Analysis of Hras1-Associated Polymorphisms and Segregation of Taq1-Defined Alleles in Different Human Tumors
Recently, specific restriction fragment length polymorphisms (RFLPs) of a number of different genomic regions have been found to be associated with increased risk for various human diseases (Caskey, 1987). RFLPs are DNA markers defined by a different pattern of restriction fragments obtained in Southern blots with genomic DNA, using probes free of repetitive sequences. Unlike classical expressed markers, RPLPs can be found in genomic sequences irrespective of whether they encode a protein or not. The possibility to approach the problem of a genetic susceptibility to cancer has been made possible by the identification and characterization of oncogenes whose somatic activation has been associated to the development of tumors (Weinberg, 1985). In fact, in addition to somatic changes in oncogene structure and/or expression which occur only in the tumor cell, the possibility must also be considered that constitutional differences in oncogene structure and regulation could influence the individual likelihood of developing tumors. This possibility is now testable since RFLPs have been detected for several of human oncogenes (Pearson et al., 1987; Biunno et al., 1988; Radice et al., 1988).
KeywordsMelanoma Patient Site Polymorphism Familial Melanoma Human Gene Mapping Variable Tandem Repeat
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