Dissolving the Nematocyst Capsule Wall and Identifying its Protein Component(s)
Since the properties of the nematocyst capsule and thread undoubtedly play a significant role in the mechanism of nematocyst discharge, solubilization of these structures facilitates the chemical and physical analyses of some of their components. Analyses show that nematocysts contain a collagenlike protein (Lenhoff et al, 1957) linked by disulfide bonds (Blanquet and Lenhoff, 1966).
KeywordsSquash Preparation Electrophoretic Technique Procedure Qualitative Analysis Nematocyst Type Thiol Solution
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- Cleland, W. W. 1964. Dithiothreitol, a new protective reagent for SH groups, Biochemistry 3(4):480–482Google Scholar
- Fishman, L., and Levy, M. 1967. Studies on the nematocyst capsule protein from the sea anemone Metridium marginatum, Biol. Bull. Woods Hole Mass. 133:464–465Google Scholar
- Gordon, A. H. 1972. Electrophoresis of proteins in polyacrylamide and starch gels. Ir.: Laboratory Techniques in Biochemistry and Molecular Biology, vol. 1, part 1 ( T. S. Work and E. Work, eds.), North Holland, Amsterdam.Google Scholar
- Mariscal, R N. 1971. Effect of a disulfide reducing agent on the nematocyst capsules from some Coelenterates, with an illustrated key to nematocyst classification. in: Experimental Coelenterate Biology ( H. M. Lenhoff, L. Muscatine, and L. V. Davis, eds.), University of Hawaii Press, Honolulu, pp. 157–168.Google Scholar
- Yanagita, T. M. 1959. Physiological mechanism of nematocyst responses in sea anemone. 1. Effects of trypsin and thioglycolate upon isolated nematocysts..fpn. J. Zool. 12: 361–375.Google Scholar