Mechanisms of Transport for the Uptake and Release of Biogenic Amines in Nerve Endings

  • Donald F. Bogdanski
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 69)


Reports on the amine transport mechanism located at the plasma membrane of nerve endings have been extensively reviewed for general characteristics 30, 31, 49, and for the effects of electrolytes 46. The nerve ending is uncommon, or unique in the sense that the product of secretion is recaptured almost immediately by the secretory organ. Transport, therefore, may be a bidirectional phenomenon in nerve endings. As a result of investigations, a synaptic sub-unit is envisaged in which electrolytes play an important role in the mobilization of amine as well as for transport in both directions.


ATPase Activity Synaptic Transmission Biogenic Amine Nerve Ending Outward Transport 
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  1. 1.
    Berti, F., and Shore, P.A., A kinetic analysis of drugs that inhibit the adrenergic neuronal membrane amine pump. Biochem. Pharmacol. 16 (1967) 2091–2094.CrossRefPubMedGoogle Scholar
  2. 2.
    Blackburn, K.S., French, P.C., and Merrills, R.J., 5-hydroxy- tryptamine uptake by rat brain vitro. Life Sci. 6 (1967) 1653CrossRefPubMedGoogle Scholar
  3. 3.
    Blaszkowski, T.P., and Bogdanski, D.F., Possible role of sodium and calcium ions in retention and physiological release of norepinephrine by adrenergic nerve endings. Biochem. Pharmacol., 20 (1971) 3281–3294.CrossRefPubMedGoogle Scholar
  4. 4.
    Blaszkowski, T.P., and Bogdanski, D.F., Evidence for sodium dependent outward transport of the 3H-norepinephrine mobilized by calcium at the adrenergic synapse. Inhibition of transport by desipramine. Life Sci. II, Part I (1972) 867–876.CrossRefGoogle Scholar
  5. 5.
    Bogdanski, D.F., and Blaszkowski, T.P., Role of extravesicular adenosine triphosphate and apparent vesicular energy conservation reactions in retention of norepinephrine by adrenergic nerve endings. Neuropharmacol. 14 (1975) 11–20.CrossRefGoogle Scholar
  6. 6.
    Bogdanski, D.F., Blaszkowski, T.P., and Tissari, A.H., Mechanisms of biogenic amine transport and storage IV. Relationship between K+ and the Na+ requirement for transport and storage of 5-hydroxytryptamine and norepinephrine in synaptosomes. Biochim. Biophys. Acta. 211 (1970) 521–532.CrossRefPubMedGoogle Scholar
  7. 7.
    Bogdanski, D.F., and Brodie, B.B., Role of sodium and potassium ions in storage of norepinephrine by sympathetic nerve endings. Life Sci. 5 (1966) 1563–1569.CrossRefPubMedGoogle Scholar
  8. 8.
    Bopdanski, D.F., and Brodie, B.B., The effects of inorganic ions on the storage and uptake of % norepinephrine by rat heart slices. J. Pharmacol. Exp. Ther. 165 (1969) 181–189.Google Scholar
  9. 9.
    Bogdanski, D.F., Tissari, A.H., and Brodie, B.B., Role of sodium, potassium, ouabain and reserpine in uptake, storage and metabolism of biogenic amines in synaptosomes. Life Sci. 7 (1968) 419–428.CrossRefPubMedGoogle Scholar
  10. 10.
    Bogdanski, D.F., Tissari, A.H., and Brodie, B.B., Mechanism of transport and storage of bipgenic amines. III. Effects of sodium and potassium on kinetics of 5-hydroxytryptamine and norepinephrine transport by rabbit synaptosomes. Biochim. Biophys. Acta 219 (1970) 189–199.CrossRefPubMedGoogle Scholar
  11. 11.
    Brown, G.L., The Croonian Lecture, 1964. The release and fate of the transmitter liberated by adrenergic nerves. Proc. Roy. Soc. B. 162 (1965) 1–19.ADSGoogle Scholar
  12. 12.
    Boullin, D.J. Quoted by Costa, E. Interaction of drugs with adrenergic neurons. Pharm. Rev., 18 (1966) 577–597.PubMedGoogle Scholar
  13. 13.
    Chang, P., Euler, U.S. von, and Lishajko, F., Effects of 2,4- dinitrophenol on release and uptake of noradrenaline in guinea pig heart. Acta Phys. Scand. 85 (1972) 501–505.CrossRefGoogle Scholar
  14. 14.
    Colburn, R.W., Goodwin, F.K., Murphy, D.L., Bunney, W.E.,Jr., and Davis, J.M., Quantitative studies of norepinephrine uptake by synaptosomes. Biochem. Pharmacol. 17 (1968) 957–964.CrossRefPubMedGoogle Scholar
  15. 15.
    Coyle, J.T., and Snyder, S.H., Catecholamine uptake by synaptosomes in homogenates of rat brain: Stereo-specificity in different areas. J. Pharmacol. Exp. Ther. 170 (1969) 221–231.PubMedGoogle Scholar
  16. 16.
