H-2 Antigens pp 493-500 | Cite as

Visualization of Direct T-B Cell Interaction

  • Susan L. Swain
Part of the NATO ASI Series book series (NSSA, volume 144)

Abstract

The concept that an antigen-specific helper T cell directly binds to a B cell stimulated by the same antigen and that this direct interaction is essential for B cell antibody production is well accepted. The most convincing evidence until recently that such cell-cell contact was necessary came from studies of Mitchison (1971) and his colleagues, subsequently repeated in numerous permutations, which showed that the epitopes recognized by both the T cell and B cell must be present on the same molecule. The current picture we have of the events in this interaction is that the specific immunoglobulin on the B cell binds determinants on the antigen, that the antigen is internalized and processed into fragments, and that these fragments are somehow presented on the B cell surface in association with class II. A helper T cell then recognizes the antigen fragment-class II complex by virtue of its specific receptor and thus a direct interaction of the two cells occurs. The recognition of antigen/class II activates the T cells and stimulates the T cell to synthesize and secrete lymphokines. Both the direct interaction of the T cell with the B cell and the soluble lymphokines secreted by the helper T cell are considered essential for B cell activation and subsequent proliferation and differentiation to antibody secretion. Despite the wide acceptance of this scenario actual T-B cell interactions have not been directly visualized until recently, and the details of the interaction and its consequences are largely unknown. In this report, I will review several recent studies we have done in collaboration with our colleagues, Dr. Abraham Kupfer, Dr. Charles Janeway and Dr. Jonanthan Singer. These studies reveal several striking events which occur during specific T cell-B cell interaction.

