Lipoxins pp 15-26 | Cite as

Lipoxin Syntheses by Arachidonate 12- and 5-Lipoxygenases Purified from Porcine Leukocytes

  • Shozo Yamamoto
  • Natsuo Ueda
  • Chieko Yokoyama
  • Brian J. Fitzsimmons
  • Joshua Rokach
  • John A. Oates
  • Alan R. Brash

Abstract

Recently, we were successful in the purification of 12-lipoxygenase1 and 5-lipoxygenase2 from porcine leukocytes by immunoaffinity chromatography using their monoclonal antibodies which were raised with the crude enzyme preparations as antigen3, 4. In this chapter the lipoxin biosynthesis will be discussed as catalytic properties of these purified lipoxygenases.

Keywords

Arachidonic Acid Sodium Borohydride Crude Enzyme Preparation Epoxy Compound Immunoaffinity Chromatography 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Shozo Yamamoto
    • 1
  • Natsuo Ueda
    • 1
  • Chieko Yokoyama
    • 1
  • Brian J. Fitzsimmons
    • 2
  • Joshua Rokach
    • 2
  • John A. Oates
    • 3
  • Alan R. Brash
    • 3
  1. 1.Department of BiochemistryTokushima University School of MedicineTokushima 770Japan
  2. 2.Merck Frosst Canada, Inc.Pointe Claire-DorvalCanada
  3. 3.Department of PharmacologyVanderbilt University School of MedicineNashvilleUSA

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