Studies of TGEV Spike Protein Gp195 Expressed in E. Coli and by a TGE-Vaccinia Virus Recombinant

  • Sylvia Hu
  • Joan Bruszewski
  • Ralph Smalling
  • Jeffrey K. Browne
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 185)


The gene coding for the surface spike protein gp195 of TGEV has been cloned and expressed in E. coli in the form of fusion proteins. These proteins were isolated and used to immunize laboratory animals. All animals developed antibodies cross-reacting with the TGEV virion but failed to neutralize the virus. The entire gp195 gene was also inserted into vaccinia to generate a TGEV-vaccinia recombinant virus (vTGE) that expressed TGEV gp195. Animals vaccinated with vTGE produced neutralizing antibodies against both TGEV and vaccinia. These results suggest the potential use of the recombinant vTGE as a vaccine against TGEV infection.


Vaccinia Virus Protein GP195 Immunoperoxidase Staining Vaccinia Virus Recombinant Mouse Hepatitis Virus 
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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Sylvia Hu
    • 1
  • Joan Bruszewski
    • 1
  • Ralph Smalling
    • 1
  • Jeffrey K. Browne
    • 1
  1. 1.AmgenThousand OaksUSA

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