Recombination Sites within the I Region of the Mouse H-2 Complex

  • William P. Lafuse
  • Chella S. David
Part of the NATO ASI Series book series (NSSA, volume 144)

Abstract

Recombinant mouse strains have been important in the development of the genetic map of the H-2 complex and have been used to subdivide the H-2 complex into the K, D, Qa, Tla regions containing genes for class I MHC molecules; I region containing genes for class II MHC molecules; and S region containing genes for class III MHC molecules. A molecular map of the H-2 complex has also been developed with about 1,600 Kb of DNA having been cosmid cloned. The I region has been shown to consist of a 270 Kb segment of DNA that contains six class I genes: Aβ2, Aβ, Aα, Eβ, Eβ2 and Eα (Steinmetz et al. 1982). Over the last few years recombinant mouse strains with crossovers in the I region have been analyzed by DNA restriction fragment analysis and DNA sequencing (Steinmetz et al. 1982, 1986, Kobori et al. 1984, 1986, Lafuse et al. 1986, Lafuse and David, 1986, Begovich et al. 1985, Saha and Cullen 1986, Zimmerer et al. 1987). These studies have shown that recombination within the I region is not random but is limited to two specific locations. These sites which have been termed recombination hot spots are located within the Eβ and Eα genes. In this report we will review these studies and discuss the implication that there are haplotype specific sequences that promote recombination at these two sites. We will also report studies on additional recombinants mapping within the I region.

Keywords

Globin Gene Recombination Site Haplotype Combination Serological Typing Restriction Fragment Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Begovich, A.B., and Jones, P.P.: Free la Eα chain expression in the Eα +: Eβ recombinant strain A.TFR5. Immunogenetics 22: 523–532, 1985.PubMedCrossRefGoogle Scholar
  2. Kilpatrick, M.W., Klysik, J., Singleton, C.K., Zarling, D.A., Jovin, T.M., Harau, L.H., Erlanger, B.F., and Wells, R.D.: Intervening sequences of human fetal globin genes adopt left handed Z helices. J. Biol. Chem. 259: 7269–7277, 1984.Google Scholar
  3. Kobori, J.A., Winoto, A., McNicholas, J., and Hood, L.: Molecular characterization of the recombination region of six murine major histocompatibility complex (MHC) I-region recombinants. J. Mol. Cell. Immunol. 1: 125–131, 1984.PubMedGoogle Scholar
  4. Kobori, J.A., Strauss, E., Minard, K., and Hood, L.: Molecular analysis of the hot spot of recombination in the murine major histocompatibility complex. Science 234:173–179, 1986.PubMedCrossRefGoogle Scholar
  5. Lafuse, W.P., Berg, N., Savarirayan, S., and David, C.S.: Mapping of a second recombination hot spot within the I-E region of mouse H-2 gene complex. J. Exp. Med. 163: 1518–1528, 1986.PubMedCrossRefGoogle Scholar
  6. Lafuse, W.P., Savarirayan, S., McCormick, J.F., and David, C.S.: Identification of I-E alpha genes in H-2 recombinant mouse strains by F1 complementation. Transplantation 43: 297–302, 1987.PubMedCrossRefGoogle Scholar
  7. Lafuse, W.P., and David, C.S.: Recombination hot spots within the I region of the mouse H-2 complex map to the Eβ and Eα genes. Immunogenetics 24: 352–360, 1986.PubMedCrossRefGoogle Scholar
  8. Saha, B.K., and Cullen, S.E.: Molecular mapping of murine I region recombinants: Crossing over in the Eb gene. J. Immunol. 136: 1112–1116, 1986.PubMedGoogle Scholar
  9. Slightom, J.L. Bechl, A.E., and Smithies, O.: Human fetal Gy-Ay-globin genes: complete nucleotide sequences suggest that DNA can be exchanged between these duplicated genes. Cell 21: 627–633, 1980.PubMedCrossRefGoogle Scholar
  10. Steinmetz, M., Minard, S., Horvath, S., McNicholas, J., Frelinger, J., Wake, C., Long, E., Mach, B., and Hood, L.: A molecular map of the immune response region from the major histocompatibility complex of the mouse. Nature 300: 35–42, 1982.PubMedCrossRefGoogle Scholar
  11. Steinmetz, M., Stephan, D., and Lindahl, K.F.: Gene organization and recombination hot spots in the murine major histocompatibility complex. Cell 44: 895–904, 1986.PubMedCrossRefGoogle Scholar
  12. Zimmerer, E.J., Ogin, E., Shreffler, D.C., and H.C. Passmore: Molecular mapping of crossover sites within the I region of the mouse MHC. Analysis of ten recombinant chromosomes. Immunogenetics 25: 274–276, 1987.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • William P. Lafuse
    • 1
  • Chella S. David
    • 2
  1. 1.Department of Medical Microbiology and ImmunologyThe Ohio State UniversityColumbusUSA
  2. 2.Department of ImmunologyMayo Clinic and Medical SchoolRochesterUSA

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