Coronaviruses pp 343-349 | Cite as

Characterization of JHMV Variants Isolated from RAT Brain and Cultured Neural Cells after Wild Type JHMV Infection

  • F. Taguchi
  • S. Siddell
  • H. Wege
  • P. Massa
  • V. ter Meulen
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 218)


Mouse hepatitis virus (MHV) is a member of the coronavirus family, which are enveloped RNA viruses. Among MHV strains JHMV is of particular interest since this virus can produce a chronic demyelinating disease in rats (1, 2, 3) and mice (4, 5, 6). The intracerebral infection of 4 to 5-week-old Lewis rats with JHMV produces two distinct disease patterns. A subacute demyelinating ncephalitis which is seen in a minority of animals develops 12 to 14 days postinfection (p. i.)(2, 7). The transfer of lymphocytes from rats with subacute demyelinating encephalitis to uninfected recipient animals has recently been shown to induce an experimental allergic encephalitis-like disease (7). In contrast, most animals develop acute encephalitis, which leads to death within 14 days. During acute encephalitis, it was found that a virus with a larger mRNA3 as well as E2 glycoprotein compared with wild-type (wt) JHMV was selectively propagated in the brain of rats infected with wt JHMV. It was also observed that a. similar virus with a larger mRNA3 and E2 glycoprotein was selected in primary rat neural cell culture. These facts suggest that the virus with a larger E2 glycoprotein has a growth advantage in rat brain cells as compared with wt JHMV. In this paper, we describe these observations together with a detailed analysis of variants isolated from rat brain and cultured rat neural cells.


Neural Cell Mouse Hepatitis Virus Acute Encephalitis mRNA Pattern Chronic Demyelinating Disease 
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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • F. Taguchi
    • 1
  • S. Siddell
    • 1
  • H. Wege
    • 1
  • P. Massa
    • 1
  • V. ter Meulen
    • 1
  1. 1.Institute of VirologyUniversity of WurzburgWurzburgWest Germany

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