Sedimentation Equilibrium of Proteins in Density Gradients

  • James B. Ifft
Part of the Biological Separations book series (BIOSEP)

Abstract

The technique of equilibrium density-gradient ultracentrifugation in the analytical ultracentrifuge was first described by Meselson, Stahl, and Vinograd in 1957 (Meselson et al., 1957). The method provides a thermodynamically rigorous procedure for the determination of the buoyant density, hydration, and solvated molecular weight of polymers having molecular weights larger than about 10,000.

Keywords

Density Gradient Band Center Buoyant Density Analytical Ultracentrifuge Sedimentation Equilibrium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1975

Authors and Affiliations

  • James B. Ifft
    • 1
  1. 1.Department of ChemistryUniversity of RedlandsRedlandsUSA

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