Advertisement

Differential Effects of 12-O-Tetradecanoylphorbol-13-Acetate (TPA) on Normal Initiated and Transformed Cells of a Single Mouse Keratinocyte Lineage

  • Molly Kulesz-Martin

Abstract

Previous studies of the differential response of normal, initiated and tumor cells to TPA have been performed on cell lines derived from normal epidermis, epidermis which had been treated in vivo with chemical carcinogen, or disaggregated tumor tissue. Thus, the cell lines were heterogeneous in cellular origin. In the present study, derivatives of a single cell lineage were examined for responsiveness to TPA at various stages of tumorigenic potential after chemical treatments in vitro. The advantage of this approach is that differences in target cell response can be related to defined stages of transformation, rather than individual variation among cells of different parental origins.

Keywords

Retinoic Acid Tumor Promoter Colony Number Tumorigenic Potential Tumor Cell Derivative 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    M. Kulesz-Martin, M.A. Yoshlda, L. Prestine, S.H. Yuspa and J.S. Bertram, Mouse cell clones for improved quantitation of carcinogen-induced altered differentiation. Carcinogenesis 6, 1245–1254 (1985).PubMedCrossRefGoogle Scholar
  2. 2.
    M. Kulesz-Martin, L. Blumenson and B. Lisafeld, Fetlnoic acid enhancement of an early step in the transformation of mouse epidermal cells in vitro. Carcinogenesis 7, 1425–1429 (1986).PubMedCrossRefGoogle Scholar
  3. 3.
    C.J. East and M. Kulesz-Martin, Retinole acid enhanced malignant potential of an initiated mouse keratinocyte cell line. Proc. Am. Assoc. Cancer Res. 28, 172 (1987).Google Scholar
  4. 4.
    S.H. Yuspa, T. Ben, H. Hennings and U. Lichti, Divergent responses in epidermal basal cells exposed to the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate. Cancer Res. 2344–2349 (1982).Google Scholar
  5. 5.
    S.H. Yuspa, D. Morgan, U. Lichti, E.F. Spangler, D. Michael, A. Kilkenny and H. Hennings, Cultivation and characterization of cells derived from mouse skin papillomas induced by an initiation-promotion protocol. Carcinogenesis 7, 949–958 (1986).PubMedCrossRefGoogle Scholar
  6. 6.
    S.H. Yuspa, H. Hennings, M. Kulesz-Martin and U. Lichti, The study of tumor promotion in a cell culture model for mouse skin-a tissue that exhibits multistage carcinogenesis in vivo. In: Carcinogenesis, Vol. 7 (E. Hecker, N.E. Fusenig, W. Kunz, F. Marks and H.W. Thielmann, Eds.), pp. 217–230. Raven Press, New York, 1982.Google Scholar
  7. 7.
    J.J. Reiners and T.J. Slaga, Effects of tumor promoters on the rate and commitment to terminal differentiation of subpopulations of murine keratinocytes. Cell 32, 247–255 (1983).PubMedCrossRefGoogle Scholar
  8. 8.
    F.K. Parkinson, P. Grabham and A. Emmerson, A subpopulation of cultured human keratinocytes which is resistant to the Induction of terminal differentiation-related changes by phorbol, 12-myristate, 13-acetate: evidence for an increase in the resistant population following transformation. Carcinogenesis 4, 857–861 (1983).PubMedCrossRefGoogle Scholar
  9. 9.
    P. Netteshelm, T. Gray and J.C. Barrett, The toxic response of preneoplastic rat tracheal epithelial cells to 12-O-tetradecanoyl-phorbol-13-acetate. Carcinogenesis 6, 1427–1434 (1985).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Molly Kulesz-Martin
    • 1
  1. 1.Grace Cancer Drug CenterRoswell Park Memorial InstituteBuffaloUSA

Personalised recommendations