The culture of lymphoid and hematopoietic tissue in vitro is an important problem from both the theoretical and the practical points of view. Attempts to obtain cultures of these tissues in which their differentiation could continue have been undertaken repeatedly since 1910. It proved impossible to maintain hematopoiesis or lymphopoiesis for any length of time by explantation of hematopoietic and lymphoid tissues by classic experimental methods. Only recently, with the development of new tissue culture techniques and approaches, has it become possible to grow these tissues in vitro so that the constituent cells can continue to differentiate for a sufficient length of time. Although the problem of culturing hematopoietic and lymphoid tissue cannot be regarded as completely solved, the progress already made has provided the research worker with a valuable model with which to study lymphopoiesis and hematopoiesis and their regulation.
KeywordsOrgan Culture Lymphoid Tissue Nutrient Medium Cover Slip Plasma Clot
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