Isolation of Hemagglutination-Defective Mutants for the Analysis of the Sialic Acid Binding Activity of Transmissible Gastroenteritis Virus
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The surface protein S of transmissible gastroenteritis virus (TGEV) has a sialic acid binding activity that enables the virus to agglutinate erythrocytes. A protocol is described that has been successfully applied to the isolation of hemgglutination-defective mutants. The potential of these mutants for the characterization of the sialic acid-binding site and the function of the binding activity is discussed.
KeywordsSialic Acid Infectious Bronchitis Virus Parental Virus Hemagglutinating Activity Acetyl Esterase
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