. Sci. 94: 339–389.PubMedCrossRefGoogle Scholar
  184. 183.
    Robertson, J. D. 1961. The unit membrane. In: Electron Microscopy in Anatomy, Proceedings of a Symposium held by the Anatomical Society of Great Britain on the Ultrastructure of Cells. J. D. Boyd, F. R. Johnson, and J. D. Lever, eds. Arnold, London, pp. 74–99.Google Scholar
  185. 184.
    Robertson, J. D., E. R. Jakoi, and G. Zampighi. 1975. Fine structure of the apical endocytic complex from suckling rat ileum. Anat. Rec. 181: 384.Google Scholar
  186. 185.
    Robertson, J. D., S. Knutton, A. R. Limbrick, E. R. Jakoi, and G. Zampighi. 1976. Regular structures in unit membranes. III. Further observatons on the particulate component of the suckling rat ileum endocytic membrane complex. J. Cell Biol. 70: 112–122.PubMedCrossRefGoogle Scholar
  187. 186.
    Robertson, J. D. 1963. The occurrence of a subunit of pattern in the unit membranes of club endings in Mauthner cell synapses in goldfish brains. J. Cell Biol. 19: 201–222.PubMedCrossRefGoogle Scholar
  188. 187.
    Robertson, J. D., T. S. Bodenheimer, and D. E. Stage. 1963. The ultrastructure of Mauthner cell synapses and nodes in goldfish brains. J. Cell Biol. 19: 159–199.PubMedCrossRefGoogle Scholar
  189. 188.
    Robertson, J. D. 1966. Current problems in unit membrane structure and contact relations. In: Nerve as a Tissue. K. Rodahl, ed. Harper, New York. pp. 11–48.Google Scholar
  190. 189.
    Robertson, J. D. 1966. Design principles of the unit membrane. In: Principles of Biomolecular Organization. G. E. W. Wolstenholme and M. O’Conner, eds. Ciba Foundation, Churchill, London. pp. 357–408.Google Scholar
  191. 190.
    Robertson, J. D. 1966. Granulo-fibrillar and globular substructure in unit membranes. Ann. N.Y. Acad. Sci. 137: 421–440.PubMedCrossRefGoogle Scholar
  192. 191.
    Robertson, J. D. 1972. The structure of biological membranes, current status Arch. Intern. Med. 129: 202–228.PubMedCrossRefGoogle Scholar
  193. 192.
    Robertson, J. D., and M. J. Costello. 1974. Electron microscopic and X-ray diffraction studies on membrane model systems. Proceedings of the 8th International Conference on Electron Microscopy, Canberra, Australia, August 25–31, 1974. Electron Microscopy, Vol. II: Biologica, 218–219a. Aust. Acad. Sci., Canberra.Google Scholar
  194. 193.
    Robertson, J. D. 1975. Membrane models: The theoretical and real. In: The Nervous System, Vol. I: Basic Neurosciences. D. B. Tower, ed. Raven Press, New York. pp. 43–58.Google Scholar
  195. 194.
    Roelofsen, B., R. F. A. Zwaal, P. Comfurius, C. B. Woodward, and L. L. M. Van Deenen. 1971. Action of pure phospholipase Az and phospholipase C on human erythrocytes and ghosts. Biochim. Biophys. Acta 241: 925–929.PubMedCrossRefGoogle Scholar
  196. 195.
    Röhlich, P. 1975. Membrane-associated actin filaments in the cortical cytoplasm of the rat mast cell. Exp. Cell Res. 93: 293–298.PubMedCrossRefGoogle Scholar
  197. 196.
    Rollestan, F. S. 1974. Membrane bound and free ribosomes. Subcell. Biochem. 3: 91–117.Google Scholar
  198. 197.
    Romeo, D., A. Girard, and G. Rothfield. 1970. Reconstitution of a functional membrane enzyme system in a monomolecular film. I. Formation of a mixed mono-layer of lipopolysaccharide and phospholipid. J. Mol. Biol. 53: 475–490.PubMedCrossRefGoogle Scholar
  199. 198.
