Genome Structure of Mouse Hepatitis Virus: Comparative Analysis by Oligonucleotide Mapping

  • Michael M. C. Lai
  • Stephen A. Stohlman
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 142)


Several natural variants of mouse hepatitis viruses have been compared by the T1-oligonucleotide fingerprinting technique. In general, they have aiverged quite extensively. However, MHV-3, a hepatotropic strain, and A59, a nonpathogenic strain, were found to be extremely related. Yet, each of them contains 2–4 specific oligonucleotides. One of the MHV-3-specific oligonucleotides was mapped in 30S poly(A)-containing RNA or 6–7 Kb from the 3-end and the other near the 5′-end of the genome. These two genetic regions might be associated with viral pathogenicity. In addition, two JHM plaque variants, DL producing large plaques and DS producing small plaques, were also compared. They share almost all T1-oligonucleotides, but each contains one unique spot. The DL-specific oligonucleotide was mapped in 21S poly(A)-containing RNA or at about 4 kb from the 3′-end. Finally, the MHV genome was found to contain the “cap” structure, confirming that it is a positive-stranded RNA.


Infectious Bronchitis Virus Rous Sarcoma Virus Nonpathogenic Strain Mouse Hepatitis Virus Specific Spot 
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  1. Bailey, O.T., Pappenheimer, A.M., Cheever, F.S., and J.B. Daniels. 1949. A murine virus (JHM) causing disseminated encephalomyelitis with extensive destruction of myelin. II. Pathology. J. Exp. Med. 90: 195–212.CrossRefGoogle Scholar
  2. Calisher, C.H. and W.P. Rowe. 1966. Mouse hepatitis, Reo-3 and Theiler viruses. Nat. Cancer Inst. Monogr. 20: 67–75.PubMedGoogle Scholar
  3. Cheever, L.S., Daniels, J.B., Pappenheimer, A.M., Bailey, O.T. 1949. A murine virus (JHM) causing disseminated encephalomyelitis with extensive destruction of myelin. I. Isolation and biological properties of the virus. J. Exp. Med. 90: 181–194.PubMedCrossRefGoogle Scholar
  4. De Wachter, R. and W. Fiers. 1972. Preparative two dimensional polyacrylamide gel electrophoresis of P-labeled RNA. Anal. Biochem. 49: 184–197.PubMedCrossRefGoogle Scholar
  5. Dick, G.W., Niven, J. and A. Gledhill. 1956. A virus related to that causing hepatitis in mice (MHV). Brit. J. Exp. Path. 38: 90–96.Google Scholar
  6. Furuich, Y. and K. Miura. 1975. A blocked structure at the 5′ terminus of mRNA from cytoplasmic polyhedrosis virus. Nature 253: 374–375.CrossRefGoogle Scholar
  7. Gledhill, A. and O.C. Andrews. 1951. A hepatitis virus of mice. Brit. J. Exp. Path. 32: 559–568.PubMedGoogle Scholar
  8. Gledhill, A.W. and J.S.F. Niven. 1955. Latent virus as exemplified by mouse hepatitis virus (MHV). Vet. Rev. Annot. 1: 82–90.Google Scholar
  9. Herndon, R.M., Griffin, D.E., McCormick, U., and Weiner, L.P. 1975. Mouse hepatitis virus-induced recurrent demyelination. Arch. Neurol. 32: 32–35.PubMedCrossRefGoogle Scholar
  10. Hierholzer, J.C., Broderson, J.R. and F. Murphy. 1979. New strain of mouse hepatitis virus as the cause of lethal enteritis in infant mice. Inf. Immun. 24: 508–522.Google Scholar
  11. Keith, J. and H. Fraenkel-Conrat. 1975. Identification of the 5′-end of Rous sarcoma virus RNA. Proc. Natl. Acad. Sci. USA 72: 3347–3358.PubMedCrossRefGoogle Scholar
  12. Lai, M.M.C. and Stohlman, S.A. 1978. The RNA of mouse hepatitis virus. J. Virol. 26: 236–242.PubMedGoogle Scholar
  13. Manaker, R.A., Piczak, C., Miller, A. and M. Stanton. 1961. A hepatitis virus complicating studies with mouse leukemia. J. Natl. Canc. Inst. 27: 29–51.Google Scholar
  14. McIntosh, K. 1973. Coronaviruses: A comparative review. Curr. Top. Microbiol. Immunol. 63: 85–129.Google Scholar
  15. Nelson, J.B. 1952. Acute hepatitis associated with mouse leukemia. I. Pathologic features and transmission of the disease. J. Exp. Med. 196: 293–300.CrossRefGoogle Scholar
  16. Robb, J.A. and C.W. Bond. 1979. Pathogenic murine coronaviruses. I. Characterization of the biological behavior in vitro and virus-specific intracellular RNA of strongly neurotropic JHMV and weakly neurotropic A59V viruses. Virology 94: 352–370.PubMedCrossRefGoogle Scholar
  17. Rowe, W., Hartley, J. and W. Capps. 1963. Mouse hepatitis virus infection as a highly contagious, prevalent enteric infection of mice. Proc. Soc. Exp. Biol. Med. 112: 161–165.PubMedGoogle Scholar
  18. Shatkin, A.J. 1974. Methylated messenger RNA synthesis in vitro by purified reovious. Proc. Natl. Acad. Sci. USA 71: 3204–3207.PubMedCrossRefGoogle Scholar
  19. Stern, D.F. and S.I.T. Kennedy. 1980. Coronavirus multiplication strategy. I. Identification and characterization of virus-specific RNA. J. Virol. 34: 665–674.PubMedGoogle Scholar
  20. Stohlman, S.A. and M.M.C. Lai. 1979. Phosphoproteins of murine coronaviruses. J. Virol. 32: 672–675.PubMedGoogle Scholar
  21. Virelizier, J.L., Dayan, A.D. and Allison, A.C. 1975. Neuro-pathological effects of persistent infection of mice by mouse hepatitis virus (MHV-3). Infection and Immunity 12: 1127–1140.PubMedGoogle Scholar
  22. Wang, L.H., P.H. Duesberg, T. Robins, et al. 1977. The terminal oligonucleotides of avian tumor virus RNAs are genetically linked. Virology 82: 472–492.PubMedCrossRefGoogle Scholar
  23. Wege, H., A. Muller, and V. ter Meulen. 1978. Genomic RNA of the murine coronavirus of JHM. J. Gen. Virol. 41: 217–228.PubMedCrossRefGoogle Scholar
  24. Weiner, L.P.: Pathogenesis of demyelination induced by a mouse hepatitis virus (JHM virus). Arch. Neurol. 28: 298–303.Google Scholar

Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • Michael M. C. Lai
    • 1
  • Stephen A. Stohlman
    • 1
  1. 1.University of Southern California School of MedicineLos AngelesUSA

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