Oxygen-Linked Electron Transfer and Energy Conversion in Rhodospirillum Rubrum

  • Javier Varela
  • Juan M. Ramírez
Part of the FEMS Symposium book series (FEMSS)

Abstract

Pigmented Rbodospirillum rubrum cells from dark chemotrophic cultures contain several pathways for the transfer of electrons from reduced substrates to O2. In order of decreasing H+ -translocating efficiency, they are: (i) a cytochrome (oxidase) pathway that is inhibited by low concentrations of KCN and by inhibitors of the cytochrome b.ci complex, but not by CO; (ii) a CO sensitive or alternative (oxidase) pathway that is partly blocked by inhibitors of the cytochrome b.c 1 complex; and (iii) a third pathway that operates in the presence of CO plus antimycin A and that is absent in the presence of CO plus myxothiazol and in a mutant which lacks rhodoquinone. In addition, a significant fraction of the O2 uptake activity remains when H+ translocation is completely blocked by inhibitors of electron transfer or mutations. Since the rate of respiratory electron transfer appears to be limited at the substrate level, it is difficult to make a direct estimation of the contribution of each pathway to the final rate of respiration. However, from the relative energy-transducing efficiency of the cytochrome and the CO sensitive pathways (as measured by the H+/O ratios in O2 pulses) and the final cell yields of C-limited cultures of wild type and cytochrome-oxidase deficient strains, it seems that the contribution of the cytochrome pathway to the energy-conserving O2 uptake of pigmented chemotrophic R. rubrum, growing on malic and glutamic acids as carbon sources, is close to 80%.

Keywords

Cytochrome Oxidase Photosynthetic Bacterium Terminal Oxidase Proton Translocation Cytochrome Oxidase Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    N. Pfennig, General physiology and ecology of photosynthetic bacteria, in: “The Photosynthetic Bacteria”, R. K. Clayton and W. R. Sistrom, eds., Plenum Press, New York (1978).Google Scholar
  2. 2.
    L. Smith and P. B. Binder, Oxygen-linked electron transport and energy conservation, in: “The Photosynthetic Bacteria”, R. K. Clayton and W. R. Sistrom, eds., Plenum Press, New York (1978).Google Scholar
  3. 3.
    T. Sasaki, Y. Motokawa and G. Kikuchi, Ocurrence of both a-type and b-type cytochromes as the functional terminal oxidases in Rhodopseudomonas sphaeroides, Biochim. Biophys. Acta 197: 284 (1977).Google Scholar
  4. 4.
    B. Wakim, B. Georg and J. Oelze, Regulation of respiration and cytochrome c oxidase activities in Rhodospirillum rubrum and Rhodaspirillum tenue during the reversible adaptation from phototrophic to chemotrophic conditions, Arch. Microbiol. 124: 97 (1980).Google Scholar
  5. 5.
    D. Zannoni and A. Baccarini-Melandri, Respiratory electron flow in facultative photosynthetic bacteria, in: “Diversity of bacterial respiratory systems”, J.C. Knowles, ed., CRC Press, Boca Raton (1980).Google Scholar
  6. 6.
    R. K. Poole, Bacterial cytochrome oxidases. A structurally and functionally diverse group of electron transfer proteins, Biochim. Biophys. Acta 726: 205 (1983).Google Scholar
  7. 7.
    J. Ramirez and L. Smith, Synthesis of ATP in intact cells of R. rubrum and R. spheroides on oxygenation or illumination, Biochim. Biophys. Acta 153: 466 (1968).CrossRefGoogle Scholar
  8. 8.
    H. H. Lampe and G. Drews, Differentiation of membranes from Rhodopseudomonas capsulata with respect to their photosynthetic and respiratory functions, Arch. Microbiol. 84: 1 (1972).Google Scholar
  9. 9.
    A. Thore, D.L. Keister and A. San Pietro, Studies on the respiratory system of aerobically (dark) and anaerobically (light) grown Rhodospirillum rubrum, Arch. Mikrobiol. 67: 378 (1969).CrossRefGoogle Scholar
  10. 10.
    C. Fenoll and J. M. Ramirez, Simultaneous presence of two terminal oxidases in the respiratory system of dark aerobically grown Rhodospirillum rubrum, Arch. Microbiol. 137: 42 (1984).Google Scholar
  11. 11.
    G. Venturoli, C. Fenoll and D. Zannoni, On the mechanism of respiratory and photosynthetic electron transfer in R. rubrum, Biochim. Biophys. Acta 892: 172 (1987).CrossRefGoogle Scholar
  12. 12.
    R. M. Wynn, F. G. Gaul, W. K. Choi, R. W. Shaw and D. B. Knaff, Isolation of cytochrome bci complexes from the photosynthetic bacteria Rhodopseudomonas viridis and Rhodospirillum rubrum, Photosynth. Res. Res. 9: 181 (1986).Google Scholar
  13. 13.
    C. Fenoll, S. Gómez-Amores, G. Giménez-Gallego and J. M. Ramirez, A single pool of cytochrome cz is shared by cytochrome oxidase and photoreaction centers in Rhodospirillum rubrum, in: “Advances in Photosynthesis Research”, C. Sybesma, ed., Martinus Nijhoff/Dr W. Junk Publishers, The Hague (1984).Google Scholar
  14. 14.
    M. P. Ramirez-Ponce, G. Giménez-Gallego and J. M. Ramirez, A specific role for rhodoquinone in the photosynthetic electron transfer system of Rhodospirillum rubrum, FEBS Lett. 114: 319 (1980).CrossRefGoogle Scholar
  15. 15.
    J. Imhoff, Quinones of photosynthetic purple bacteria, FEMS Microbiol. Lett. 25: 85 (1984).Google Scholar
  16. 16.
    G. Giménez-Gallego, S. del Valle-Tascón and J. M. Ramirez, A possible physiological function of the oxygen-photoreducing system of Rhodospirillum rubrum, Arch. Microbiol. 109: 119 (1976).Google Scholar
  17. 17.
    M. P. Ramirez-Ponce, J.M. Ramirez and G. Giménez-Gallego, Rhodoquinone as a constituent of the dark electron-transfer system of Rhodospirillum rubrum, FEBS Lett. 119: 137 (1980).CrossRefGoogle Scholar
  18. 18.
    J. Lascelles, The synthesis of porphyrins and bacteriochlorophylls by cell suspensions of Rhodopseudomonas sphaeroides, Biochem. J. 62: 78 (1956).PubMedGoogle Scholar
  19. 19.
    A. Verméglio and P. Richaud, Effect de l’antimycine A sur la respiration de cellules entières de Rhodopseudomonas sphaeroides, Physiol. Vég. 22: 581 (1984).Google Scholar
  20. 20.
    M. A. Taylor and J.B. Jackson, Proton translocation in intact cells of the photosynthetic bacterium Rhodopseudomonas capsulata, Biochim. Biophys. Acta 810: 209 (1985).CrossRefGoogle Scholar
  21. 21.
    H. W. van Verseveld and G. Bosnia, The respiratory chain and energy conservation in the mitochondrion-like bacterium Paracoccus denitrificans, Microbiol. Sci. 4: 329 (1987).Google Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Javier Varela
    • 1
  • Juan M. Ramírez
    • 1
  1. 1.Centro de Investigaciones BiológicasCSICMadridSpain

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