Detection of MHV-A59 RNA by InSitu Hybridization
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We have shown that MHV-A59 causes a chronic demyelinating disease in C57BL/6 mice.1 Although active demyelination can be detected as long as five months post inoculation, it has been difficult to detect viral antigens by immunofluorescence and thus far impossible to demonstrate replication of infectious virus in chronically infected mice. Thus we have used in situ hybridization of CNS and liver samples with radiolabeled virus-specific DNA probes to investigate the state of the genome in these tissues. In this paper we report experiments in which we start to address the question of whether or not viral RNA can be detected in the CNS and liver of these chronically infected mice and, if so, how much and in which cell types.
KeywordsInfected Mouse Post Infection Viral Nucleic Acid Spinal Cord Section Mouse Hepatitis Virus
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