Destruction of Microsomal Calcium Pump Activity: A Possible Secondary Mechanism in BrCCl3 and CCl4 Liver Cell Injury

  • Richard O. Recknagel
  • Karen Lowrey
  • Robert L. Waller
  • Eric A. GlendeJr.
Part of the Advances in Experimental Medicine and Biology book series (AEMB)

Abstract

In vitro rat liver microsomes free of Fe2+ ions peroxidize minimally at 37° when NADPH and either CC14 or BrCC13 are added. Although the lipid peroxidation dependent on toxigenic haloalkanes in these Fe2+-free microsome systems is very low, it is considerably more efficient in causing loss of cytochrome P-450 and glucose6-phosphatase than is the far more vigorous lipid peroxidation dependent on presence of Fe2+ ions. In particular, the Ca2+-pump activity of isolated microsomes was almost completely destroyed when malonic dialdehyde (MDA) production was as little as 8 pg per gram equivalent microsomes. Moore et al. (1976) had shown previously that the capacity of liver microsomes to sequester Ca2+ was severely depressed 30 min after CC14 administration to rats. We have shown that this effect is already manifested within 3 min after CC14 administration, by which time peroxidative decomposition of microsomal lipids can be detected. The time course of the destruction of the liver microsomal Ca2+ pump after CC14 administration to rats was essentially identical to the time course of microsomal lipid peroxidation, as revealed by the appearance of conjugated diene configurations in microsomal lipids.

Keywords

Lipid Peroxidation Carbon Tetrachloride Malonic Dialdehyde Lipid Peroxidation Process Microsomal Lipid Peroxidation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1982

Authors and Affiliations

  • Richard O. Recknagel
    • 1
  • Karen Lowrey
    • 1
  • Robert L. Waller
    • 1
  • Eric A. GlendeJr.
  1. 1.Department of PhysiologySchool of MedicineCase Western Reserve UniversityClevelandUSA

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