Cloning and Sequencing the Nucleocapsid and E1 Genes of Coronavirus

  • John Armstrong
  • Sjef Smeekens
  • Willy Spaan
  • Peter Rottier
  • Ben van der Zeijst
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 173)


The widespread medical and veterinary importance of Coronaviruses provided the initial reason for studying these viruses at the molecular level. However, two unusual features of the viral life cycle are of particular interest for “pure” molecular biology. First, the intracellular budding site of Coronaviruses, apparently associated with the restricted intracellular distribution of the E1 glycoprotein1 suggests that this protein may provide a model to study the transport and sorting of membrane proteins of the endoplasmic reticulum and Golgi apparatus. Secondly, the unusual “nested set” structure of the viral RNA’s2,3,4,5(Spaan et al., this volume) implies a replication mechanism unlike that of other RNA viruses.


Infectious Bronchitis Virus Viral Life Cycle Leader Region Mouse Hepatitis Virus Capsid Gene 
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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • John Armstrong
    • 1
  • Sjef Smeekens
    • 2
  • Willy Spaan
    • 2
  • Peter Rottier
    • 2
  • Ben van der Zeijst
    • 2
  1. 1.European Molecular Biology Laboratory69 HeidelbergGermany
  2. 2.Institute of Virology, Veterinary FacultyState University of UtrechtUtrechtThe Netherlands

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