Coronavirus MHV-A59 Causes Upregulation of Interferon-ß RNA in Primary Glial Cell Cultures
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Infection of mice with Coronavirus mouse hepatitis virus strain MHV-A59 causes focal acute encephalitis, hepatitis and chronic demyelinating disease. To investigate host in-terferon (IFN) response to viral infection within the brain, RNA was extracted from A59-or MHV-2- infected and mock-infected primary astrocyte cultures from newborn mice, RT-PCR amplified RNA with primers specific for the various IFNs, transferred to nylon membranes and hybridized with IFN specific digoxigenin-labeled probes. Infection of primary astrocyte cultures from newborn mice with either A59 or MHV-2 caused upregulation of IFN-ß RNA, but not IFN-γ or IFN-α. Thus, brain astrocytes are capable of producing a local IFN-ß response upon infection with MHV. The response of the other IFNs following MHV infection may be derived from inflammatory cells.
KeywordsNewcastle Disease Virus Newborn Mouse Mouse Hepatitis Virus Primary Astrocyte Culture Brain Astrocyte
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