Epoxidation of Arachidonic Acid and Prostacyclin in the Kidney

  • Patrick Y-K. Wong
  • John C. McGiff
  • Michal Schwartzman
  • F. F. Sun
Part of the NATO ASI Series book series (NSSA, volume 95)


The renal epoxygenase has been demonstrated to be an active pathway for the conversion of arachidonic acid (AA) to 11,12-epoxide, 19-hydroxy and 20-hydroxy-eicosatetraenoic acids. The epoxygenase activity was found to be located predominantly in the renal cortex. This renal epoxygenase pathway also transformed PGI2 to a new, previously unreported, metabolite. This metabolite was isolated and identified by radio gas chromatography and gas chromatography-mass spectrometry as 5-hydroxy-6-keto-PGF. Its structure was further confirmed by comparison of the mass-spectra to that of the synthetic standard. The formation of 5-hydroxy-6-keto-PGF in the kidney suggested epoxidation of prostacyclin via the renal epoxygenase as an alternative pathway of PGI2 metabolism.


Arachidonic Acid Rabbit Kidney Epoxyeicosatrienoic Acid Cytochrome P450 Enzyme System Dihydroxyeicosatrienoic Acid 
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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Patrick Y-K. Wong
    • 1
  • John C. McGiff
    • 1
  • Michal Schwartzman
    • 1
  • F. F. Sun
    • 2
  1. 1.New York Medical CollegeN.YUSA
  2. 2.The Upjohn CompanyKalamazooUSA

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