Kinins—II pp 143-145 | Cite as

Method for Measurement of Human Urinary Kininase Activity

  • G. Porcelli
  • M. Di Jorio
  • M. Ranieri
  • L. Ranalli
  • L. D’Acquarica
Part of the Advances in Experimental Medicine and Biology book series (AEMB)

Abstract

A method was developed to measure kininase activity in human urine. The method consists of dialysis of human centrifuged urine sample against phosphate buffer and partial fractionation of A-50 Sephadex column. The enzymatic property of urinary kininase, which destroys bradykinin when incubated, is estimated from its effect on a definite amount of bradykinin, using rat uterus.

Keywords

Urinary Sample Human Urine Enzymatic Property Phosphate Solution Tyrod Solution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Erdös, E.G. and Yang, H.Y.T. (1970) Kíninases - in: Hdb of Exp. Pharmacol., Vol. XXV, Ed. by Erdös, E.G., Springer Verlag, N.Y., p. 289.Google Scholar
  2. Greco, A., Porcelli G., Di Jorio, M., Ranieri M., D’Acquarica, L. and Ranalli L., Altered urinary Excretion of human kininase activity in hypertension; same issueGoogle Scholar
  3. Greco, A., Porcelli, G., Rebuzzi, A.G., Di Jorio, M., Ranieri, M. and Ranalli L., Altered urinary excretion of human kininase activity in acute myocardial infarction; same issueGoogle Scholar
  4. Levine, B.W., Talamo, R.C. and Kazemi, H. (1973), Action and metabo- lism of bradykinin in the dog lung, J.Appl.Physiol., 34, 821PubMedGoogle Scholar
  5. Porcelli, G., Marini-Bettòlo, G.B., Di Jorio, M., Ranieri, M., Ranallí, L., D’Acquarica, L., Preliminary purification and biochemical characterization of human urinary kininase; same issue.Google Scholar

Copyright information

© Springer Science+Business Media New York 1979

Authors and Affiliations

  • G. Porcelli
    • 1
  • M. Di Jorio
    • 1
  • M. Ranieri
    • 1
  • L. Ranalli
    • 1
  • L. D’Acquarica
    • 1
  1. 1.Istituto di Chimica, Facoltà di MedicinaUniversità Cattolica S. Cuore, Roma-Centro Chimica Recettori del C.N.R.RomaItaly

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