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Isolation of Hemagglutination-Defective Mutants for the Analysis of the Sialic Acid Binding Activity of Transmissible Gastroenteritis Virus

  • C. Krempl
  • M. L. Ballesteros
  • L. Enjuanes
  • G. Herrler
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 440)

Abstract

The surface protein S of transmissible gastroenteritis virus (TGEV) has a sialic acid binding activity that enables the virus to agglutinate erythrocytes. A protocol is described that has been successfully applied to the isolation of hemgglutination-defective mutants. The potential of these mutants for the characterization of the sialic acid-binding site and the function of the binding activity is discussed.

Keywords

Sialic Acid Infectious Bronchitis Virus Parental Virus Hemagglutinating Activity Acetyl Esterase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • C. Krempl
    • 1
  • M. L. Ballesteros
    • 1
  • L. Enjuanes
    • 2
  • G. Herrler
    • 1
  1. 1.Institut für VirologiePhilipps-Universität MarburgMarburgGermany
  2. 2.Centro Nacional de Biotecnologia, Department of Molecular and Cell Biology, CSICCampus Universidad Autonoma de MadridMadridSpain

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