Identification of the Coronavirus MHV-JHM mRNA 4 Gene Product Using Fusion Protein Antisera
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The murine hepatitis virus (MHV) genome is a large single-stranded RNA which encodes the major structural proteins of the virion, the nucleocapsid (N), the membrane (M) and the spike (S) proteins. Additionally, an unknown number of proteins which are either minor virion components, or non-structural proteins, are expressed during infection (Siddell, 1987). The expression of the MHV genome is mediated in the infected cell via a 3′ coterminal nested set of subgenomic mRNAs, which are numbered 1 to 7, in order of decreasing size. The available data (reviewed in Siddell, 1987) suggests that, at least for the structural protein mRNAs (i. e. mRNAs 3, 6 and 7) only the 5′ unique regions i. e. those absent from the next smallest mRNA, are translationally active and only single polypeptides are translated from these regions. In contrast, the unique region of mRNA 5 has two potential open reading frames (ORFs, Skinner et al., 1985) and although the translation products have not yet been identified, this unusual organization suggests that this mRNA may be functionally bicistronic.
KeywordsTranslation Product Unique Region Preimmune Serum Murine Hepatitis Virus Tamic Acid
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