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Mechanism of Action of Exonuclease III from Escherichia Coli

  • Yoke W. Kow
  • Hiroshi Ide
  • Susan S. Wallace

Abstract

Reactive oxygen species such as hydrogen peroxide, superoxide radical, and hydroxyl radical are formed during aerobic respiration (1). These reactive oxygen species can react with vital cell components such as the cell membrane, protein, as well as DNA, potentially leading to mutagenesis and cell death (1). In Escherichia coli. repair endonucleases. such as exonuclease III (2, 4) and endonuclease IV (3) appear to be involved in the repair of oxidative damages in DNA. Further, endonuclease III was shown to recognize oxidative base damages (4).

Keywords

Synthesize Heat Shock Protein Thymine Glycol Apurinic Site Glycosylic Linkage Oxidative Base Damage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    G. Cohen and R.A. Greenwald. Oxy Radicals and Their Scavenger Systems. Vol II. Elsevier Biomedical, New York. 1983.Google Scholar
  2. 2.
    Y.W. Kow and S.S. Wallace, Exonuclease III recognizes urea residues in oxidized DNA. Proc. Natl. Acad. Sci. (USA) 82:8354–8358 (1985).CrossRefGoogle Scholar
  3. 3.
    R.P. Cunningham, S.M. Saporito, S.C. Spitzer and B. Weiss, Endonuclease IV (nfo) mutant of Escherichia coli. J. Bacteriol. 168:1120–1127 (1986).PubMedGoogle Scholar
  4. 4.
    H.L. Katcher and S.S. Wallace. Characterization of the Escherichia coli X-ray endonuclease, endonuclease III. Biochemistry 22:4071–4080 (1983).PubMedCrossRefGoogle Scholar
  5. 5.
    K-H. Paek and G.C. Walker, Defect in expression of heat-shock proteins at high temperature in xthA mutants. J. Bacteriol. 164:1309–1316 (1986).Google Scholar
  6. 6.
    S.B. Farr, D.O. Natvig and R. Kagoma, Toxicity and mutagenesis of plumbagin and the induction of a possible new DNA repair pathway in Escherichia coli. J. Bacteriol. 165:763–770 (1985).Google Scholar
  7. 7.
    S.B. Farr, R. D’Ari and D. Touati. Oxygen-dependent mutagenesis in Escherichia coli lacking superoxide dismutase. Proc. Natl. Acad. Sci. (USA) 83:8268–8272 (1987).CrossRefGoogle Scholar
  8. 8.
    B. Demple, H.L. Halbrook and S. Linn, Escherichia coli xth mutants are hypersensitive to hydrogen peroxide. J. Bacteriol. 153:1079–1082 (1983).PubMedGoogle Scholar
  9. 9.
    L.J. Sammartano and R. W. Tuveson, Escherichia coli xthA mutants are sensitive to inactivation by broad spectrum near-UV (300-to 400-nm) radiation. J. Bacteriol. 156:904–906 (1983).PubMedGoogle Scholar
  10. 10.
    B. Weiss. In: The Enzymes. (P.D. Boyer Ed.) Vol. XIV pp. 203–231. Academic Press, New York, 1981.Google Scholar
  11. 11.
    R. Wu, G. Ruben, B. Siegel, E. Jay. P. Spielman and C.D. Tu, Synchronous digestion of SV40 DNA by exonuclease III. Biochemistry 15:734–739. (1976).PubMedCrossRefGoogle Scholar
  12. 12.
    S. Henikoff, Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359 (1986).CrossRefGoogle Scholar
  13. 13.
    B. Demple, A. Johnson and D. Fong, Exonuclease III and endonuclease IV remove 3’ blocks from DNA synthesis primers in HzOs-damaged Escherichia coli. Proc. Natl. Acad. Sci. (USA) 83:7731–7735 (1986).CrossRefGoogle Scholar
  14. 14.
    R. Roychoudbury and R. Wu, Novel properties of Escherichia coli exonuclease III. J. Biol. Chem. 252:4786–4789 (1977).Google Scholar
  15. 15.
    M. Liuzzi and M. Talpaert-Borle, A new approach to the study of the base-excision repair pathway using methoxyamine. J. Biol. Chem. 260:5252–5258 (1985).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Yoke W. Kow
    • 1
  • Hiroshi Ide
    • 1
  • Susan S. Wallace
    • 1
  1. 1.Department of Microbiology and ImmunologyNew York Medical CollegeValhallaUSA

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