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Plaque Assay, Polypeptide Composition and Immunochemistry of Feline Infectious Peritonitis Virus and Feline Enteric Coronavirus Isolates

  • John F. Boyle
  • Niels C. Pedersen
  • James F. Evermann
  • Alison J. McKeirnan
  • Richard L. Ott
  • John W. Black
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 173)

Abstract

The coronaviral nature of feline infectious peritonitis virus (FIPV) and feline enteric coronavirus (FECV) has been well documented by morphological, physicochemical and antigenic studies1–10. However, biochemical and detailed immunochemical analyses of FIPV and FECV have been difficult due to the inability to prepare sufficient quantities of viral material. Recently, we have been able to propagate FIPV and FECV in continuous cell culture of feline origin8, 11–13.

Keywords

Plaque Assay Infectious Bronchitis Virus Infected Cell Culture Feline Infectious Peritonitis Discontinuous Sucrose Gradient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • John F. Boyle
    • 1
  • Niels C. Pedersen
    • 1
  • James F. Evermann
    • 2
  • Alison J. McKeirnan
    • 2
  • Richard L. Ott
    • 2
  • John W. Black
    • 3
  1. 1.Department of Medicine, School of Veterinary MedicineUniversity of CaliforniaDavisUSA
  2. 2.Department of Veterinary Clinical Medicine and Surgery, School of Veterinary MedicineWashington State Univ.PullmanUSA
  3. 3.Specialized AssaysNashvilleUSA

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