Ethanol Induced Changes on in Vitro Protein Synthesis during the Development and Maturation of Brain Tissue
Decreased protein synthesis in a cell free system of brain has been reported for male adult rats following chronic ethanol ingestion. To assess the developmental and maturational changes occurring in the neonatal brain, the effects of pre- and postnatal maternal ingestion of ethanol were determined. For these studies young female rats were given a 10% w/v ethanol/water solution for varying periods after impregnation and seven days post pregnancy. Data showed that maternal ethanol ingestion produced a large deficit in the in vitro incorporation of (14C) leucine into the hot TCA extractable residue of ribosomes of neonatal brain. Maximum inhibition was obtained when ethanol was given postnatally. To determine the molecular sites of ethanol’s action, ribosomes and pH 5 enzymes from the adult and neonatal brain were examined. Data showed that the highest activity was obtained with control neonatal brain enzymes and ribosomal fractions.
KeywordsEthanol Ingestion Fetal Alcohol Syndrome Neonatal Brain Ethanol Administration Enzyme Fraction
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- Balazs, R. and Richter, D., 1973, “Biochemistry of the Developing Brain,” Vol 1, W. Himwich, ed., Dekker, New York.Google Scholar
- Davison, A. N., 1977, “Biochemical Correlates of Brain Structure and Function,” A. N. Davison, ed., Academic Press, New York.Google Scholar
- Jones, K. L. and Smith, D. W., 1973, Recognition of the fetal alcohol syndrome in early infancy, Lancet, 2: 99–1001.Google Scholar
- Lajtha, A., Latzkovits, L. afld Toth, 1976, Comparison of turnover rates of proteins of the brain, liver, and kidney in mouse in vivo following longterm labeling, Biochim. Biophys. Acta, 425: 511–520.Google Scholar
- Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J., 1951, Protein measurements with folin phenol reagent, J. Biol. Chem., 193: 265–275.Google Scholar
- Rawat, A., 1975, Ribosomal protein synthesis in the fetal and neonatal rat brain as influenced by maternal ethanol consumption, Res. Comm. Chem. Path. Pharmacol., 12: 723–732.Google Scholar
- Tewari, S. and Noble, E. P., 1973, Protein and RNA metabolism in brains of mice following chronic alcohol consumption, Ann. N.Y. Acad. Sci., 215: 333.Google Scholar
- Tewari, S. and Noble, E. P., 1975, Chronic ethanol ingestion by rodents: effects on brain RNA, in: “Alcohol and Abnormal Protein Biosynthesis,” M. A. Rothschild, M. Oratz and S. S. Schreiber, eds., Pergamon Press, New York.Google Scholar