Lipoxin Syntheses by Arachidonate 12- and 5-Lipoxygenases Purified from Porcine Leukocytes
Recently, we were successful in the purification of 12-lipoxygenase1 and 5-lipoxygenase2 from porcine leukocytes by immunoaffinity chromatography using their monoclonal antibodies which were raised with the crude enzyme preparations as antigen3, 4. In this chapter the lipoxin biosynthesis will be discussed as catalytic properties of these purified lipoxygenases.
KeywordsArachidonic Acid Sodium Borohydride Crude Enzyme Preparation Epoxy Compound Immunoaffinity Chromatography
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- 4.S. Kaneko, N. Ueda, T. Topai, T. Maruyama, T. Yoshimoto, and S. Yamamoto, Arachidonate 5-lipoxygenase of porcine leukocytes studied by enzyme immunoassay using monoclonal antibodies, J. Biol. Chem. 262:in press (1987).Google Scholar
- 10.B.J. Fitzsimmons, J. Adams, J.F. Evans, Y. Leblanc, and J. Rokach, The lipoxins: Stereochemical identification and determination of their biosynthesis, J. Biot. Chem. 260: 13008 (1985).Google Scholar