Progesterone Receptors of Chick Oviduct
The chick oviduct progesterone receptor has been purified to homogeneity by affinity chromatography and its molecular action studied in vitro. The native receptor is a 200,000 MW dimer of two dissimilar 4S subunits with different intranuclear function. The receptors directly regulate RNA chain initiation sites in oviduct chromatin by interactions involving target tissue nuclear acceptor sites. There is a 1:1 correspondence between receptor “acceptor” sites and RNA sites. Only the dimer form of the receptor is active in vitro on chromatin templates. The study suggests a novel model for hormone action which can be tested directly in this system.
KeywordsProgesterone Receptor Initiation Site Ammonium Sulfate DEAE Cellulose Average Chain Length
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- Buller, R.E., Schwartz, R.J., Schrader, W.T., and O’Malley, B.W. (1976) Progesterone-binding components of chick oviduct: In vitro effect of receptor subunits on gene transcription. J. BioZ. Chem. 250: 801–808.Google Scholar
- Kuhn, R.W., Schrader, W.T., Smith, R.G., and O’Malley, B.W. (1975) Progesterone binding components of chick oviduct–X. Purification by affinity chromatography. J. BioZ. Chem. 250: 4220–4228.Google Scholar
- Liarakos, C.D., Rosen, J.M.,and O’Malley, B.W. (1973) Effect of estrogen on gene expression in the chick oviduct–II. Transcription of chick tritiated unique deoxyribonucleic acid as measured by hybridization in ribonucleic acid excess. Biochemistry 12: 2809–2816.Google Scholar
- Lill, H., Lill, U., Sippel, A., and Hartmann, G. (1970) In:Lepetit colloquium on RNA polymerase and transcription (Silvestri, L.G., ed.), p. 55, North Holland Publishing Co., Amsterdam.Google Scholar
- Lowry, 0.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265.Google Scholar
- Mainwaring, W.I.P., Wilce, P.A., and Smith, A.E. (1974) Studies on the form and synthesis of messenger ribonucleic acid in the rat ventral prostate gland, including its tissue-specific stimulation by androgens. Biochem. J. 137: 531–524.Google Scholar
- O’Malley, B.W., Schrader, W.T., and Spelsberg, T.C. (1973) In: Receptors for reproductive hormones (O’Malley, B.W. and Means, A.R., eds.), pp. 174–196, Plenum Publishing Corp., New York.Google Scholar
- O’Malley, B.W., Sherman, M.R., Toft, D.O., Spelsberg, T.C., Schrader, W.T., and Steggles, A.W. (1971) A specific oviduct target-tissue receptor for progesterone. Identification, characterization, partial purification, intercompartmental transfer kinetics and specific interaction with the genome. In: Adv. Biosci., Vol. 7 ( Raspé, G., ed.), pp. 213–234, Pergamon Press, New York.Google Scholar
- Palmiter, R.D. (1973) Rate of ovalbumin messenger ribonucleic acid synthesis in the oviduct of estrogen-primed chicks. J. Biol. Chem. 248: 6230–8260.Google Scholar
- Schwartz, R.J. Tsai, M.J., Tsai, S.Y., and O’Malley, B.W. (1975) Effect of estrogen on gene expression in the chick oviduct–V. Changes in the number of RNA polymerase binding and initiation sites in chromatin. J. Biol. Chem. 250: 5175–5182.Google Scholar
- Sherman, M.R., Corvol, P.L., and O’Malley, B.W. (1970) Progester- one-binding components of chick oviduct–I. Preliminary characterization of cytoplasmic components. J. BioZ. Chem. 245: 6085–6096.Google Scholar
- Sica, V., Parikh, I., Nola, E., Puca, G.A., and Cuatrecasas, P. (1973) Affinity chromatography and the purification of estrogen receptors. J. BioZ. Chem. 248: 6543–6558.Google Scholar