Expression of TGEV Structural Genes in Virus Vectors
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TGEV is a Coronavirus that causes gastroenteritis in pigs, resulting in a high mortality of neonates. The TGEV virion contains three major structural polypeptides; a surface glycoprotein (spike or peplomer protein) with a monomeric Mr 200000, a glycosylated integral membrane protein observed as a series of polypeptides of Mr 28000–31000 and a basic phosphorylated protein (the nucleoprotein) of Mr 47000 associated with the viral genomic RNA (1). The genes encoding TGEV nucleoprotein (NP), integral membrane protein (E1) and peplomer (E2) have been cloned and sequenced from an avirulent laboratory strain, Purdue (2, 3, 4, 5) and a virulent British field isolate, FS772/70 (6, 7).
KeywordsVaccinia Virus Integral Membrane Protein Transmissible Gastroenteritis Virus Infected Cell Lysate Virus Expression Vector
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