The Effect of Prostaglandins, Epinephrine and Aspirin on Cyclic AMP Phospho Diesterase Activity of Human Blood Platelets and Their Aggregation

  • M. S. Amer
  • N. R. Marquis
Conference paper
Part of the Alza Conference Series book series (ALZA, volume 1)


Kloeze (1967) reported that low concentrations of PGE(1) inhibited adenosine diphosphate (ADP)-induced platelet aggregation in plasma of pig, rat and man. Emmons et al (1967) observed that PGE(1) inhibited platelet aggregation induced by other physiological constituents: collagen, thrombin, norepinephrine and serotonin. The extreme potency of PGE(1) in preventing the aggregation phenomenon and its implication with the adenylate cyclic AMP system, as demonstrated by Butcher et al (1967), suggested that the prostaglandin effect might be mediated by cyclic AMP in platelets. Consequently, several laboratories at about the same time reported that PGE(1) increased the synthesis of cyclic AMP by platelet membrane fractions or lysates (Wolfe et al, 1969; Zieve et al, 1969; and Marquis et al, 1969) and by intact platelets (Salzman et al, 1969; Vigdahl et al, 1969).


Platelet Aggregation Adenylate Cyclase Platelet Rich Plasma Heavy Fraction Secondary Aggregation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Amer, M. S., 1971, Fed, Proc. 30:220.Google Scholar
  2. Amer, M. S., 1971a, J. Amer. Pharm. Assoc. NS11:118.Google Scholar
  3. Amer, M. S., 1972, In Proc. of the International Conf. on the Physiology and Pharmacology of Cyclic AMP. Ed. by R. Paoletti and G. A. Robison, Raven Press, New York.Google Scholar
  4. Amer, M. S., 1972a, J. Pharm. Exptl. Therap. (in press).Google Scholar
  5. Amer, M. S., and McKinney, G. R., 1970, The Pharmacologist 12:291.Google Scholar
  6. Ardlie, N. G., Glew, G., and Schwartz, C. J., 1967, Thromb. Diath. Haemorrh. 18:670.PubMedGoogle Scholar
  7. Atkinson, D. E., 1969, In Current Topics in Cellular Regulation. Ed. by B. L. Horecker and E. R. Stadtman, Academic Press, New York.Google Scholar
  8. Ball, G., Breneton, G. G., Fulwood, M., Ireland, D. M., and Yates, P., 1970, Biochem. J. 120:709.PubMedPubMedCentralGoogle Scholar
  9. Butcher, R. W., Scott, R. E., and Sutherland, E. W., 1967, The Pharmacologist 9:172.Google Scholar
  10. Butcher, R. W. and Sutherland, E. W., 1962, J. Biol. Chem. 237:1244.PubMedGoogle Scholar
  11. Cleland, W. W., 1970, In Enzymes. Ed. by P. D. Boyer, Academic Press, New York.Google Scholar
  12. Emmons, P. R., Hampton, J. R., Harrison, J. J. B., Honour, A. J., and Mitchell, J. R. A., 1967, Brit. Med. J. 2:468.CrossRefGoogle Scholar
  13. Guccione, M. A., Packham, M. A., Kinlaugh-Rathbone, R. L., and Mustard, J. F., 1971, Blood 37:542.CrossRefGoogle Scholar
  14. Jard, S. and Bernard, M., 1970, Biochem. Biophys. Res. Comm. 41:781–788.CrossRefGoogle Scholar
  15. Kakiuchi, S., Yamazaki, R., and Teshima, Y., 1971, Biochem. Biophys. Res. Comm. 42:968–974.CrossRefGoogle Scholar
  16. Kloeze, J., 1967, Proceed, of the 2nd Nobel Symposium, Stockholm. Ed. by S. Bergstrom and B. Samuelson, p. 241, Almquist and Wiksell, Stockholm.Google Scholar
  17. Kloeze, J., 1969, Biochem. Biophys. Acta. 187:285.CrossRefGoogle Scholar
  18. Marcus, A. J. and Zucker, M. B., 1965, The Physiology of Blood Platelets. Grune and Stratton, Inc., New York.Google Scholar
  19. Markwardt, F. and Hoffman, A., 1967, Biochem. Pharmac. 19:2510.Google Scholar
  20. Marquis, N. R., Becker, J. A., and Vigdahl, R. L., 1970, Biochem. Biophys. Res. Comm. 39:783.CrossRefGoogle Scholar
  21. Marquis, N. R., Vigdahl, R. L., and Tavormina, P. A., 1969, Biochem. Biophys. Res. Comm. 36:965.CrossRefGoogle Scholar
  22. Marquis, N. R., Vigdahl, R. L., and Tavormina, P. A., 1970, Atherosclerosis: Proc. of the 2nd International Symposium. Ed. by R. J. Jones, Springer-Verlag, New York.Google Scholar
  23. Mills, D. C. B., and Smith, J. B., 1971, Biochem. J. 121:185.CrossRefGoogle Scholar
  24. Moskowitz, J., Harwood, J. P., Reid, W. D., and Krishna, G., 1971, Biochem. Biophys. Acta. 230:279.CrossRefGoogle Scholar
  25. Mounter, L. A., and Turner, M. E., 1962, Enzymologia 25:225.Google Scholar
  26. Salzman, E. W., Kensler, P., and Levine, L., 1971, New York Academy of Sciences. Conference on Platelets and their Role in Hemostasis (in press).Google Scholar
  27. Salzman, E. W., and Levine, L., 1971, J. of Clin. Invest. 50:131.CrossRefGoogle Scholar
  28. Salzman, E. W., and Neri, L. L., 1969, Nature 224:610.CrossRefGoogle Scholar
  29. Salzman, E. W., Rubino, E. B., and Sims, R. V., 1970, Series Haematologica 3:100.PubMedGoogle Scholar
  30. Salzman, E. W., and Weisenberger, H., 1971, Proceed. of the International Conference on the Physiology and Pharmacology of Cyclic AMP. Raven Press, New York (in press).Google Scholar
  31. Smith, J. B., and Willis, A. L., 1971, Nature New Biology 231:235.CrossRefGoogle Scholar
  32. Sutherland, E. W., Robison, G. A., and Butcher, R. W., 1968, Circulation 37:279.CrossRefGoogle Scholar
  33. Thompson, W. J., and Appleman, M. M., 1971, J. Biol. Chem. 246:3145.PubMedGoogle Scholar
  34. Thompson, W. J., and Appleman, M. M., 1971a, Biochem. 10:311.CrossRefGoogle Scholar
  35. Vigdahl, R. L., Marquis, N. R., and Tavormina, P. A., 1969, Biochem. Biophys. Res. Comm. 37:409.CrossRefGoogle Scholar
  36. Vigdahl, R. L., Mongin Jr., J., and Marquis, N. R., 1971, Biochem. Biophys. Res. Comm. 42:1088.CrossRefGoogle Scholar
  37. Weeks, J. R., Chandra Sekhar, N., and Ducharme, D. W., 1968, J. Pharm. Pharmac. 21:103.CrossRefGoogle Scholar
  38. Wolfe, S. M., and Shulman, N. R., 1969, Biochem. Biophys. Res. Comm. 35:265.CrossRefGoogle Scholar
  39. Zieve, P. D., and Greenaugh, W. B., 1969, Biochem. Biophys. Res. Comm. 35:462.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1972

Authors and Affiliations

  • M. S. Amer
    • 1
  • N. R. Marquis
    • 1
  1. 1.Departments of Pharmacology and BiochemistryMead Johnson Research CenterEvansvilleUSA

Personalised recommendations