Antigenic Relationships of Coronaviruses Detectable by Plaque Neutralization, Competitive Enzyme Linked Immunoabsorbent Assay, and Immunoprecipitation

  • J. C. Gerdes
  • L. D. Jankovsky
  • B. L. DeVald
  • I. Klein
  • J. S. Burks
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 142)


The antigenic relationships of mouse coronaviruses JHM and A59, human viruses OC43 and 229E, and multiple sclerosis (MS) isolates SD and SK have been investigated by plaque neutralization, competitive enzyme linked immunoabsorbent assay, and immunoprecipitat ion.

A59, SK, or SD plaques are neutralized by antiserum prepared against homologous as well as heterologous virus. Plaque neutralization also demonstrated weak reactivity between SD or SK and mouse virus JHM but no reactivity with human coronavirus 229E. An antiserum prepared against human virus 0C43 neutralized viruses SD and SK but not mouse viruses A59 or JHM.

In a competitive enzyme linked immunoabsorbent assay (cELISA) the binding of antiserum prepared against MS isolate SK to bound SK antigen was inhibited to a comparable degree using 0C43, SD, or A59 viral antigens. Coronavirus 229E or uninfected cell antigens did not block the binding of anti-SK serum to bound antigen. However, a cELISA utilizing 0C43 as bound antigen and competing an anti-0C43 serum suggests that virus 0C43 may be more closely related to SK than A59.

Specific viral polypeptides that share antigenic determinants have been identified by immunoprecipitat ion of S35 methionine labeled viral infected cell extracts. Polypeptides of similar molecular weight were precipitated from A59, SD, or SK infected cell extracts by SD, SK, 0C43, or A59 antisera. Our data suggests that the mouse coronavirus A59, human coronavirus 0C43, and MS isolates SD and SK contain antigenically related polypeptides of similar molecular weight.


Neutral Ization Mouse Hepatitis Virus Human Coronavirus Antigenic Relationship Human Coronaviruses 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Bonner, WM and Laskey, RA: A film detection method for tritiumlabeled proteins and nucleic acids in polyacrylamide gels. Eur. J. Biochem. 46: 83–88, 1974.PubMedCrossRefGoogle Scholar
  2. 2.
    Bradburne, AF: Antigenic relationships amongst coronaviruses. Arch. ges Virusforsch. 31: 352–364, 1970.PubMedCrossRefGoogle Scholar
  3. 3.
    Burks, JS, DeVald, BL, Jankovsky, LD, and Gerdes, JC: Two coronaviruses isolated from central nervous system tissue of two multiple sclerosis patients. Science 209: 933–934, 1980.PubMedCrossRefGoogle Scholar
  4. 4.
    Cheever, FS and Mueller, JH: Epidemic diarrheal disease of suckling mice. l. Manifestations, epidemiology, and attempts to transmit the disease. Exp. Med. 85: 405–416, 1947.CrossRefGoogle Scholar
  5. 5.
    Gerdes, JC, Klein, I., DeVald, B., and Burks, JS: Multiple sclerosis virus isolates SK and SD are serologically related to murine coronaviruses A59 and JHM and human coronavirus 0C43, but not to human coronavirus 229E. J. Virol. Submitted.Google Scholar
  6. 6.
    Heine, JW, Honess, RW, Cassai, E, and Roizman, B: The proteins specified by herpes simplex virus. XII. The virion polypeptides of type 1 strains. J. Virol. 14: 640–651, 1974.PubMedGoogle Scholar
  7. 7.
    Hierholzer, JC, Broderson, JR, and Murphy, FA: New strain of mouse hepatitis virus as the cause of lethal enteritis in infant mice. Infect. Immun. 24: 508–522, 1979.PubMedGoogle Scholar
  8. 8.
    Hirano, N, Fujiwara, K, Hino, S, and Matumoto, M: Replication and plaque formation of mouse hepatitis virus (MHV-2) in mouse cell line DBT culture. Arch. Gesamte Virusforsch 44: 298–302, 1974.PubMedCrossRefGoogle Scholar
  9. 9.
    Hierholzer, JC, Suggs, MT, and Hall, EC: Standardized viral hemagglutination and hemagglutination-inhibition tests. II. Description and statistical evaluation. Appl. Microbiol. 18: 824–833, 1969.PubMedGoogle Scholar
  10. 10.
    Kaye, HS, Hierholzer, JC, and Dowdle, WR: Purification and further characterization of an “IBV-like”. virus (coronavirus). Proc. Soc. Exp. Biol. Med. 135: 457–463, 1970.PubMedGoogle Scholar
  11. 11.
    Kaye, HS and Dowdle, WR: Some characteristics of hemagglutination of certain strains of “IBV-Iike” virus. J. Infect. Dis. 120: 576–581, 1969.PubMedCrossRefGoogle Scholar
  12. 12.
    Kessler, SW: Rapid isolation of antigens from cells with staphylococcal protein A-antibody absorbent: parameters of the interaction of antigen-antibody complexes with protein A. J. Immunol. 115: 1617–1624, 1975.PubMedGoogle Scholar
  13. 13.
    Laemmli, UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227: 680–685, 1970.CrossRefGoogle Scholar
  14. 14.
    Lai, MMC and Stohlman, SA: RNA of mouse hepatitis virus. J. Virol. 26: 236–242, 1978.PubMedGoogle Scholar
  15. 15.
    McIntosh, K, Becker, WB, and Chanock, RM: Growth in suckling mouse brain of “IBV-I ike” viruses from patients with upper respiratory tract disease. Proc. Natl. Acad. Sci. 58: 2268–2273, 1967.PubMedCrossRefGoogle Scholar
  16. 16.
    McIntosh, KA, Kapikian, AZ, Hardison, KA, Hartley, JW, and Chanock, RM: Antigenic relationships among the coronaviruses of man and between human and animal coronaviruses. J. Immunol. 102: 1109–1118, 1969.PubMedGoogle Scholar
  17. 17.
    Pedersen, NC, Ward, J., and Mengeling, WL: Antigenic relationship of the Feline Infectious Peritonitis Virus to coronaviruses of other species. Arch. Virol. 58: 45–53, 1978.PubMedCrossRefGoogle Scholar
  18. 18.
    Rowe, WP, Hartley, JW, and Capps, Wl: Mouse hepatitis virus infection as a highly contagious, prevalent, enteric infection of mice. Proc. Soc. Exp. Biol. Med. 112: 161–165, 1963.PubMedGoogle Scholar
  19. 19.
    Sturman, LS, Holmes, KV, and Behnke, J: Isolation of coronavirus envelope glycoproteins and interaction with the viral nucleocapsid. J. Virol. 33: 449–462, 1980.PubMedGoogle Scholar
  20. 20.
    Voller, A, Bidwell, D, and Bartlett, A: Microplate enzyme immunoassays for the immunodiagnosis of virus infections. in Manual of Clinical Immunology. N.R. Rose, H. Friedman, ed., ASM, pp. 506–512, 1976.Google Scholar
  21. 21.
    Wagner, RR, Prevec, L, Brown, F, Summers, DF, Sokol, F, and MacLeod, R: Classification of rhabdovirus proteins: a proposal. J. Virol. 10: 1128–1230, 1972.Google Scholar

Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • J. C. Gerdes
    • 1
  • L. D. Jankovsky
    • 1
  • B. L. DeVald
    • 1
  • I. Klein
    • 1
  • J. S. Burks
    • 1
  1. 1.Department of Microbiology and Immunology Rocky Mountain Multiple Sclerosis CenterUniversity of Colorado Health Sciences CenterDenverUSA

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