    Romeo, D., A. Hinckley, and L. Rothfield. 1970. Reconstitution of a functional membrane enzyme system in a monomolecular film II. Formation of a functional ternary film of lipopolysaccharide, phospholipid and transferase enzyme. J. Mol. Biol. 53: 491–501.PubMedCrossRefGoogle Scholar
  200. 199.
    Rosenbluth, J. Z., T. E. Ukena, H. H. Yin, R. D. Berlin, and M. J. Karnovsky. 1975. A comparative evaluation of the redistribution of concoanavalin A binding sites on the surfaces of normal, virally-transformed and protease-treated fibroblasts. Proc. Natl. Acad. Sci. U.S.A. 70:1625–1629; Yahara, I., and G. M. Edelman. 1975. Electron microscopic analysis of the modulation of lymphocyte receptor mobility. Exp. Cell Res. 91: 125–142.CrossRefGoogle Scholar
  201. 200.
    Rothman, J. E., and E. P. Kennedy. 1977. Asymmetrical distribution of phospholipids in the membrane of Bacillus megaterium. J. Mol. Biol. 110: 603–618.CrossRefGoogle Scholar
  202. 201.
    Rothman, J. E., and J. Lenard. 1977. Membrane asymmetry. Science 195: 743–753.PubMedCrossRefGoogle Scholar
  203. 202.
    Ruoho, A., and J. Kyte. 1974. Photoaffinity labeling of the ouabain-binding site on (Na+ + K+) adenosinetriphosphatase. Proc. Natl. Acad. Sci. U.S.A. 71: 2352–2356.PubMedCrossRefGoogle Scholar
  204. 203.
    Santillan, G., and J. K. Blasie. 1975. A direct analysis of lamellar x-ray diffraction from lattice-disordered retinal receptor disk membrane multilayers at 8 A resolution. Biophys. J. 15: 109a.Google Scholar
  205. 204.
    Schnebli, M. P., A. E. Vattoi, and A. Abrams. 1970. Membrane adenosine triphosphatase from Strepto-coccus faecalis, molecular weight, subunit structure, and amino acid composition. J. Biol. Chem. 245 (5): 1122–1127.PubMedGoogle Scholar
  206. 205.
    Schneider, D. L., Y. Kagawa, and E. Racker. 1972. Chemical modification of the inner mitochondrial membrane. J. Biol. Chem. 247: 4074–4079.PubMedGoogle Scholar
  207. 206.
    Segrest, J. R., T. Gulick-Krzywicki, and C. Sardet. 1974. Association of the membrane-penetrating polypeptide segments of the human erythrocyte MN-glycoprotein with phospholipid bilayers I. Formation of freeze-etch intramembranous particles. Proc. Natl. Acad. Sci. U.S.A. 71 (8): 3294–3298.PubMedCrossRefGoogle Scholar
  208. 207.
    Segrest, J. P., R. L. Jackson, and V. T. Marchesi. 1972. Red cell membrane glycoprotein: Amino acid sequence of an intramembranous region. Biochem. Biophys. Res. Commun. 49: 964–969.PubMedCrossRefGoogle Scholar
  209. 208.
    Shafer, P. T. 1974. The interaction of polyamino acids with lipid monolayers. Biochim. Biophys. Acta 373: 425–435.PubMedCrossRefGoogle Scholar
  210. 209.
    Shah, D. O. 1969. Lipid-protein interaction in mono-layers. Effects of conformation on poly-L-lysine on stearic acid monolayers. Biochim. Biophys. Acta 193: 217–220.PubMedCrossRefGoogle Scholar
  211. 210.
    Sheridan, J. D. 1973. Functional evaluation of low resistance junctions; Influence of cell shape and size. Am. Zool. 13:1119–1128; McNutt, N. S., and R. S. Weinstein. 1973. Membrane ultrastructure at mammalian intercellular junctions. In: Progress in Biophysics and Molecular Biology, Vol. 26. J. Butler and D. Nobles, eds. Pergamon, Oxford. pp. 45–107.Google Scholar
  212. 211.
    Sherwood, D., and M. Montai 1975. Transmembrane lipid migration in planar asymmetric bilayer membranes. Biochem. J. 15: 417–434.Google Scholar
  213. 212.
    Shidlovsky, G. 1965. Contrast in multilayer systems. Lab. Invest. 14: 475–495.Google Scholar
  214. 213.
