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Coronaviruses pp 219-222 | Cite as

Expression of MHC Class I Genes in Mouse Hepatitis Virus (MHV-A59) Infection and in Multiple Sclerosis

  • Ehud Lavi
  • Akio Suzumura
  • Lois A. Lampson
  • Richard M. Siegel
  • Donna M. Murasko
  • Donald H. Silberberg
  • Susan R. Weiss
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 218)

Abstract

The alteration in expression of major histocompatibility complex (MHC) class I genes on neural cells in association with central nervous system (CNS) demyelination was studied. Infection of mice or glial cell cultures with a neurotropic demyelinating coronavirus, mouse hepatitis virus (MHV) strain A59, was used as a model system. While uninfected neural cells do not express detectable amounts of MHC class I surface antigens, after infection with MHV-A59 in vitro or in vivo, these antigens can be detected by reaction with a monoclonal antibody against H-2D and H-2K. Induction of MHC class I antigens by MHV-A59 infection in mice was associated with increased levels of H-2 mRNA. Upregulation of MHC class I antigens involved the production of soluble factor/s produced by infected astrocytes. The identity of the factor, which is not interferon, is at present unknown. Brain specimens from autopsies of patients with multiple sclerosis, a human inflammatory demyelinating diseases, were also examined for alteration of MHC antigens. Both class I and class II surface antigens were found in active plaques but not in chronic plaques or in normal brain. These results are consistent with the hypothesis that MHC class I antigen induction may play a role in inflammatory CNS demyelination in both experimental animals and in humans.

Keywords

Multiple Sclerosis Major Histocompatibility Complex Major Histocompatibility Complex Class Mouse Hepatitis Virus Major Histocompatibility Complex Antigen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Ehud Lavi
    • 1
    • 4
  • Akio Suzumura
    • 2
  • Lois A. Lampson
    • 3
  • Richard M. Siegel
    • 1
  • Donna M. Murasko
    • 1
    • 5
  • Donald H. Silberberg
    • 2
  • Susan R. Weiss
    • 1
  1. 1.Department of MicrobiologyUniversity of Pennsylvania School of MedicinePhiladelphiaUSA
  2. 2.Department of NeurologyUniversity of Pennsylvania School of MedicinePhiladelphiaUSA
  3. 3.Children’s Cancer Research Center of The Children’s Hospital of PhiladelphiaPhiladelphiaUSA
  4. 4.The Wistar Institute of Anatomy and BiologyPhiladelphiaUSA
  5. 5.Department of Microbiology and ImmunologyMedical College of PennsylvaniaPhiladelphiaUSA

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