    Crane, R.K., Na+dependent transport in the intestine and other animal tissues. Fed. Proc. 24 (1965) 1000–1006.PubMedGoogle Scholar
  17. 17.
    Douglas, W.W., and Rubin, R.P., The role of calcium in the secretory response of the adrenal medulla to acetylcholine. J.Physiol. Lond. 159 (1961) 40–57.CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Eddy, A.A., and Hogg, M.C., Further observations on the inhibitory effect of extracellular potassium ions on the uptake of glycine by mouse ascites tumour cells. Biochem. J. 114 (1969) 807–814.CrossRefPubMedPubMedCentralGoogle Scholar
  19. 19.
    Escueta, A.V., Appel, S.H., The effects of electroshock seizures on potassium transport within synaptosomes from rat brain. J. of Neurochem. 19 (1972) 1625–1638.CrossRefGoogle Scholar
  20. 20.
    Gage, P.W., and Quastel, D.M.J., Competition between sodium and calcium ions in transmitter release at mammalian neuromuscular junction. J. Physiol. Lond. 185 (1966) 95–123.CrossRefPubMedPubMedCentralGoogle Scholar
  21. 21.
    Garcia, A.G., and Kirpekar, S.M., Release of noradrenaline from the cat spleen by sodium deprivation. Br. J. Pharmacol. 47 (1973) 729–747.CrossRefPubMedPubMedCentralGoogle Scholar
  22. 22.
    Gillis, C.N., and Paton, D.M., Cation dependence of sympathetic transmitter retention by slices of rat ventricle. Br. J. Pharmacol. Chemether. 29 (1967) 309–318.CrossRefGoogle Scholar
  23. 23.
    Goldner, A.M., Schultz, S.G., and Curran, P.F., Sodium and sugar fluxes across the mucosal border of rabbit ileum. J. Gen. Physiol. 53 (1969) 362–383.CrossRefPubMedPubMedCentralGoogle Scholar
  24. 24.
    Green, R.D. III, and Miller, J.W., Evidence for the active transport of epinephrine and norepinephrine by the uterus of the rat. J. Pharmacol. Exp. Ther. 152 (1966) 42–50.PubMedGoogle Scholar
  25. 25.
    Harris, J.E., and Baldessarini, R.J., The uptake of 3H-dopainine by homogenates of rat corpus striatum: effects of cations. Life Sci. 13 (1973) 303–312.CrossRefPubMedGoogle Scholar
  26. 26.
    Heinz, E. (Ed.) Na-linked transport of organic solutes. Springer- Verlag, Berlin, 1972.Google Scholar
  27. 27.
    Hitzemann, B.A., Hitzemann, R.J., and Loh, H.H., On the specificity of trypsin (EC of nerve ending particles to inhibit norepinephrine transport. J. Neurochem. 24 (1975) 323–330.CrossRefPubMedGoogle Scholar
  28. 28.
    Holz, R.W., and Coyle, J.T., The effects of various salts, temperature and the alkaloids veratridine and batrachotoxin on the uptake of [3H]-dopamine into synaptosomes from rat striatum. Mol. Pharmacol. 10 (1974) 746–758.Google Scholar
  29. 29.
    Iversen, L.L., The uptake of noradrenaline by the isolated perfused rat heart. Br. J. Pharmacol. 21 (1963) 523–537.Google Scholar
  30. 30.
    Iversen, L.L., The uptake and storage of noradrenaline in sympathetic nerves. Cambridge University Press, New York, 1967.Google Scholar
  31. 31.
    Iversen, L.L., Neuronal uptake processes for amines and amino acids. In E. Costa and E. Giacobini (Eds.), Advances in Biochemical Psychopharmacology 2 (1970) 109–132.Google Scholar
  32. 32.
    Iversen, L.L., and Kravitz, E.A., Sodium dependence of transmitter uptake at adrenergic nerve terminals. Mol. Pharmacol. 2 (1966) 360–362.PubMedGoogle Scholar
  33. 33.
    Keen, P., and Bogdanski, D.F., Sodium and calcium ions in uptake and release of norepinephrine by nerve endings. Am. J. Physiol. 219 (1970) 677–682.CrossRefPubMedGoogle Scholar
  34. 34.
    Lee, C.O., and Armstrong, W. McD., Activities of sodium and potassium ions in epithelial cells of small intestine. Science 175 (1972) 1261–1264.ADSCrossRefPubMedGoogle Scholar
  35. 35.
    Ling, C.M., and Abdel-Latif, A.A., Studies on sodium transport in rat brain nerve ending particles. J. Neurochem. 15 (1968) 721–729.CrossRefPubMedGoogle Scholar
  36. 36.
    Ling, G.N., and Ochsenfeld, M.M., Mobility of potassium ion in frog muscle cells, both living and dead. Science 181 (1973) 78–81.ADSCrossRefPubMedGoogle Scholar
  37. 37.
