Site Directed Mutagenesis as a Tool to Study Enzyme Catalysis

  • John R. Coggins
Part of the Progress in Mathematics book series (NSSA)

Abstract

Although the use of site-directed mutagenesis to engineer proteins, which was introduced in the preceding chapter, is less than 10 years old there have already been many publications describing the application of this technique to the study of enzymes. In this chapter I shall give some examples to illustrate how enzymologists are using site-diected mutagenesis to study enzyme catalysis and at the same time discuss the advantages and the limitations of the technique. Further examples may be found in the several excellent reviews that have appeared recently (Blow et al., 1986; Fersht et al., 1986a; Leatherbarrow & Fersht, 1986; 1987; Oxender & Fox, 1987; Shaw, 1987; Wells and Estell, 1988).

Keywords

Serine Protease Mutant Enzyme Amino Acid Side Chain Protein Engineer Preceding Chapter 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • John R. Coggins
    • 1
  1. 1.Department of BiochemistryUniversity of GlasgowGlasgowScotland

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