Coronaviruses pp 169-174 | Cite as

Characterization of a Small Plaque Mutant of the A59 Strain of Mouse Hepatitis Virus Defective in Cell Fusion

  • Stanley G. Sawicki
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 218)


The virions of murine coronaviruses contain nucleocapsid protein and two membrane associated proteins E1 and E2. E2 or S glycoprotein forms the peplomer or spike protein and, as shown by Lawrence Sturman and Kathryn Holmes and co-workers (Sturman et al., 1985), is synthesized initially as a 180,000 Dalton glycoprotein that is cleaved into two 90,000 Dalton glycoproteins. They have also demonstrated that the cleaved form of E2 is required for cell fusion (Sturman et al., 1985) and that there are host-dependent differences in the proteolytic cleavage of E2 and in cell fusion (Frana et al., 1985). Recently, Stuart Siddell (Siddell, 1986) has sequenced the E2 gene of the JHM strain of mouse hepatitis virus (MHV) and found a protease cleavage site (arg-arg-ser) approximately in the middle of the open reading frame of RNA 3, the messenger RNA encoding E2.


Cell Fusion Proteolytic Cleavage Protease Cleavage Site Mouse Hepatitis Virus 17CL1 Cell 
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  1. Frana, M. F., Behnke, J. N., Sturman, L. S., and Holmes, K. V., 1985, Proteolytic cleavage of the E2 glycoprotein of murine coronavirus: host-dependent differences in proteolytic cleavage and cell fusion, J. Virol., 56: 912.PubMedGoogle Scholar
  2. Sawicki, S. G. and Sawicki, D. L., 1985, Coronavirus minus-strand RNA synthesis and effect of cycloheximide on coronavirus RNA synthesis, J. Virol., 57: 328.Google Scholar
  3. Siddell, S. G., 1986, In press.Google Scholar
  4. Sturman, L. S., Ricard, C. S., and Holmes, K. V., 1985, Proteolytic cleavage of the E2 glycoprotein of murine coronavirus: activation of cell-fusing activity of virions by trypsin and separation of two different 90K cleavage fragments, J. Virol., 56: 904.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Stanley G. Sawicki
    • 1
  1. 1.Department of MicrobiologyMedical College of OhioToledoUSA

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