Thrombosis pp 31-49 | Cite as

Platelet Adherence to the Vessel Wall and to Collagen-Coated Surfaces

  • Jean-Pierre Cazenave
  • Marian A. Packham
  • Raelene L. Kinlough-Rathbone
  • J. Fraser Mustard
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 102)

Abstract

A rotating probe system has been developed to quantitate the adherence of 51Cr-labeled platelets to collagen-coated glass rods or to the subendothelium of the rabbit aorta. The platelets were suspended in a medium containing physiological concentrations of calcium and magnesium, albumin, and apyrase to prevent aggregation. Chelation of divalent cations in the medium by addition of sodium citrate, EDTA, or EGTA markedly diminished platelet adherence. These observations indicate that the interpretation of platelet adherence studies performed in the presence of these agents is questionable. Increasing albumin concentrations in the medium decreased the number of adherent platelets, whereas increasing the hematocrit increased platelet adherence. Thus the factors influencing platelet adherence were studied in the presence of 4% albumin and 40% hematocrit.

When the platelet surface was modified by treatment with thrombin, plasmin, trypsin, chymotrypsin, or ADP, platelet adherence to the surfaces was decreased. Removal of surface sialic acid by neuraminidase had no effect on adherence. Exposing the endothelial surface of an aorta to thrombin resulted in a marked increase in platelet adherence to the thrombin-treated surface; this effect was prevented by adding heparin to the platelet suspension. Platelet adherence to collagen and to subendothelium was inhibited by several drugs that are used in man: dipyridamole, RA 433, methylprednisolone, and the antibiotics penicillin G and cephalothin. Under the experimental conditions used, ASA did not inhibit adherence to collagen, although indomethacin and a high concentration of sulfinpyrazone did. None of these three drugs inhibited release of granule contents from the adherent platelets. Therefore, it is unlikely that they would inhibit smooth muscle proliferation in response to the platelet growth factor released from platelets adherent to collagen in the subendothelium at a site of vessel wall injury.

Keywords

Human Platelet Platelet Adherence Platelet Surface Rabbit Aorta Platelet Suspension 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • Jean-Pierre Cazenave
    • 1
    • 2
  • Marian A. Packham
    • 1
    • 2
  • Raelene L. Kinlough-Rathbone
    • 1
    • 2
  • J. Fraser Mustard
    • 1
    • 2
  1. 1.Department of PathologyMcMaster UniversityHamiltonCanada
  2. 2.Department of BiochemistryUniversity of TorontoCanada

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