Effect of Ethanol on Hemoperfusion and O2 Sufficiency in Livers in Vivo
Hemoperfusion and rate of 02 uptake in the livers in vivo following ethanol ingestion were measured by reflectance spectrophotometry. Pressurization on the liver in situ above the sinusoidal blood pressure caused complete blocking of blood flow, followed by spectral changes due to transition from hepatic normoxia to anoxia. Analyses of such spectra provided informations as to the rate of 02 consumption in situ and the redox level of cytochrome c (+cl) in the mitochondrial respiratory chain. The rate of 02 consumption thus calculated remained constant until the apparent 02-saturation of hemoglobin in situ decreased to less than 10% of the total of hemoglobin. In parallel with the decrease of the rate of 02 consumption, the apparent reduction level of cytochrome c (+cl) increased. It was shown in fed rats that the ethanol ingestion (1 g/kg) stimulated 02 uptake in the liver by 29% which initially caused a decrease in 02-saturation of hemoglobin, followed by an increase in blood supply to the liver. Thus, the ethanol consumption resulted in an increase in hepatic oxidative metabolism, possibly leading to hepatic hypoxia and liver damage.
KeywordsReflectance Spectrum Ethanol Ingestion Chronic Ethanol Consumption Redox Level Respiratory Cytochrome
Unable to display preview. Download preview PDF.
- Israel, Y., J. Bernstein, and L. Videla, 1975, Liver hypermetabolic state after chronic ethanol consumption, Fed. Proc., 34:2052.Google Scholar
- Kessler, M., J. Hoper, and B.A. Krumme, 1976, Monitoring of tissue perfusion and cellular function, Anesthesiology, 45: 184.Google Scholar
- Sato, N., M. Shichiri, T. Matsumura, T. Kamada, H. Abe, and B. Hagihara, 1978, Non-destructive measurement of tissue oxidative functions, in: “Frontiers in Biological Energetics,” Dutton, P.L. et al., eds., Academic Press, New York.Google Scholar
- Sato, N., T. Kamada, M. Shichiri, S. Kawano, H. Abe, and B. Hagihara, 1978, The levels of the mitochondrial and microsomal cytochromes in drinker’s livers, Clin. Chim. Acta, 87:347.Google Scholar
- Sato, N., M. Shichiri, N. Hayashi, T. Kamada, H. Abe, and B. Hagihara, 1979, Behavior of cytochrome oxidase in living liver tissue, in: “Cytochrome Oxidase,” T.E. King, ed., Elsevier, North-Holland, Amsterdam.Google Scholar
- Sato, N., T. Kamada, M. Shichiri, S. Kawano, H. Abe, and B. Hagihara, 1979, Measurement of hemoperfusion and oxygen sufficiency in gastric mucosa in vivo, Gastroenterol., 76:814.Google Scholar
- Svoboda, D., and R. Manning, 1964, Chronic alcoholism with fatty metamorphosis of the liver mitochondrial alterations in hepatic cells, Amer. J. Path., 44:645.Google Scholar
- Thurman, R.G., and R. Scholz, 1977, Mechanism of increased oxygen uptake due to ethanol, in: “Alcohol and Aldehyde Metabolizing Systems,” R.G. Thurman et al., eds., Vol. III, pp. 99.Google Scholar