Coronavirus MHV-A59 Causes Upregulation of Interferon-ß RNA in Primary Glial Cell Cultures

  • Q. Wang
  • J. A. Haluskey
  • E. Lavi
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 440)


Infection of mice with Coronavirus mouse hepatitis virus strain MHV-A59 causes focal acute encephalitis, hepatitis and chronic demyelinating disease. To investigate host in-terferon (IFN) response to viral infection within the brain, RNA was extracted from A59-or MHV-2- infected and mock-infected primary astrocyte cultures from newborn mice, RT-PCR amplified RNA with primers specific for the various IFNs, transferred to nylon membranes and hybridized with IFN specific digoxigenin-labeled probes. Infection of primary astrocyte cultures from newborn mice with either A59 or MHV-2 caused upregulation of IFN-ß RNA, but not IFN-γ or IFN-α. Thus, brain astrocytes are capable of producing a local IFN-ß response upon infection with MHV. The response of the other IFNs following MHV infection may be derived from inflammatory cells.


Newcastle Disease Virus Newborn Mouse Mouse Hepatitis Virus Primary Astrocyte Culture Brain Astrocyte 
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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Q. Wang
    • 1
  • J. A. Haluskey
    • 1
  • E. Lavi
    • 1
  1. 1.Division of Neuropathology, Department of Pathology and Laboratory Medicine, School of MedicineUniversity of PennsylvaniaPhiladelphiamUSA

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