    Shimshick, E. J., W. Kleemann, W. L. Hubbell, and H. M. McConnell. 1973. Lateral phase separations in membranes. J. Supramol. Struct. 1 (4–5): 285.PubMedCrossRefGoogle Scholar
  215. 214.
    Simionescu, M., N. Simionescu, and G. E. Palade. 1975. Segmental differentiations of cell junctions in the vascular endothelium. J. Cell Biol. 67: 863–885.PubMedCrossRefGoogle Scholar
  216. 215.
    Singer, S. J. 1974. The molecular organization of membranes. Annu. Rev. Biochem. 43: 805–833.PubMedCrossRefGoogle Scholar
  217. 216.
    Singer, S. J. 1974. In: Electron Microscopy, Vol. II: Biologica, VIII Int. Cong. EM. J. V. Sanders and D. J. Goodchild, eds. Aust. Acad. Sci., Canberra. pp. 186–187.Google Scholar
  218. 217.
    Singer, S. J., and G. L. Nicholson. 1972. The fluid mosaic model of the structure of cell membranes. Science 175: 720–731.PubMedCrossRefGoogle Scholar
  219. 218.
    Sjöstrand, J. 1965. A new ultrastructure element of the membrane in mitochondria and some cytoplasmic membranes. J. Ultrastruct. Res. 9: 340–361.CrossRefGoogle Scholar
  220. 219.
    Sjöstrand, F. S. 1969. Morphological aspects of lipoprotein structures. In: Structural and Functional Aspects of Lipoprotein in Living Systems. E. Tria and A. M. Scanu, eds. Academic Press, New York. pp. 73139.Google Scholar
  221. 220.
    Sjöstrand, F. S., and E. Anderssen. 1954. Electron microscopy of the interrelated discs of cardiac muscle tissue. Experientia 10: 369–370.PubMedCrossRefGoogle Scholar
  222. 221.
    Sjöstrand, F. S., E. Andersson-Cedergren, and M. M. Dewey. 1958. The ultrastructure of the intercalacted discs of frog, mouse and guinea pig cardiac muscle. J. Ultrastruct. Res. 1: 271–287.PubMedCrossRefGoogle Scholar
  223. 222.
    Sommer, J. R., R. L. Steere, E. A. Johnson, and P. H. Jewitt. 1972. Ultrastructure of cardiac muscle. A comparative review with emphasis on the muscle fibers of the ventricles. Hibernation Hypothermia IV Symposium, Snowmass-at-Aspen, Colorado. F. E.South, J. P. Hanlon, J. R. Willis, E. T. Pengelley, and N. R. Alpert, eds. American Elsevier, New York.Google Scholar
  224. 223.
    Smith, D. S. 1963. The structure of flight muscle sarcosomes in the blowfly, Calliphora erythrocephala (Diptara). J. Cell Biol. 19: 115–138.PubMedCrossRefGoogle Scholar
  225. 224.
    Standish, M. M., and B. A. Pethica. 1968. Surface pressure and surface potential study of a synthetic phospholipid at the air/water interface. Trans. Faraday Soc. 64: 1113.CrossRefGoogle Scholar
  226. 225.
    Steck, T. L., G. Fairbanks, and D. F. H. Wallach. 1971. Disposition of the major proteins in the isolated erythrocyte membrane. Proteolytic dissection. Biochemistry 10: 2617–2624.PubMedCrossRefGoogle Scholar
  227. 226.
    Steere, R. L. 1957. Electron microscopy of structural detail in frozen biological specimens. J. Biophys. and Biochem. Cytol. 3: 45–60.CrossRefGoogle Scholar
  228. 227.
    Steere, R. L., and M. Moseley. 1969. New dimensions in freeze-etching. Proceedings of the Electron Microscopy Society of America, 27th Annual Meet-ing. C. J. Arceneaux, ed. Claitor’s, Baton Rouge.Google Scholar
  229. 228.
    Steim, J. M., N. E. Tourtellotte, J. C. McElhaney, and R. L. Rader. 1969. Calorimetric evidence for the liquid cyrstalline state of lipids in a biomembrane: Proc. Natl. Acad. Sci. U.S.A. 63: 104–109.PubMedCrossRefGoogle Scholar
  230. 229.
