Coronaviruses pp 321-331 | Cite as

Increased Hepatotropism of Mutants of MHV, Strain JHM, Selected with Monoclonal Antibodies

  • Julian L. Leibowitz
  • James R. DeVries
  • Mosed Rodriguez
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 218)


The mouse hepatitis viruses (MHV) make up a group of closely related murine coronavi ruses which produce a wide spectrum of disease, depending upon the virus strain, the age and strain of the infected mouse, and the route of innoculation (Nelson, 1952; Gledhill and Neven, 1955). The determinants of the outcome of infection by MHV have been widely studied by many laboratories, with most groups concentrating their efforts on either the host response to the virus or on the viral genes responsible for virulence.


Infected Mouse Parental Virus Escape Mutant Mouse Hepatitis Virus Murine Coronavirus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Literature Cited

  1. Bailey, O.T. Pappenheimer, A.M., Cheever, F.S., and Daniels, J.B. 1949. A murine virus (JHM) causing disseminated encephalomyelitis with extensive destruction of myelin: II. Pathology. J. Exp. med. 90: 195–212.PubMedCrossRefGoogle Scholar
  2. Collins, A.R., Knobler, R.L., Powell, H., and Buchmeier, M.J. 1982. Monoclonal antibodies to murine hepatitis virus-4 (strain JHM) define the viral glycoproteins responsible for attachment and cell-cell fusion. Virology 119: 358–371.PubMedCrossRefGoogle Scholar
  3. Dalziel, R.G., Lampert, P.W., Talbot, P.J., and Buchmeier, M.J. 1986. Site-specific alteration of murine hepatitis virus type 4 peplomer glycoprotein E2 results in reduced neurovirulence. J. Virol. 59: 463–471.PubMedGoogle Scholar
  4. Fleming, J.O., Stohlman, S.A., Harmon, R.C., Lai, M.M.C., Frelinger, J.A., and Weiner, L.P. 1983. Antigenic relationships of murine coronavi ruses: analysis using monoclonal antibodies to JHM (MHV-4) virus. Virology 131: 296–307.PubMedCrossRefGoogle Scholar
  5. Fleming, J. O., Trousdale, M.D., El-Zaatari, F.A.K., Stohlman, S.A., and Weiner, L.P. 1986. Pathogenicity of antigenic variants of murine coronavirus JHM selected with monoclonal antibodies. J. Virol. 58: 869–875.PubMedGoogle Scholar
  6. Gledhill, A.W. and Neven, J.S.F. 1955. Latent virus as exemplified by mouse hepatitis virus. Vet. Rev. Annot. 1: 82–90.Google Scholar
  7. Knobler, R.L., Tunison, L.A., Lampert, P.W., and Oldstone, M.B.A. 1982. Selected mutants of mouse hepatitis virus type 4 (JHM strain) induce different CNS diseases. Am. J. Pathol. 109: 157–168.PubMedGoogle Scholar
  8. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685.PubMedCrossRefGoogle Scholar
  9. Lai, M.M.C., Brayton, P.R., Armen, R.C., Patton, C.D., Pugh, C., and Stohlman, S.A. 1981. Mouse hepatitis virus MHV A59: mRNA structure and genetic localization of the sequence divergence from hepatotropic strain MHV-3. J. Virol. 39: 823–834.PubMedGoogle Scholar
  10. Lampert, P.W., Sims, J.K., and Kniazeff, A.J. 1973. Mechanism of demyelination in JHM virus encephalomyelitis. Electron microscopic studies. Acta Neuropathol. (Berlin) 24: 76–85.CrossRefGoogle Scholar
  11. Leibowitz, J.L., Fung, L.S., and Levy, G.A. 1983. A sensitive radioimmunoassay for the determination of antibodies to mouse hepatitis virus. J. Virol. Meth. 6: 255–266.CrossRefGoogle Scholar
  12. Leibowitz, J.L., Weiss, S.R., Paavola, E., and Bond, C.W. 1982. Cell-free translation of murine coronavirus RNA. J. Virol. 43: 905–913.PubMedGoogle Scholar
  13. Nelson, J.B. 1952. Acute hepatitis associated with mouse leukemia. I. Pathologic features and transmission of the disease. J. Exp. Med. 96: 293–302.PubMedCrossRefGoogle Scholar
  14. Powell, H.C. and Lampert, P.W. 1975. Oligodendrocytes and their myelin-plasma membrane connections in JHM mouse hepatitis virus Encephalomyelitis. Lab. Invest. 33: 440–445.PubMedGoogle Scholar
  15. Robb, J.A. and Bond, C.W. 1979. Pathogenic murine coronavi ruses. I. Characterization of biological behavior in vitro and virus specific intracellular RNA of strongly neurotopic JHMV and weakly neurotropic A59V viruses. Virology 94: 352–370.PubMedCrossRefGoogle Scholar
  16. Rothfels, K.H., Axelrad, A.A., Siminovitch, L., McCulloch, E.A., and Parker, R.C. 1959. The origin of altered cell lines from mouse, monkey, and man as indicated by chromosome and transplantation studies. Can. Cancer Conf. 3: 189–214.Google Scholar
  17. Sturman, L.S. and Takemoto, K.K. 1972. Enhanced growth of a murine coronavirus in transformed mouse cells. Infect. Immun. 6: 501–507.PubMedGoogle Scholar
  18. Wege, H., Dorries, R., and Wege, H. 1984. Hybridoma antibodies to the murine coronavirus JHM: Characterization of epitopes on the peplorner protein (E2). J. Gen. Virol. 65: 1931–1942.PubMedCrossRefGoogle Scholar
  19. Weiner, L.P. 1973. Pathogenesis of demyelination induced by mouse hepatitis virus (JHM virus). Arch. Neurol. 28: 298–303.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Julian L. Leibowitz
    • 1
  • James R. DeVries
    • 1
  • Mosed Rodriguez
    • 2
  1. 1.Department of Pathology and Laboratory MedicineUniversity of Texas Health Science CenterHoustonUSA
  2. 2.Department of NeurologyMayo ClinicRochesterUSA

Personalised recommendations