Monoclonal Antibodies to the Three Classes of Mouse Hepatitis Virus Strain A59 Proteins
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Hybridoma cell lines producing monoclonal antibodies to mouse hepatitis virus strain A59 (MHV-A59) have been established by fusion of spleen cells of immunized mice with P3X63Ag8.653 mouse plasmacytoma cells. Culture fluids were screened for their ability to immunoprecipitate virus-specific proteins from 35S-methionine-labeled infected cells. Eleven clones were obtained which fell into three classes (Figure 1).