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Monoclonal Antibodies to the Three Classes of Mouse Hepatitis Virus Strain A59 Proteins

  • Marck J. M. Koolen
  • Albert D. M. E. Osterhaus
  • Kees H. J. Siebelink
  • Marian C. Horzinek
  • Bernard A. M. van der Zeijst
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Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 173)

Abstract

Hybridoma cell lines producing monoclonal antibodies to mouse hepatitis virus strain A59 (MHV-A59) have been established by fusion of spleen cells of immunized mice with P3X63Ag8.653 mouse plasmacytoma cells. Culture fluids were screened for their ability to immunoprecipitate virus-specific proteins from 35S-methionine-labeled infected cells. Eleven clones were obtained which fell into three classes (Figure 1).

Keywords

Infected Cell Spleen Cell Culture Fluid Immunize Mouse Nucleocapsid Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Reference

  1. 1.
    Siddell, S.G., J. gen. Virol. 62:259–269 (1982).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Marck J. M. Koolen
    • 1
  • Albert D. M. E. Osterhaus
    • 2
  • Kees H. J. Siebelink
    • 2
  • Marian C. Horzinek
    • 1
  • Bernard A. M. van der Zeijst
    • 1
  1. 1.Institute of Virology, Veterinary FacultyState University of UtrechtThe Netherlands
  2. 2.National Institute of Public HealthBilthovenThe Netherlands

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