Interactions of Enterotropic Mouse Hepatitis Viruses with Bgp2 Receptor Proteins

  • Susan R. Compton
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 440)


Enterotropic mouse hepatitis virus (MHV) infections are limited to the intestinal mucosa, rarely disseminate to other tissues and cause disease only in neonatal mice.The role of virus-host cell receptor interactions in the limited tissue tropism of enterotropic MHV infections is unclear. Previous studies have shown that enterotropic MHV-Y can infect BHK cells stably transfected with either the MHVR or the mmCGM2 receptor gene. In contrast, enterotropic MHV-RI infects BHK cells stably transfected with the MHVR but not the mmCGM2 receptor gene. Studies to determine whether MHV-Y and -RI can utilize the Bgp2 receptor isoform were performed. Both MHV-Y and -RI infected Vero cells transiently transfected with the Bgp2 receptor gene, though only MHV-Y infected CHO cells stably transfected with the Bgp2 receptor gene. Additionally, pretreatment with anti-MHVR monoclonal antibody (CC1) did not prevent MHV-Y and -RI infection of CMT93 cells. In contrast, pretreatment with CC1 prevented MHV-A59 infection of CMT93 cells. It is likely that MHV-Y and -RI use the Bgp2 receptor to infect CC1 pretreated CMT93 cells, since CMT93 cells are known to possess high levels of the Bgp2 receptor mRNAs, but it is also possible that they use an unidentified receptor.