    Stoeckenius, W. 1962. Some electron microscopical observations on liquid-crystalline phases in lipid-water systems. J. Cell Biol. 12: 221–229.PubMedCrossRefGoogle Scholar
  231. 230.
    Stoeckenius, W. 1963. Some observations on negatively stained mitochondria. J. Cell Biol. 17: 443–454.PubMedCrossRefGoogle Scholar
  232. 231.
    Stoeckenius, W., and D. M. Engelman 1969. Current models for the structure of biological membranes. J. Cell Biol. 42: 613–646.PubMedCrossRefGoogle Scholar
  233. 232.
    Stoeckenius, W., and W. H. Kunau. 1968. Further characterization of particulate fractions from lysed cell envelopes of Halobacterium halobium. J. Cell Biol. 38: 337–357.CrossRefGoogle Scholar
  234. 233.
    Stoeckenius, W., and D. Oesterhelt. 1973. Functions of a new photoreceptor membrane. Proc. Natl. Acad. Sci. U.S.A. 70: 2853–2857.PubMedCrossRefGoogle Scholar
  235. 234.
    Stoeckenius, W., and R. Rowen. 1967. A morphological study of Halobacterium halobium and its lysis in media of low salt concentration. J. Cell Biol. 34: 365393.Google Scholar
  236. 235.
    Strittmatter, P., M. J. Roger, and L. Spatz. 1972. The binding of cytochrome b 5 to liver microsomes. J. Biol. Chem. 247: 7188–7194.PubMedGoogle Scholar
  237. 236.
    Tenenbaum, D., and J. Folch-Pi. 1966. The preparation and characterization of water-soluble proteolipid protein from bovine brain white matter. Biochim. Biophys. Acta 115: 141–147.PubMedCrossRefGoogle Scholar
  238. 237.
    Therun, W., and E. Mehl. 1970. Determination of molecular weights of microgram quantities of protein constituents from biological membranes and other complex mixtures: gel electrophoreasis across linear gradients of acrylamide. Biochim. Biophys. Acta 160: 132–134.Google Scholar
  239. 238.
    Thompson, T. E. 1960. The properties of bimolecular phospholipid membranes. In: Cellular Membranes in Development. M. Locke, ed. Academic Press, New York. pp. 83–96.Google Scholar
  240. 239.
    Tomino, S., and R. Paigen. 1975. Egasyn, a protein complexed with microsomal B. glucuronidase. J. Biol. Chem. 250: 1146–1148.Google Scholar
  241. 240.
    Tosteson, M. P., and D. C. Tosteson. 1971. Oxygen-dependent conductance across thin lipid bilayer membranes containing microcylic compounds. Biophys. J. 11: 116a.Google Scholar
  242. 241.
    Tosteson, M. T., and D. C. Tosteson. 1977. Glycophorin spans the bilayer. Biophys. J. 17: 86a.Google Scholar
  243. 242.
    Tzagoloff, T. and P. Meagher. 1971. Assembly of the mitochondrial membrane system. V. Properties of a dispersed preparation of the oligomycin sensitive ATPase of yeast mitochondria. J. Biol. Chem. 246: 7328–7336.PubMedGoogle Scholar
  244. 243.
    Unwin, P. N. T., and R. Henderson 1975. Molecular structure determination by electron microscopy of unstained crystalline specimens. J. Mol. Biol. 94: 425440.Google Scholar
  245. 244.
    VanDeenen, L. L. M.. U. T. M. Houtsmuller, G. A. deHaas. and E. Mulder. 1962. J. Pharm. Plurnnacol. 14: 429.CrossRefGoogle Scholar
  246. 245.
    Vergara, J., F. Zambrano, J. D. Robertson, and H. Elrod. 1974. Isolation and characterization of luminal membranes from urinary bladder. J. Cell Bio!. 61: 8394.Google Scholar
  247. 246.
    Vergara, J., W. Longley, and J. D. Robertson. 1969. A hexagonal arrangement of subunits in membrane of mouse urinary bladder. J. Mol. Bio!. 46:593–5%.Google Scholar
  248. 247.
