Intrahepatic αβ-TcRintermediate LFA-1high T Cells are Stimulated During Mouse Hepatitis Viral Infection
- 30 Downloads
Mouse hepatitis virus type 3 (MHV3), a coronavirus, is an excellent animal model for the study of thymic and extrathymic T cell subpopulation disorders induced during the viral hepatitis. To understand local hepatic immune responses, the phenotypes of resident hepatic lymphocytes were determined and compared that of splenic and thymic T cell subpopulations during the acute viral hepatitis induced by MHV3 in susceptible C57BL/6 mice. Single positive (SP) CD4+ or CD8+ cells strongly increased in the liver. A specific cell population, the double positive (CD4+C8+) cells, normally present in liver and thymic cell preparations, decreased in C57BL/6 mice following the viral infection. αβ-TcRintermediateT cells shifted toward αβ-TcRhigh T cells in the liver and thymus of infected mice, but not in their spleen. The specific αβ-TcRint or high lymphocytes occurring in the liver of MHV3-infected mice expressed higher levels of leukocyte function antigen-1 (LFA-1) and Pgp-1 (CD44) activation markers, suggesting that they were either activated or antigen-experienced during the viral infection. No significant changes in T cell subpopulations were detected in the spleen. These observations suggest that MHV3 infection could induce an early in situ stimulation of resident hepatic T cells, despite a peripheral immunodeficiency in the thymus and spleen.
KeywordsInfected Mouse Cell Subpopulation Acute Viral Hepatitis Single Posi Murine Hepatitis Virus
- Pereira, C.A., Steffan, A.M., and Kirn, A., 1984, Interaction between mouse hepatitis virus and primary cultures of Kupffer and endothelial liver cells from resistant and susceptible inbred mouse strains, J. Gen. Virol. 65:35–41.Google Scholar
- Pham, B.N., Martinot-Peignoux, M., Mosnier, J.F., Njapoum, C, Marceliin, P., Bougy, F., Degott, C, Erlinger, S., Cohen, J.H., and Degos, F., 1995, CD4+/CD8+ ratio of liver-derived lymphocytes is related to viraemia and not to hepatitis C virus genotypes in chronic hepatitis C, Clin. Exp. Immunol. 102:320–356.PubMedCrossRefGoogle Scholar