General Methods for Handling Proteins
In this chapter we shall review some general methods for the day-to-day handling of proteins, and some of the precautions that must be taken to ensure proper activity and stability of these biopolymers. Some useful methods for altering the solution conditions for protein samples will also be discussed here.
KeywordsAmmonium Sulfate Protein Solution Dialysis Tubing Molecular Weight Cutoff Handling Protein
Unable to display preview. Download preview PDF.
- Bollag, D. M., and Edelstein, S. J. (1991) Protein Methods, Wiley, New York.Google Scholar
- Cooper, T. (1977) The Tools of Biochemistry, Wiley, New York.Google Scholar
- Deutscher, M. P. (1990) Guide to Protein Purification (Meth. Enzymol., 182), Academic Press, San Diego.Google Scholar
- Fersht, A. (1985) Enzyme Structure and Function, 2d ed., W. H. Freeman, New York.Google Scholar
- Scopes, R. K. (1982) Protein Purification: Principles and Practice, Springer-Verlag, New York.Google Scholar
- Seed, B. (1989) in Short Protocols in Molecular Biology (Ausubel, F. M. et al., Ed.) Wiley, New York, p. 365.Google Scholar