Functional Receptor Receptor Binding Domain Mouse Hepatitis Virus Murine Coronavirus CMT93 Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Barthold, S.W., 1987, Host age and genotypic effects on enterotropic mouse hepatitis virus infection, Lab. Anim. Sci. 37:36–40.PubMedGoogle Scholar
  2. Barthold, S.W. and A.L. Smith., 1987, Response of genetically susceptible and resistant mice to intranasal inoculation with mouse hepatitis virus JHM, Virus Re. 7:225–239.PubMedCrossRefGoogle Scholar
  3. Barthold, S.W., A.L. Smith, P.F.S. Lord, P.N. Bhatt, R.O. Jacoby, and A.J. Main., 1982, Epizootic coronaviral typhlocolitis in suckling mice, Lab. Anim. Sci. 32:376-383.PubMedGoogle Scholar
  4. Barthold, S.W., A.L. Smith and M.L. Povar., 1985, Enterotropic mouse hepatitis virus infection in nude mice, Lab. Anim. Sci. 35:613–618.PubMedGoogle Scholar
  5. Barthold, S.W., D.S. Beck and A.L. Smith., 1993, Enterotropic Coronavirus (mouse hepatitis virus) in mice: influence of host age and strain on infection and disease, Lab. Anim. Sci. 43:276–284.PubMedGoogle Scholar
  6. Beauchemin, N., C. Turbide, D. Afar, J. Bell, M. Raymond, CR Stanners and A. Fuks., 1989, A mouse analogue of the human carcinoembryonic antigen, Cancer Res. 49:2017–2021.PubMedGoogle Scholar
  7. Boyle, J.F., Weismiller, D.G. and Holmes, K.V., 1987, Genetic resistance to mouse hepatitis virus correlates with absence of virus-binding activity on target tissues, J. Virol. 61:185–189.PubMedGoogle Scholar
  8. Chen, D.S., Asanaka, M., Yokomori, K., Wang, F.-I., Hwang, S.B., Li, H.-P. and Lai, M.M.C., 1995, A pregnancy-specific glycoprotein is expressed in the brain and serves as a receptor for mouse hepatitis virus, Proc. Natl. Acad. Sci. USA 92:12095–12099.PubMedCrossRefGoogle Scholar
  9. Chen, D.S., Asanaka, M., Chen, F.S., Shively, J.E. and Lai, M.M.C., 1997, Human carcinoembryonic antigen and biliary glycoprotein can serve as mouse hepatitis virus receptors, J. Virol. 71:1688–1691.PubMedGoogle Scholar
  10. Compton, S.R., S.W. Barthold, and AL. Smith., 1993, The cellular and molecular pathogenesis of coronaviruses, Lab. Anim. Sci. 43:1–14.Google Scholar
  11. Compton, S.R., 1994, Enterotropic strains of mouse Coronavirus differ in their use of murine carcinoembryonic antigen-related glycoprotein receptors, Virology 203:197–201.PubMedCrossRefGoogle Scholar
  12. Dveksler, G.S., M.N. Pensiero, C.B. Cardellichio, R.K. Williams, G-S. Jiang, K.V. Holmes, and C.W Dieffenbach., 1991, Cloning of the mouse hepatitis virus (MHV) receptor: expression in human and hamster cell lines confers susceptibility to MHV, J. Virol. 65:6881–6891.PubMedGoogle Scholar
  13. Dveksler, G.S., C.W. Dieffenbach, C.B. Cardellichio, K. McCuaig, M.N. Pensiero, G-S. Jiang, N. Beauchemin, and K.V. Holmes., 1993a, Several members of the mouse carcinoembryonic antigen-related glycoprotein family are functional receptors for mouse hepatitis virus -A59, J. Virol. 67:1–8.PubMedGoogle Scholar
  14. Dveksler, G.S., M.N. Pensiero, C.W. Dieffenbach, C.B. Cardellichio, A.A. Basile, P.E. Elia and K.V. Holmes., 1993b, Mouse hepatitis virus strain A59 and blocking antireceptor monoclonal antibody bind to the N-terminal domain of cellular receptor, Proc. Natl. Acad. Sci. USA. 90:1716–1720.PubMedCrossRefGoogle Scholar
  15. Dveksler, G.S., A.A. Basile, C.B. Cardellichio and K.V. Holmes., 1995, Mouse hepatitis virus receptor activities of an MHVR/mph chimera and MHVR mutants lacking N-linked glycosylation of the N-terminal domain. J. Virol. 69:543–546.PubMedGoogle Scholar
  16. Dveksler, G.S., S.E. Gagneten, CA. Scanga, C.B. Cardellichio and K.V. Holmes., 1996, Expression of the recombinant anchorless N-terminal domain of mouse hepatitis virus (MHV) receptor makes hamster or human cells susceptible to MHV infection, J. Virol. 70:4142–4145.PubMedGoogle Scholar
  17. Gallagher, T.M., Buchmeier, M.J. and Perimän, S., 1992, Cell receptor-independent infection by a neurotropic murine Coronavirus, Virology 191:517–522.PubMedCrossRefGoogle Scholar
  18. Godfraind, C, Langreth, S.G., Cardellichio, C.B., Knobler, R.L., Coutelier, J.P., Dubois-Dalcq, M. and Holmes, K.V, 1995, Tissue and cellular distribution of an adhesion molecule in the carcinoembryonic antigen family that serves as a receptor for mouse hepatitis virus, Lab. Invest. 73:615–627.PubMedGoogle Scholar
  19. Kubo, H., Yamada, Y.K. and Taguchi, F., 1994, Localization of neutralizing epitopes and receptor-binding site within the amino-terminal 330 amino acids of the murine Coronavirus spike protein, J. Virol. 68:5403–5410.PubMedGoogle Scholar
  20. Kunita, S., Zhang, L., Hornberger, F.R. and Compton, S.R., 1995, Molecular characterization of the S proteins of two enterotropic murine Coronavirus strains, Virus Res. 35:277–289.PubMedCrossRefGoogle Scholar
  21. McCuaig, K., C. Turbide and N. Beauchemin., 1992, mmCGMla: a mouse carcinoembryonic antigen gene family member, generated by alternative splicing, functions as an adhesion molecule, Cell Growth Differ. 3:165–174.PubMedGoogle Scholar
  22. McCuaig, K., M. Rosenberg, P. Nedellec, C Turbide and N. Beauchemin., 1993, Expression of the Bgp gene and characterization of mouse colon biliary glycoprotein isoforms, Gene 127:173–183.PubMedCrossRefGoogle Scholar
  23. Nash, T.C and Buchmeier, M.J., 1996, Spike glycoprotein-mediated fusion in biliary glycoprotein-independent cell-associated spread of mouse hepatitis virus infection, Virology 223:68–78.PubMedCrossRefGoogle Scholar
  24. Nedellec, P., G.S. Dveksler, E. Daniels, C Turbide, B. Chow, A.A. Basile, K.V. Holmes and N. Beauchemin., 1994, Bgp2, a new member of the carcinoembryonic antigen-related gene family, encodes an alternative receptor for mouse hepatitis viruses,.J. Virol. 68: 4525–4537.PubMedGoogle Scholar
  25. Ohtsuka, N., Yamada, Y.K. and Taguchi, F., 1996, Difference in virus-binding activity to two distinct receptor proteins for mouse hepatitis virus, J. Gen. Virol. 77:1683–1692.PubMedCrossRefGoogle Scholar
  26. Suzuki, H. and Taguchi, F., 1996, Analysis of the receptor-binding site of murine Coronavirus spike protein, J. Virol. 70:2632–2636.PubMedGoogle Scholar
  27. Turbide, C, M. Rojas, CP. Stanners and N. Beauchemin., 1991, A mouse carcinoembryonic antigen gene family member is a calcium-dependent cell adhesion molecule, J. Biol. Chem. 266:309–315.PubMedGoogle Scholar
  28. Williams R.K., G-S. Jiang, S.W. Snyder, M.F. Frana, and K.V. Holmes., 1990, Purification of the 110-kilodalton glycoprotein receptor for mouse hepatitis virus (MHV)-A59 from mouse liver and identification of a nonfunctional homologous protein in MHV-resistant SJL/J mice, J. Virol. 64:3817–3823.PubMedGoogle Scholar
  29. Williams R.K., G-S. Jiang and K.V. Holmes., 1991, Receptor for mouse hepatitis virus is a member of the carci-noembryonic antigen family of glycoproteins, Proc. Natl. Acad. Sci. USA 88:5533–5536.PubMedCrossRefGoogle Scholar
  30. Yokomori, K. and M.M.C. Lai., 1992a, Mouse hepatitis virus utilizes two carcinoembryonic antigens as alternative receptors, J. Virol. 66:6194–6199.PubMedGoogle Scholar
  31. Yokomori, K. and M.M.C. Lai., 1992b, The receptor for mouse hepatitis virus in the resistant mouse strain SJL is functional: implications for the requirement of a second factor for viral infection, J. Virol. 66:6931–6938.PubMedGoogle Scholar
  32. Yokomori, K., Asanaka, M., Stohlman, S.A. and Lai, M.M.C, 1993, A spike protein-dependent cellular factor other than the viral receptor is required for mouse hepatitis virus entry, Virology 196:45–56.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Susan R. Compton
    • 1
  1. 1.Section of Comparative MedicineYale University School of MedicineNew HavenUSA

Personalised recommendations