Changes in Liver Inorganic Pyrophosphate Content during Ethanol Metabolism

  • G. A. Cook
  • M. Todd King
  • R. L. Veech

Abstract

The injection of ethanol (70 mmol/kg body weight) into male Wistar rats fed ad libitum caused an increase within 15 min in the liver inorganic pyrophosphate (PPi) content from 0.012 ± 0.001 μmol/g (wet weight) to 0.029 ± 0.001 μmol/g. The injection of acetate (20 mmol/kg body weight) increased the liver PPi to 0.157 ± 0.075 µmol/g in 15 min. The alcohol dehydrogenase inhibitor 4-methylpyrazole blocked the accumulation of acetate and the increase in PPi was prevented. Disulfiram, an inhibitor of aldehyde dehydrogenase, did not prevent changes in redox state, but the accumulation of acetate in the liver was decreased and the increase in liver PPi content was diminished (0.019 ± 0.003 μmol/g). These data suggest that the increase in PPi observed after injection of ethanol may be due to the activation of the acetate produced during ethanol and acetaldehyde oxidation.

Keywords

Aldehyde Dehydrogenase Ethanol Metabolism Inorganic Pyrophosphatase Inorganic Pyrophosphate Ethanol Ethanol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1980

Authors and Affiliations

  • G. A. Cook
    • 1
  • M. Todd King
    • 1
  • R. L. Veech
    • 1
  1. 1.Laboratory of MetabolismNational Institute on Alcohol Abuse and AlcoholismRockvilleUSA

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