    Maxwell, R.A., Keenan, P.D., Chaplin, E., Roth, B., Batmanglidje, S., Eckhardt, S., Molecular features affecting the potency of tricyclic antidepressants and structurally related compounds as inhibitors of the uptake of tritiated norepinephrine by rabbit, aortic strips. J. Pharmacol. Exp. Therap. 166 (1969) 320–329.Google Scholar
  38. 38.
    Paton, D.M., Cation and metabolic requirements for retention of metaraminol by rat uterine horns. Br. J. Pharmacol. Chemotherap. 33 (1968) 277–286.CrossRefGoogle Scholar
  39. 39.
    Pietryzk, C., and Heinz, E., The sequestration of Na+, K+ and Cl- in the cellular nucleus and its energetic consequences for the gradient hypotheses of amino acid transport in Ehrlich cells. Biochim. Biophys. Acta. 352 (1974) 397–411.Google Scholar
  40. 40.
    Riggs, T.R., Walker, L.M., Christensen, H.N., Potassium migration and amino acid transport. J. Biol. Chem. 233 (1958) 1479–1484.PubMedGoogle Scholar
  41. 41.
    Schuberth, J., and Sundwall, A., Effects of some drugs on the uptake of acetylcholine in cortex slices of mouse brain. J. Neurochem. 14 (1967) 807–812.CrossRefGoogle Scholar
  42. 42.
    Schultz, S.G., and Curran, P.F., Coupled transport of sodium and organic solutes. Physiol. Rev. 50 (1970) 637–718.CrossRefPubMedGoogle Scholar
  43. 43.
    Sugrue, M.F., and Shore, P.A., The mode of sodium dependency of the adrenergic neuron amine carrier. Evidence for a second, sodium dependent, optically specific and reserpine sensitive system. J. Pharmacol Exp. Therap. 170 (1969) 239–245.Google Scholar
  44. 44.
    Sugrue, M.F., and Shore, P.A., Further evidence for a sodium- dependent, optically specific and reserpine-sensitive amine carrier mechanism at the adrenergic neuron. J. Pharmacol. Exp. Therap. 177 (1971) 389–397.Google Scholar
  45. 45.
    Tissari, A.H., and Bogdanski, D.F., Biogenic amine transport. VI. Comparison of the effects of ouabain and K+ deficiency on the transport of 5-hydroxytryptamine and norepinephrine by synaptosomes. Pharmacology 5 (1971) 225–234.CrossRefPubMedGoogle Scholar
  46. 46.
    Tissari, A.H., and Bogdanski, D.F., Effects of inorganic electrolytes on the membrane transport and metabolism of serotonin and norepinephrine by synaptosomes. In O. Eränkö (Ed.), Progress in Brain Research, Elsevier Publishing Co., Amsterdam, 1971, (34) pp. 292–302.Google Scholar
  47. 47.
    Tissari, A.H., Schönhöffer, P.S., Bogdanski, D.F., and Brodie, B.B. Mechanism of biogenic amine transport.II. Relationship between sodium and the mechanism of ouabain blockade of the accumulation of serotonin and norepinephrine by synaptosomes. Mol. Pharm. 5 (1969) 593–604.Google Scholar
  48. 48.
    Tissari, A.H., and Suurhasko, R.U.A., Transport of 5-HT in synaptosomes of developing rat brain. Acta Toxicol. 29, Suppl. 4 (1971) 59.Google Scholar
  49. 49.
    Titus, E.G., and Dengler, H.J., The mechanism of uptake of norepinephrine. Pharmacol. Rev. 18, Part I (1966) 525–535.PubMedGoogle Scholar
  50. 50.
    Vidaver, G.A., Glycine transport by hemolyzed and restored pigeon red cells. Biochemistry 3 (1964) 795–799.CrossRefPubMedGoogle Scholar
  51. 51.
    Vidaver, G.A., Transport of glycine by pigeon red cells. Biochemistry 3 (1964) 662–667.CrossRefPubMedGoogle Scholar
  52. 52.
    White, T.D., and Keen, P., The role of internal and external Na+ and K+ on the uptake of [3H] noradrenaline by synaptosomes prepared from rat brain. Biochim. Biophys. Acta 196 (1968) 285–295.CrossRefGoogle Scholar
  53. 53.
    White, T.D., and Keen, P., Effect of inhibitors of (Na+ + K+)- dependent adenosine triphosphatase on the uptake of norepinephrine by synaptosomes. Mol. Pharmacol. 7 (1971) 40–45.PubMedGoogle Scholar
  54. 54.
    White, T.D., and Paton, D.M., Effects of external Na+ and K+ on the initial rates of noradrenaline uptake by synaptosomes prepared from rat brain. Biochim. Biophys. Acta 266 (1972) 116–127CrossRefPubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • Donald F. Bogdanski
    • 1
  1. 1.Section on Biochemical Pharmacology, Hypertension-Endocrine BranchNational Heart and Lung Institute, National Institutes of HealthBethesdaUSA

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