    Verkleij, A. J., R. F. A. Zwaal, B. Roelofsen, P. Comfurius, D. Kastellijn. and L. L. M. Van Deenen. 1973. The asymmetric distribution of phospholipids in the human red cell membrane: A combined study using phospholipases and freeze-etch electron microscopy. Biochim. Biophys. Acta 323: 178–193.Google Scholar
  249. 248.
    Waldbillig, R. C., T. J. McIntosh, and J. D. Robertson. 1976. Images of molecular flip-flop in asymmetric lipid bilayers. Biophys. J. 16: 194a.Google Scholar
  250. 249.
    Waldbillig, R. C., J. D. Robertson, and T. J. McIntosh. 1976. Images of divalent cations in unstainedsymmetric and asymmetric lipid bilayers. Biochim. Biophys. Acta 448: 1–14.PubMedCrossRefGoogle Scholar
  251. 250.
    Wallach, D. F. H.. and P. H. Zahler. 1966. Protein conformations in cellular membranes. Proc. Natl. Acad. Sci. U.S.A. 56 :1552–1559.Google Scholar
  252. 250a.
    Warren, R. C. and R. M. Hicks. 1970. Structure of the subunits in the thick luminal membrane of rat urinary bladder. Nature 227: 280–281.PubMedCrossRefGoogle Scholar
  253. 251.
    Weinstein, R. S., and S. Bullivant. 1967. The application of freeze-cleaving techniques to studies on red blood cell fine structure. Blood 29:780`789.Google Scholar
  254. 252.
    Whiteley, N. M., and H. C. Berg. 1974. Amidination of the outer and inner surfaces of the human erythrocyte membrane. J. Mo!. Bio!. 87: 541–561.CrossRefGoogle Scholar
  255. 253.
    Wolfgram, F. 1966. A new proteolipid fraction of the nervous system-I. J. Neurochem. 13: 461–470.PubMedCrossRefGoogle Scholar
  256. 254.
    Wolfgram, F., and K. Kotorii. 1968. The composition of the myelin proteins of the peripheral nervous system-Ii. J. Neurochem. 15 :1291–1295.Google Scholar
  257. 255.
    Wood, J. G., and R. M. C. Dawson. 1974. Some properties of a major structural glycoprotein of sciatic nerve. J. Neurochem. 22: 627–630.PubMedCrossRefGoogle Scholar
  258. 256.
    Wu, C.-W., and L. Stryer. 1972. Proximity relationship in rhodopsin. Proc. Nat!. Acad. Sci. U.S.A. 69: 1104–1108.CrossRefGoogle Scholar
  259. 257.
    Yahara, I., and G. M. Edelman. 1975. Electron microscopic analysis of the modulation of lymphocyte re -ceptor mobility. Exp Cell Res. 91: 125–142.PubMedCrossRefGoogle Scholar
  260. 258.
    Yamada, K. M., B. S. Spooner, and N. K. Wessels. 1971. Ultrastructure and function of growth cones and axon of cultured nerve cells. J. Cell Bio!. 49: 614–635.CrossRefGoogle Scholar
  261. 259.
    Yamamoto, S., and J. O. Lampen. 1976. Membrane penicillinase of Bacillus licheniformis 749/C: Sequence and possible repeated tetrapeptide structure of the phospholipopeptide region. Proc. Nat!. Acad. Sci. U.S.A. 73: 1457–1461.CrossRefGoogle Scholar
  262. 260.
    Zampighi, G., and J. D. Robertson. 1973. Fine structure of the synaptic discs separated from the goldfish medulla oblongada. J. Cell Biol. 56: 92–105.PubMedCrossRefGoogle Scholar
  263. 261.
    Zampighi, G., and J. D. Robertson. 1977. On macula communicans structure. Biophys. J. 17: 31A.Google Scholar
  264. 262.
    Zzar, K. 1974. Mitochondria! inner membrane particles seen in sections of in situ large amplitude smaller mitochondria in rhizondernal cells of cress (Lepidium Satiuum L.) Bioenergetics 6: 57–68.Google Scholar

Copyright information

© Springer Science+Business Media New York 1980

Authors and Affiliations

  • J. David Robertson
    • 1
  1. 1.Department of AnatomyDuke University Medical CenterDurhamUSA

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