Keywords

Golgi Apparatus Cell Couple Cell Antibody Production Cell Trigger Cell Effector Target 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Berke, C., 1983, Cytotoxic T lymphocytes. How do they function?, NJ ture. 162: 107.Google Scholar
  2. Bishop, G.A., and Haughton, G., 1986, Induced differentiation of a transformed clone of Lyl positive B cells by clonal T cells and antigen, Proc. Natl. Acad. Sci (USA) 83:7410.Google Scholar
  3. Cambier, J.C., Ransom, J.T., Harris, L.K., Coggeshall, K.M., Chen, Z.Z., Newell, and Justement, L.B., 1986, Coupling of B-cell surface Ig, la and BSF1 receptors to intracellar “second messengers.”, i.: “Proceedings of the Conference on Lymphocyte Activation and Immune Reaction,” S. Gupta, W.E. Paul, and A.S. Fauci ed., Plenum Press, New York, in press.Google Scholar
  4. Haskins, K., Hannum, C., White, J., Roehm, N., Kubo, R., Kappler, J., and Marrack, P., 1984, The major histocompatibility complex-restricted antigen receptor on T cells. VI. An antibody to a receptor allotype, J. Exp. Merl. 160:452.Google Scholar
  5. Gordon, M.Y., Riley, G.P., Watt, S.M., and Greaves, M.F., 1987, Compartmentalization of a haematopoietic growth factor (GM-CSF) by glycosaminoglycans in the bone marrow microenvironment, nature 326: 403.Google Scholar
  6. Kappler, J.W., White, J., Wegmann, R., Mustain, E., and Marrack, P., 1982, Antigen presentation by Ia+ B cell hybridomas, “‘roc. Natl. Acad. Sci (USA) 79:3604.Google Scholar
  7. Kaye, J., Procelli, S., Tite, J., Jones, B. & Janeway, C.A., 1983, Both a monoclonal antibody and antisera specific for determinants unique to individual cloned helper T cells can substitute for antigen and antigen-presenting cells in the activation of T cells. J. Exp. Med. 158:836.Google Scholar
  8. Kupfer, A., Louvard, D., and Singer, S.J., 1984, Polarization of the elements of the golgi apparatus with microtubules. Golgi apparatus and the microtubule-organizing center inGoogle Scholar
  9. cultured fibroblasts at the edge of an experimental wound, proc. Natl. Acad. Sci. (USA) 79:2603.Google Scholar
  10. Kupfer, A., Dennert, G., and Singer, S.J., 1985, The reorientation of the golgi apparatus and the microtubule organizing center in the cytotoxic effector cells is a prerequisite in the lysis of bound targets, J. Mol. Cell. Immunol. 2:37.Google Scholar
  11. Kupfer, A., Singer, S.J., and Dennert, G., 1986a, On the mechanism of unidirectional killing in mixtures of two cytotoxic T lymphocytyes. Unidirectional polarization of cytoplasmic organelles and the membane-associated cyto-Google Scholar
  12. skeleton in the effector cell, J. Exp. Med. 163:489.Google Scholar
  13. Kupfer, A., Swain, S., Janeway, C.A., and Singer, S., 1986b, The specific direct interaction of helper T cells and antigen-presenting B cells, Proc. Natl. Acad. Sri. (USA) 83:6080.Google Scholar
  14. Kupfer, A., Swain, S., and Singer, S.J., 1987a, The specific direct interaction of helper T cells and antigen-present-Google Scholar
  15. ing B cells. II. Reorientation of the microtubule organizing center and reorganization of the membrane associated cytoskeleton inside the bound helper cell, 1. Exp.Med. 165: 1565.Google Scholar
  16. Kupfer, A., Janeway, C.A., Singer, S.J., and Swain, S., 1987a, Co-clustering of CD4 (L3T4) with the T cell receptor is induced by specific direct interaction of helper T cellsGoogle Scholar
  17. and antigen-presenting cells, Proc. Natl. Acad. Sri. (USA), in press.Google Scholar
  18. Kupfer, A., and Singer, S.J., 1987, Membrane dynamics in cell-cell interactions, with application to immunology, in: The T Cell Receptor UCLA Symposium on Molecular and Cellular Biology, New Series, Vol. 73, eds, J. KapplerGoogle Scholar
  19. and M. Davis, Alan R. Liss, Inc., New York, in press.Google Scholar
  20. Kurt-Jones, E.A., Beller, D.I., Mizel, S.B., and Unanue, E.R., 1985, Identification of a membrane-associated interleukin 1 in macrophages, Proc. Natl Acad. Sci. (USA) 82:1204.Google Scholar
  21. Mitchison, N.A., 1971, The carrier effect in the secondary response to hapten-protein conjugates. II. Cellular cooperation, Eur. J. Tmmunol. 1:18.Google Scholar
  22. Noelle, R.J., Krammer, P.H., O’Hara, T., Uhr, J.W., and Vitetta, E.S., 1984, Increased expression of Ia antigens on resting B cells: An additional role for B cell growth factor, proc. Natl. Acad. Sri (USA) 81:6149.Google Scholar
  23. Podack, E.R., and Konigsberg, P.J., 1984, Cytolytic T cell granules. Isolation, structural, biochemical and functional characterization, J. Exp. Med. 160:695.Google Scholar
  24. Sanders, V.M. Synder, J.M., Uhr, J.W., and Vitetta, E.S., 1986, Characterization of the physical interaction between antigen-specific B and T cells. J. Tmmunol. 137:2395.Google Scholar
  25. Shimonkevitz, R., Kappler, J., Marrack, P., and Grey, H., 1983, Antigen recognition by H-2 restricted T cells. I.Google Scholar
  26. Cell-free antigen processing. J. Exp. Med. 158:303.Google Scholar
  27. Springer, T.A., Dustin, M.L., Kishimoto, T.K., and Martin, S.D., 1987, The lymphocyte function-associated LFA-1, CD-2 and LFA-3 molecules: Cell adhesion receptors of the immune system, Ann. Rev. Immunol. 5:223.Google Scholar
  28. Swain, S.L., 1983, T cell subsets and the recognition of MHC class, Immunol. RPV. 74:129.Google Scholar
  29. Swain, S.L., Dutton, R.W., Schwab, R., and Yamamoto, J., 1983 Xenogeneic human anti-mouse T cell responses are due to the same functional T cell subsets responsible for allospecific and major histocompatibility complex-restricted responses, J. Exp. Med 157: 720.Google Scholar
  30. Swain, S.L., and Dutton, R.W., 1987, Consequences of the direct interaction of helper T cell with B cells presenting antigen, Tmmunol. Rev. 99 in press.Google Scholar
  31. Swain, S.L., Kubota, E., and Dutton, R.W., 1987, Receptor redistribution and other signals in T and B cell triggering, in: The T Cell Receptor, UCLA Symposium on Molecular and Cellular Biology, New Series, Vol. 73, eds. J. Kappler and M. Davis, Alan R. Liss, Inc., New York, in press.Google Scholar
  32. Vitetta, E., Bessie, A., Fernandez-Botran, R., Myers, C.D., Oliver, K.G., Sanders, V.M., and Stevens, T.L., 1987, Interaction and activation of antigen-specific T and B cells. Tmmunol. Rev. 99, in press.Google Scholar

Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • Susan L. Swain
    • 1
  1. 1.Department of Biology and Cancer Center, Q-063University of CaliforniaSan Diego, La Jolla

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