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Intramuscular Injection of Plasmid DNA Expressing mRNA7 Coding the Nucleocapsid Protein of JHMV Partially Protected Mice Against Acute Infection with JHMV

  • M. Hayashi
  • K. Ishida
  • A. Maeda
  • Y. Kon
  • T. Mizutani
  • T. Watanabe
  • S. Arai
  • F. Okada
Chapter
  • 33 Downloads
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 440)

Abstract

We constructed a plasmid expressing mRNA7 coding the nucleocapsid (N) protein of JHM strain of mouse hepatitis virus (JHMV) under the control of Rous sarcoma virus LTR, referred to as pRSV-mRNA7. When C57BL/6 mice injected intramuscularly (i.m.) with control plasmid DNA which contained no viral sequence were infected with JHMV, necrotic figures of neural cells and diffuse immersion of lymphatic cells in the pedunculus cerebri were observed. In the hypothalamus, vascular cuffing consisting of lymphatic cells was observed. In contrast, no histological change was observed throughout these areas of the brains in the JHMV-infected mice after injection with pRSV-mRNA7. These results showed that the injection with plasmid DNA expressing mRNA7 of JHMV partially protected mice against acute infection with JHMV in the brain.

The plasmid DNA was i.m. injected into mice and the cytolytic activity of spleen cells from the mice was assessed by 51Cr-release assay. The spleen cells from the mice administrated with pRSV-mRNA7 showed a significant level of cytolytic activities against the transfected cells expressing the viral N protein even though the spleen cells were not cocultivated with stimulator cells. When the spleen cells from administrated mice with pRSV-mRNA7 were cocultivated with stimulator cells, higher cytolytic activity was observed against the transfected cells, compared with the activity without stimulation.

Keywords

Spleen Cell Intramuscular Injection Rabies Virus Cytolytic Activity Mouse Hepatitis Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • M. Hayashi
    • 1
  • K. Ishida
    • 2
  • A. Maeda
    • 2
  • Y. Kon
    • 2
  • T. Mizutani
    • 2
  • T. Watanabe
    • 2
  • S. Arai
    • 1
  • F. Okada
    • 3
  1. 1.Department of Veterinary RadiologyRakuno Gakuen UniversityEbetsuJapan
  2. 2.Laboratory of Experimental Animal Science, Graduate School of Veterinary MedicineHokkaido UniversitySapporoJapan
  3. 3.Laboratory of Pathology, Cancer Institute, School of MedicineHokkaido UniversitySapporoJapan

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