Radiation Induced Reactions of Glutathione with Oxygen and their Possible Role in Biological Systems

  • M. Tamba
  • G. Simone
  • M. Quintiliani


The reactivity of some thiyl radicals with molecular oxygen were measured and reported much before the intracellular thiols began to arouse the wave of interest that has characterized the last decade or so (1,2). Cellular thiols, almost entirely consisting of glutathione (GSH), are considered at the present time as one of the most important system capable of protecting cells against free radicals formed during oxidative metabolism or from exposure to drugs or ionizing radiation.


Peroxyl Radical Pulse Radiolysis Thiyl Radical Opinion Reaction Italian National Research Council 
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  1. 1.
    J.P. Barton and J. E. Packer, The radiolysis of oxygenated cysteine solutions at neutral pH. The role of RSSR\(\overline \bullet \) and O\(\matrix{ {\overline \bullet } \cr 2 \cr } \). Int. J. Radiat. Phys. Chem. 2, 159–166 (1970).CrossRefGoogle Scholar
  2. 2.
    M. Quintiliani, R. Badiello, M. Tamba, A. Esfandi, and G. Gorin, Radiolysis of glutathione in oxygen-containing solutions of pH 7. Int. J. Radiat. Biol. 50, 195–202 (1977).CrossRefGoogle Scholar
  3. 3.
    M. Lai, 60Co-γ radiolysis of reduced glutathione in aerated solutions at pH values between 1–7.0. J. Chem. 1092–1097 (1976).Google Scholar
  4. 4.
    M. Tamba, M. Quintiliani, and G. Simone, Interactions of thiyl free radicals with oxygen: a pulse radiolysis study. Int. J. Radiat. Biol. 50, 595–600 (1986).CrossRefGoogle Scholar
  5. 5.
    J. Blok and W. S. D. Verhey, The attack of free radicals on biologically active DNA in irradiated aqueous solutions. Radiat. Res. 34, 689–703 (1968).PubMedCrossRefGoogle Scholar
  6. 6.
    J. De Jong, H. Loman and J. Blok, Inactivation of biologically active DNA by radiation-induced phenylalanine radicals. Int. J. Radiat. Biol. 22 11–21 (1972).CrossRefGoogle Scholar
  7. 7.
    R. L. Willson, Organic peroxy free radicals as ultimate agents in oxygen toxicity. In Oxidative Stress (H. Sies, Ed.) pp. 41–72, Academic Press, London, 1985.Google Scholar
  8. 8.
    G. Simone, J. C. M. Bremner, M. Tamba, B. R. Guerra, and M. Quintiliani, Thiol peroxyl radicals and radiation-induced enzyme inactivation. J. Radiat. Biol. 739 (1986) [Abstract].Google Scholar
  9. 9.
    P. Howards-Flanders, Effect of oxygen on the radiosensitivity of bacteriophage in the presence of sulphydryl compounds. Nature 186 485–487 (1960).CrossRefGoogle Scholar
  10. 10.
    K.D. Held, H. A. Harrop, and B. D. Michael, Effects of oxygen and sulphydryl-containing compounds on irradiated transforming DNA. Part II. Glutathione, cysteine and cysteamine. Int. J. Radiat. Biol. 40, 615–626 (1984).CrossRefGoogle Scholar
  11. 11.
    M. Tamba and M. Quintiliani, Kinetic studies of reactions involved in hydrogen transfer from glutathione to carbohydrate radicals. Radiat. Phys. Chem. 259–263 (1984).Google Scholar
  12. 12.
    R. J. Hodgkiss and R. W. Middleton, Enhancement of misonidazole radiosensitization by an inhibitor of glutathione biosynthesis. Int. J. Radiat. Biol. 179–183 (1983).Google Scholar
  13. 13.
    J. K. Dethmers and A. Meister, Glutathione export by human lymphoid cells: depletion of glutathione by inhibition of its synthesis decreases export and increases sensitivity to irradiation. Proc. Natl. Acad. Sci. 78, 7492–7496 (1981).PubMedCrossRefGoogle Scholar
  14. 14.
    J. E. Biaglow, M. E. Varnes, E. P. Clark and E. P. Epp, The role of thiols in cellular response to radiation and drugs. Radiat. Res. 437–455 (1983).Google Scholar
  15. 15.
    J. E. Biaglow, M. E. Varnes, E. P. Epp, E. P. Clark and M. Astor, Redox enzymes and thiol radicals. In Oxygen and Sulfur Radicals in Chemistry and Medicine (A. Breccia, M. A. J. Rodgers and G. Semerano, Eds.) pp. 89–102, Edizioni Scientifiche “Lo Scarabeo”, Bologna, Italy, 1986.Google Scholar
  16. 16.
    D. Ewing, T. M. Koval, and H. L. Walton, Radiation sensitization by oxygen of in vitro mammalian cells: is O\(\matrix{ {\overline \bullet } \cr 2 \cr } \) involved? Radiat. Res. 106, 356–365 (1986).PubMedCrossRefGoogle Scholar
  17. 17.
    H. Durchshlag and P. Zipper, Post-irradiation inactivation, protection, and repair of the sulfhydryl enzyme malate synthase. Effects of formate, superoxide dismutase, catalase and dithiothreitol. Radiat. Environ. Biophys. 24, 99–111 (1985).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • M. Tamba
    • 1
  • G. Simone
    • 1
  • M. Quintiliani
    • 1
    • 2
  1. 1.Istituto di Fotochimica e Radiazioni di Alta Energia (C.N.R.)BolognaItaly
  2. 2.Istituto T.B.M. (C.N.R.)RomaItaly

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