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Intrahepatic αβ-TcRintermediate LFA-1high T Cells are Stimulated During Mouse Hepatitis Viral Infection

  • L. Lamontagne
  • E. Massicotte
  • C. Page
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 440)

Abstract

Mouse hepatitis virus type 3 (MHV3), a coronavirus, is an excellent animal model for the study of thymic and extrathymic T cell subpopulation disorders induced during the viral hepatitis. To understand local hepatic immune responses, the phenotypes of resident hepatic lymphocytes were determined and compared that of splenic and thymic T cell subpopulations during the acute viral hepatitis induced by MHV3 in susceptible C57BL/6 mice. Single positive (SP) CD4+ or CD8+ cells strongly increased in the liver. A specific cell population, the double positive (CD4+C8+) cells, normally present in liver and thymic cell preparations, decreased in C57BL/6 mice following the viral infection. αβ-TcRintermediateT cells shifted toward αβ-TcRhigh T cells in the liver and thymus of infected mice, but not in their spleen. The specific αβ-TcRint or high lymphocytes occurring in the liver of MHV3-infected mice expressed higher levels of leukocyte function antigen-1 (LFA-1) and Pgp-1 (CD44) activation markers, suggesting that they were either activated or antigen-experienced during the viral infection. No significant changes in T cell subpopulations were detected in the spleen. These observations suggest that MHV3 infection could induce an early in situ stimulation of resident hepatic T cells, despite a peripheral immunodeficiency in the thymus and spleen.

Keywords

Infected Mouse Cell Subpopulation Acute Viral Hepatitis Single Posi Murine Hepatitis Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • L. Lamontagne
    • 1
  • E. Massicotte
    • 1
  • C. Page
    • 1
  1. 1.Département des Sciences BiologiquesUniversité du Québec à MontréalMontréalCanada

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