Isolation of Hemagglutination-Defective Mutants for the Analysis of the Sialic Acid Binding Activity of Transmissible Gastroenteritis Virus

  • C. Krempl
  • M. L. Ballesteros
  • L. Enjuanes
  • G. Herrler
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 440)


The surface protein S of transmissible gastroenteritis virus (TGEV) has a sialic acid binding activity that enables the virus to agglutinate erythrocytes. A protocol is described that has been successfully applied to the isolation of hemgglutination-defective mutants. The potential of these mutants for the characterization of the sialic acid-binding site and the function of the binding activity is discussed.


Sialic Acid Infectious Bronchitis Virus Parental Virus Hemagglutinating Activity Acetyl Esterase 
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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • C. Krempl
    • 1
  • M. L. Ballesteros
    • 1
  • L. Enjuanes
    • 2
  • G. Herrler
    • 1
  1. 1.Institut für VirologiePhilipps-Universität MarburgMarburgGermany
  2. 2.Centro Nacional de Biotecnologia, Department of Molecular and Cell Biology, CSICCampus Universidad Autonoma de MadridMadridSpain

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