Effects of Chronic Ethanol Consumption on the Respiratory Chain of Rat Liver Submitochondrial Particles

  • William S. Thayer
  • Emanuel Rubin

Abstract

Studies of hepatic submitochondrial particles, which provide an experimental system allowing direct measurements of electron transfer independent of substrate transport reactions, showed that chronic ethanol ingestion (36% calories, 40 days) lowered the specific respiratory rates associated with substrate oxidation. NADH oxidase activity was decreased about 40%, succinoxidase was decreased 25%, and oxidation in ascorbate mediated by phenazine methosulfate was decreased 20%. The content of dithionite-reducible cytochrome aa 3 was decreased 38%, while that of cytochrome b was decreased 8%, and that of cytochromes c + c l was decreased 14%. Steady state kinetic measurements indicated that the turnover number of cytochrome oxidase was unchanged, about 15 s−1 under uncoupled conditions with NADH as substrate. When electron flux to cytochrome c was maximal, cytochrome c was maintained in a more highly reduced state relative to cytochrome aa 3 in submitochondrial particles from the ethanol-treated rat compared to those from the control rat. This finding is consistent with the greater decrease in cytochrome aa 3 content relative to that of cytochrome c. The results indicate that the diminished content of cytochrome oxidase is one of the factors responsible for the lower respiration rates caused by chronic ethanol consumption.

Keywords

Cytochrome Oxidase Chronic Ethanol Turnover Number NADH Oxidase Phenazine Methosulfate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1980

Authors and Affiliations

  • William S. Thayer
    • 1
  • Emanuel Rubin
    • 1
  1. 1.Department of Pathology, Department of Biological ChemistryHahnemann Medical CollegePhiladelphiaUSA

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