Selective Tropism of MHV3 for CNS Cells and Consequences of MHV3 Infection on CNS Cells Activities
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The presence of a receptor for a given virus on the cell membrane is a primary factor in determining the affinity of that virus for the cell and contributes to the pathogenicity of the disease (1). Studies of viral binding to isolated subpopulations of CNS cells provide an in vitro approach to the question of viral tropism in the nervous system and patterns of viral binding in vitro have been found to correlate with the pathologic consequences of viral infection in vivo. This has been well demonstrated in the reovirus system using recombinant viruses (2) as well as in other experimental systems including coronaviruses A59 or JHM-MHV4 (using temperature sensitive mutants) (3–6). The aim of our work was (a) to define the selective affinity of MHV3 for CNS cells and to compare it with that of other coronaviruses; (b) to analyse in vitro the consequences of MHV3 infection in CNS cells such as the ability of infected cells to survive and proliferate, the induction of cellular fusion, the intracyto-plasmic enzymatic activities and the membranal functions of the infected CNS cells.
KeywordsEpendymal Cell Infected Culture Viral Binding Infected Neuron Selective Affinity
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