Extracting and Characterizing Hydra RNA: Modifications to Allow Extraction of Undegraded Material in the Presence of High Levels of Degradative Enzymes
Because hydra is rich in degradative enzymes (i e., nucleases and proteases), the application of conventional techniques for the isolation of macromolecules invariably results in the isolation of degraded material (Voland, 1975). We describe herein a modified phenol—chloroform procedure for the extraction of total RNA from Hydra oligactis which overcomes these difficulties.
KeywordsTris Buffer Ribonucleic Acid Degradative Enzyme Potassium Acetate Degraded Material
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